Ovarian maturation stages of the mud crab Scylla serrata

Abstract

Ovarian maturation in adult wild‐sourced and pond‐grown Scylla serrata (Forsskål) was determined based on gross morphology and histological appearance. There were no significant differences noted in the histological features of both wild and pond‐reared S. serrata females. Ovarian maturation was classified into five stages: immature, early maturing, late maturing, fully mature and spent. The immature ovaries are thin and translucent to off white and contain oogonia, primary oocytes with large nuclei. The follicle cells were found around the periphery of the lobes and an area among groups of oogonia and oocytes. The follicle cells gradually enclosed the oocytes. The early‐maturing ovaries were yellow and small yolk globules started to appear in larger oocytes. In late‐maturing ovaries, the colour became light orange and lobules were apparent. Yolk globules occurred in the cytoplasm with larger globular inclusions towards the periphery, while follicle cells were hardly recognizable. Fully mature ovaries were orange to deep orange and had swollen lobules. Large yolk globules were apparent in the entire cytoplasm. Follicle cells were hardly seen. Spent ovaries were similar to the early‐maturing and late‐maturing stage in partially spawned females. The ovarian development was correlated closely to the gonadosomatic index, oocyte diameter, and ovarian histology. The classification of ovarian maturation provides baseline information for further studies on reproductive biology. Likewise, the information provides a guide for broodstock management in the hatchery.

Total Bacterial Load and Enumeration of Pathogenic Bacteria vibrio spp., Aeromonas spp. and Pseudomonas spp. in the Production Line of Mud Crab (Scylla olivacea) Hatchery in Bangladesh

“…The TVC, TAC and TPC were found almost similar to the findings of Interminense et al (2014). Overall, in the present study, the load of pathogenic Vibrio, Aeromonas, and Pseudomonas identified in the different components of the hatchery was similar to the findings of many authors (Nakamura et al, 1995;Roza and Hatai, 1999;Lavilla-pitogo and de la Pena, 2004;Quinitio and Parado-Estepa, 2008;Dan and Hamasaki, 2015) who conducted such experiments in the production line, particularly with S. serrata and S. paramamosain.…”

Total Bacterial Load and Enumeration of Pathogenic Bacteria vibrio spp., Aeromonas spp. and Pseudomonas spp. in the Production Line of Mud Crab (Scylla olivacea) Hatchery in Bangladesh

“…The TVC, TAC and TPC were found almost similar to the findings of Interminense et al (2014). Overall, in the present study, the load of pathogenic Vibrio, Aeromonas, and Pseudomonas identified in the different components of the hatchery was similar to the findings of many authors (Nakamura et al, 1995;Roza and Hatai, 1999;Lavilla-pitogo and de la Pena, 2004;Quinitio and Parado-Estepa, 2008;Dan and Hamasaki, 2015) who conducted such experiments in the production line, particularly with S. serrata and S. paramamosain.…”

Nutrient Composition of Ovary, Hepatopancreas and Muscle Tissues in Relation to Ovarian Development Stage of Female Swimming Crab, Portunus trituberculatus

“…GSI, HSI, and Ovary Maturation P. trituberculatus ovarian maturation was classified into five distinct stages based on external morphology and ovary color. Similar classification systems have been reported for other species of female crabs such as Scylla serrata [33], S. paramamosain [34], and S. olivacea [35]. The GSI, which serves as an indicator of gonadal maturation in P. trituberculatus [36], showed a clear increase across the different ovary stages observed in the present study.…”

Ovarian Maturation Stages of Wild and Captive Mud Crab, Scylla olivacea Fed with Two Diets

“…The oocyte size of S. olivacea also significantly increased as the maturation of ovary progressed. These results are similar to those observed in gonad of mud crab, S. serrata (Forskål 1775) (Quinitio et al 2007), mud crab, S. olivacea (Islam et al 2010a) and mud crab, S. paramamosain (Estampador 1949) (Islam et al 2010b). The present of many follicle cells and large nucleus of the oocytes in the immature and early maturing of S. olivacea indicate that the early stages of ovarian maturation are involved the yolk synthesis has been reported in other crustacean such S. olivacea (Islam et al 2010a), S. paramamosain (Islam et al 2010b) and mantis shrimp, Oratosquillao ratoria (De Haan, 1844) (Kodama et al 2004).…”

Total Bacterial Load and Enumeration of Pathogenic Bacteria vibrio spp., Aeromonas spp. and Pseudomonas spp. in the Production Line of Mud Crab (Scylla olivacea) Hatchery in Bangladesh

“…The TVC, TAC and TPC were found almost similar to the findings of Interminense et al (2014). Overall, in the present study, the load of pathogenic Vibrio, Aeromonas, and Pseudomonas identified in the different components of the hatchery was similar to the findings of many authors (Nakamura et al, 1995;Roza and Hatai, 1999;Lavilla-pitogo and de la Pena, 2004;Quinitio and Parado-Estepa, 2008;Dan and Hamasaki, 2015) who conducted such experiments in the production line, particularly with S. serrata and S. paramamosain.…”

Nutrient Composition of Ovary, Hepatopancreas and Muscle Tissues in Relation to Ovarian Development Stage of Female Swimming Crab, Portunus trituberculatus

“…GSI, HSI, and Ovary Maturation P. trituberculatus ovarian maturation was classified into five distinct stages based on external morphology and ovary color. Similar classification systems have been reported for other species of female crabs such as Scylla serrata [33], S. paramamosain [34], and S. olivacea [35]. The GSI, which serves as an indicator of gonadal maturation in P. trituberculatus [36], showed a clear increase across the different ovary stages observed in the present study.…”

Ovarian Maturation Stages of Wild and Captive Mud Crab, Scylla olivacea Fed with Two Diets

“…The oocyte size of S. olivacea also significantly increased as the maturation of ovary progressed. These results are similar to those observed in gonad of mud crab, S. serrata (Forskål 1775) (Quinitio et al 2007), mud crab, S. olivacea (Islam et al 2010a) and mud crab, S. paramamosain (Estampador 1949) (Islam et al 2010b). The present of many follicle cells and large nucleus of the oocytes in the immature and early maturing of S. olivacea indicate that the early stages of ovarian maturation are involved the yolk synthesis has been reported in other crustacean such S. olivacea (Islam et al 2010a), S. paramamosain (Islam et al 2010b) and mantis shrimp, Oratosquillao ratoria (De Haan, 1844) (Kodama et al 2004).…”

Total Bacterial Load and Enumeration of Pathogenic Bacteria vibrio spp., Aeromonas spp. and Pseudomonas spp. in the Production Line of Mud Crab (Scylla olivacea) Hatchery in Bangladesh

“…The TVC, TAC and TPC were found almost similar to the findings of Interminense et al (2014). Overall, in the present study, the load of pathogenic Vibrio, Aeromonas, and Pseudomonas identified in the different components of the hatchery was similar to the findings of many authors (Nakamura et al, 1995;Roza and Hatai, 1999;Lavilla-pitogo and de la Pena, 2004;Quinitio and Parado-Estepa, 2008;Dan and Hamasaki, 2015) who conducted such experiments in the production line, particularly with S. serrata and S. paramamosain.…”

Nutrient Composition of Ovary, Hepatopancreas and Muscle Tissues in Relation to Ovarian Development Stage of Female Swimming Crab, Portunus trituberculatus

“…GSI, HSI, and Ovary Maturation P. trituberculatus ovarian maturation was classified into five distinct stages based on external morphology and ovary color. Similar classification systems have been reported for other species of female crabs such as Scylla serrata [33], S. paramamosain [34], and S. olivacea [35]. The GSI, which serves as an indicator of gonadal maturation in P. trituberculatus [36], showed a clear increase across the different ovary stages observed in the present study.…”

Ovarian Maturation Stages of Wild and Captive Mud Crab, Scylla olivacea Fed with Two Diets

“…The oocyte size of S. olivacea also significantly increased as the maturation of ovary progressed. These results are similar to those observed in gonad of mud crab, S. serrata (Forskål 1775) (Quinitio et al 2007), mud crab, S. olivacea (Islam et al 2010a) and mud crab, S. paramamosain (Estampador 1949) (Islam et al 2010b). The present of many follicle cells and large nucleus of the oocytes in the immature and early maturing of S. olivacea indicate that the early stages of ovarian maturation are involved the yolk synthesis has been reported in other crustacean such S. olivacea (Islam et al 2010a), S. paramamosain (Islam et al 2010b) and mantis shrimp, Oratosquillao ratoria (De Haan, 1844) (Kodama et al 2004).…”