Outcomes and prognostic factors in gallbladder cancer: a single-centre experience

Cziupka, Katharina; Partecke, Lars Ivo; Mirow, Lutz; Heidecke, Claus–Dieter; Emde, C.; Hoffmann, Wolfgang; Siewert, Ulrike; Berg, Neeltje van den; Bernstorff, Wolfram von; Stier, A.

doi:10.1007/s00423-012-0950-8

Cited by 28 publications

(26 citation statements)

References 21 publications

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“…The mean survival for this malignancy ranges from 5.2 to 24.4 mo, according to different reports. 9,[11][12][13] Most of these patients die from tumor metastasis and recurrence. Therefore, an understanding of the molecular mechanisms of GBC metastasis and recurrence is indispensable for the development of effective adjuvant therapy.…”

Section: Introductionmentioning

confidence: 99%

MALAT1 promotes the proliferation and metastasis of gallbladder cancer cells by activating the ERK/MAPK pathway

Wang

et al. 2014

Cancer Biology & Therapy

210

181

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Section: Introductionmentioning

confidence: 99%

MALAT1 promotes the proliferation and metastasis of gallbladder cancer cells by activating the ERK/MAPK pathway

Wang

et al. 2014

Cancer Biology & Therapy

210

181

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“…The poor prognosis of gallbladder carcinoma is not only because of its low radical resection rate but also its lack of sensitivity to chemotherapy and radiotherapy [11][12][13][14][15][16]. Therefore, novel therapeutic strategies are needed to improve patient outcomes [26].…”

Section: Discussionmentioning

confidence: 99%

“…Curative resection remains the only cure for this highly lethal malignancy. As a result, gallbladder carcinoma is associated with a very poor prognosis, and the overall survival rate for this malignancy ranges from 5.2 to 24.4 months [11][12][13]. Therefore, only a minority of patients are candidates for curative resection.…”

Section: Introductionmentioning

confidence: 99%

Effects of oxymatrine on the apoptosis and proliferation of gallbladder cancer cells

Yang²,

Gu³

et al. 2014

Anti-Cancer Drugs

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Gallbladder carcinoma is the most common malignancy of the biliary tract and is associated with a very poor outcome. The aim of the present study was to investigate the effects of oxymatrine (OM) on gallbladder cancer cells and the possible mechanism of its effects. The effects of OM on the proliferation of gallbladder cancer cells (GBC-SD and SGC-996) were investigated using cell counting kit-8 and colony formation assays. Annexin V/propidium iodide double staining was performed to investigate whether OM could induce apoptosis in gallbladder cancer cells. The mitochondrial membrane potential (ΔΨm) and expression of apoptosis-associated proteins were evaluated to identify a mechanism for the effects of OM. In addition, the RNA expression of relevant genes was measured by qRT-PCR using the SYBR Green method. Finally, a subcutaneous implantation model was used to verify the effects of OM on tumor growth in vivo. We found that OM inhibited the proliferation of gallbladder cancer cells. In addition, Annexin V/propidium iodide double staining showed that OM induced apoptosis after 48 h and the ΔΨm decreased in a dose-dependent manner after OM treatment. Moreover, the activation of caspase-3 and Bax and downregulation of Bcl-2 and nuclear factor κB were observed in OM-treated cells. Finally, OM potently inhibited in-vivo tumor growth following subcutaneous inoculation of SGC-996 cells in nude mice. In conclusion, OM treatment reduced proliferation and induced apoptosis in gallbladder cancer cells, which suggests that this drug may serve as a novel candidate for adjuvant treatment in patients with gallbladder cancer.

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“…Although there have been advances in chemotherapy, the prognosis of GBC remains poor (6). The mean survival ranges between 5.2 and 24.4 months (7–10). The majority of patients with GBC succumb to metastasis and recurrence following surgery.…”

Section: Introductionmentioning

confidence: 99%

Long non-coding RNA expression profiles in gallbladder carcinoma identified using microarray analysis

et al. 2017

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Gallbladder carcinoma (GBC) is the most common biliary tract cancer and exhibits poor patient prognosis. Previous studies have identified that long non-coding RNAs (lncRNAs) serve important regulatory roles in cancer biology. Alterations in lncRNAs are associated with several types of cancer. However, the contribution of lncRNAs to GBC remains unclear. To investigate the lncRNAs that are potentially involved in GBC, lncRNA profiles were identified in three pairs of human GBC and corresponding peri-carcinomatous tissue samples using microarray analysis. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to validate the microarray data. In order to elucidate potential functions, Gene Ontology, Kyoto Encyclopedia of Genes and Genomes analysis, and network analysis were used to determine relevant signaling pathways. Abundant RNA probes were used, and 1,758 lncRNAs and 1,254 mRNAs were detected to be differentially expressed by the microarray. Compared with para-carcinoma tissue, numerous lncRNAs were markedly upregulated or downregulated in GBC. The results demonstrated that the lncRNAs that were downregulated in GBC were more numerous compared with the lncRNAs that were upregulated. Among them, RP11-152P17.2-006 was the most upregulated, whereas CTA-941F9.9 was the most downregulated. The RT-qPCR results were consistent with the microarray data. Pathway analysis indicated that five pathways corresponded to the differentially expressed transcripts. It was demonstrated that lncRNA expression in GBC was markedly altered, and a series of novel lncRNAs associated with GBC were identified. The results of the present study suggest that the functions of lncRNAs are important in GBC development and progression.

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Outcomes and prognostic factors in gallbladder cancer: a single-centre experience

Cited by 28 publications

References 21 publications

MALAT1 promotes the proliferation and metastasis of gallbladder cancer cells by activating the ERK/MAPK pathway

MALAT1 promotes the proliferation and metastasis of gallbladder cancer cells by activating the ERK/MAPK pathway

Effects of oxymatrine on the apoptosis and proliferation of gallbladder cancer cells

Long non-coding RNA expression profiles in gallbladder carcinoma identified using microarray analysis

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