Over-expression of a chimeric gene of the transcriptional co-activator MBF1 fused to the EAR repressor motif causes developmental alteration in Arabidopsis and tomato

Hommel, Marie; Khalil-Ahmad, Qaiser; Jaimes-Miranda, Fabiola; Mila, Isabelle; Pouzet, Cécile; Latché, Alain; Pech, Jean‐Claude; Bouzayen, Mondher; Regad, Farid

doi:10.1016/j.plantsci.2008.01.019

Cited by 15 publications

(14 citation statements)

References 38 publications

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“…Fusion of tomato SlER24 to EAR (Amphiphilic Repression) in the MicroTom cultivar induced a delay of seed germination, but had no obvious effect on plant growth [6]. Moreover, it was reported that the StMBF1 gene in potato was induced by pathogen attack, oxidative stress, wounding and in response to salicylic acid (SA) treatment [7,8].…”

Section: Introductionmentioning

confidence: 99%

Reduced tolerance to abiotic stress in transgenic Arabidopsis overexpressing a Capsicum annuummultiprotein bridging factor 1

Guo

Chen

et al. 2014

BMC Plant Biol

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BackgroundThe pepper fruit is the second most consumed vegetable worldwide. However, low temperature affects the vegetative development and reproduction of the pepper, resulting in economic losses. To identify cold-related genes regulated by abscisic acid (ABA) in pepper seedlings, cDNA representational difference analysis was previously performed using a suppression subtractive hybridization method. One of the genes cloned from the subtraction was homologous to Solanum tuberosum MBF1 (StMBF1) encoding the coactivator multiprotein bridging factor 1. Here, we have characterized this StMBF1 homolog (named CaMBF1) from Capsicum annuum and investigated its role in abiotic stress tolerance.ResultsTissue expression profile analysis using quantitative RT-PCR showed that CaMBF1 was expressed in all tested tissues, and high-level expression was detected in the flowers and seeds. The expression of CaMBF1 in pepper seedlings was dramatically suppressed by exogenously supplied salicylic acid, high salt, osmotic and heavy metal stresses. Constitutive overexpression of CaMBF1 in Arabidopsis aggravated the visible symptoms of leaf damage and the electrolyte leakage of cell damage caused by cold stress in seedlings. Furthermore, the expression of RD29A, ERD15, KIN1, and RD22 in the transgenic plants was lower than that in the wild-type plants. On the other hand, seed germination, cotyledon greening and lateral root formation were more severely influenced by salt stress in transgenic lines compared with wild-type plants, indicating that CaMBF1-overexpressing Arabidopsis plants were hypersensitive to salt stress.ConclusionsOverexpression of CaMBF1 in Arabidopsis displayed reduced tolerance to cold and high salt stress during seed germination and post-germination stages. CaMBF1 transgenic Arabidopsis may reduce stress tolerance by downregulating stress-responsive genes to aggravate the leaf damage caused by cold stress. CaMBF1 may be useful for genetic engineering of novel pepper cultivars in the future.

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Section: Introductionmentioning

confidence: 99%

Reduced tolerance to abiotic stress in transgenic Arabidopsis overexpressing a Capsicum annuummultiprotein bridging factor 1

Guo

Chen

et al. 2014

BMC Plant Biol

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“…Thin transverse 5th internodal sections of B. balcooa from representative accessions of 12 locations were incubated in a 0.2% aqueous solution of Congo red (CR) (Merck, Darmstadt, Germany) overnight in the dark (Hommel et al, 2008). The samples were then washed with deionized water, and images were acquired using CLSM with a confocal pinhole set at 'Airy' 1.0.…”

Section: Congo Red Staining Of Internodal Sectionsmentioning

confidence: 99%

Screening of superior fiber-quality-traits among wild accessions of Bambusa balcooa: efficient and non-invasive evaluation of fiber developmental stages

et al. 2010

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Abstract• The search for alternative fiber resources is receiving worldwide attention for the potential role of these fibers in climate and forest restoration. Among bamboos, Bambusa balcooa is generally preferred for industrial use due to its rapid growth, excellent flexibility and tensile strength. The aim of this study is to establish a non-invasive and efficient method of screening superior fiber quality from the wild gene pool of B. balcooa.• Anatomical features of B. balcooa internodes were revealed by light microscopy. Confocal laser scanning microscopy (CLSM) was used to study fiber developmental stages in emerging branches using the intrinsic fluorescence properties of lignin and chlorophyll. Physical properties of B. balcooa fibers from different gene pools were studied, and biochemical estimations of lignin and cellulose contents were performed. CLSM-based semi-quantitative in situ estimations of cellulose and lignin contents in fiber bundles were performed using orthochromatic Congo red (CR) and acridine orange (AO) labeling, respectively.• Fibers isolated from representative accessions of locations 3 and 4 showed the highest cellulose contents and superior physical properties. The results obtained from biochemical analyses of cellulose and lignin contents of fiber bundles among the accessions correlate significantly with CR and AO fluorescence values using non-invasive methods. A CLSM-based study demonstrated the possibility of differentiating stages of fiber development with autofluorescent markers of lignin and lignin precursors that would help to define stages of fiber development and maturation in bamboo effectively, which is a prerequisite for understanding the mechanism of fiber development at the molecular level in bamboo.• A CLSM-based cellulose and lignin assay is a non-invasive method, exploited for the first time for genetic diversity screening in a wild gene pool. This protocol would also be useful for rapid resource screening for various commercial purposes.

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“…A modified EAR motif, called SRDX (modified SUPRD with the sequence LDLDLELRLGFA; SUPRD is a 30 residue C-terminal region of SUPERMAN (SUP) from Arabidopsis, which acts as a repression domain (RD)) and a 32 residue LxLxL-type EAR motif (LDLNLELRISPP) have been successfully used as a dominant repressor in fusion with TFs, to identify the functionality of redundant TFs [24,25]. This technique popularly referred to as chimeric repressor silencing technology (CRES-T) can also be exploited for co-activators, which do not have DNA binding capacity [26]. The LxLxL motif in Aux/IAA (Auxin/Indole-3-acetic acid) proteins is transferable and dominant over activation domains.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Rice Proteins with DLN Repressor Motif/S

Singh

Mathew

Verma

et al. 2019

IJMS

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Transcriptional regulation includes both activation and repression of downstream genes. In plants, a well-established class of repressors are proteins with an ERF-associated amphiphilic repression/EAR domain. They contain either DLNxxP or LxLxL as the identifying hexapeptide motif. In rice (Oryza sativa), we have identified a total of 266 DLN repressor proteins, with the former motif and its modifications thereof comprising 227 transcription factors and 39 transcriptional regulators. Apart from DLNxxP motif conservation, DLNxP and DLNxxxP motifs with variable numbers/positions of proline and those without any proline conservation have been identified. Most of the DLN repressome proteins have a single DLN motif, with higher relative percentage in the C-terminal region. We have designed a simple yeast-based experiment wherein a DLN motif can successfully cause strong repression of downstream reporter genes, when fused to a transcriptional activator of rice or yeast. The DLN hexapeptide motif is essential for repression, and at least two “DLN” residues cause maximal repression. Comparatively, rice has more DLN repressor encoding genes than Arabidopsis, and DLNSPP motif from rice is 40% stronger than the known Arabidopsis SRDX motif. The study reports a straightforward assay to analyze repressor activity, along with the identification of a strong DLN repressor from rice.

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Over-expression of a chimeric gene of the transcriptional co-activator MBF1 fused to the EAR repressor motif causes developmental alteration in Arabidopsis and tomato

Cited by 15 publications

References 38 publications

Reduced tolerance to abiotic stress in transgenic Arabidopsis overexpressing a Capsicum annuummultiprotein bridging factor 1

Reduced tolerance to abiotic stress in transgenic Arabidopsis overexpressing a Capsicum annuummultiprotein bridging factor 1

Screening of superior fiber-quality-traits among wild accessions of Bambusa balcooa: efficient and non-invasive evaluation of fiber developmental stages

Analysis of Rice Proteins with DLN Repressor Motif/S

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