Ouabain Modulates Zymosan-Induced Peritonitis in Mice

Leite, Jacqueline Alves; Alves, Anne K. de Abreu; Galvão, José Guilherme Marques; Teixeira, Mariana Pires; Cavalcante-Silva, Luiz Henrique Agra; Scavone, Cristóforo; Morrot, Alexandre; Rumjanek, Vivian M.; Rodrigues‐Mascarenhas, Sandra

doi:10.1155/2015/265798

Cited by 28 publications

(50 citation statements)

References 51 publications

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“…Moreover, ouabain exhibited nociceptive actions in the acetic acid-induced writhing response and in the hot plate model. These findings were corroborated and expanded in a study by Leite et al [33], who showed that ouabain (560 µg/kg bodyweight) decreased zymosan-induced peritonitis in mice. The compound reduced fluid extravasation (edema formation), leukocyte infiltration and the levels of the cytokines IL-1β and tumor necrosis factor α (TNFα), but did not influence the viability or function (phagocytosis) of leukocytes.…”

Section: Acute Inflammationsupporting

confidence: 76%

New Knowledge About Old Drugs: The Anti-Inflammatory Properties of Cardiac Glycosides

2017

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In the 19th century, cardio-active steroid glycosides, shortly cardiac glycosides, were scientifically established as drugs against heart failure. Their in vivo, cellular, and molecular actions as well as their predominant target, Na+-K+-ATPase, have been comprehensively investigated in the 20th century and the discovery of endogenous cardiac glycosides has fostered this research field. In the last years, however, results from clinical trials and meta-analyses have questioned their therapeutic value due to efficacy and safety issues. This has led to a considerable decline of their usage. Beyond the cardiovascular system, cardiac glycosides have been increasingly recognized as antitumor compounds and Na+-K+-ATPase has evolved into a promising drug target in oncology. A wealth of review articles exists that intensively discuss these topics. Surprisingly, the anti-inflammatory actions of cardiac glycosides, which were discovered in the 1960s, have so far hardly been perceived and have not yet been summarized. This review provides an overview of the in vivo and in vitro actions of cardiac glycosides on inflammatory processes and of the signaling mechanisms responsible for these effects: cardiac glycosides have been found to decrease inflammatory symptoms in different animal models of acute and chronic inflammation. Regarding the underlying mechanisms most research has focused on leukocytes. In these cells, cardiac glycosides primarily inhibit cell proliferation and the secretion of proinflammatory cytokines.

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Section: Acute Inflammationsupporting

confidence: 76%

New Knowledge About Old Drugs: The Anti-Inflammatory Properties of Cardiac Glycosides

2017

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“…Numerous studies have shown CTS, as natural ligands of NKA, to have modulatory effects on immune cells functioning in vitro and in vivo ( 9 – 11 , 13 , 14 ).…”

Section: Cardiotonic Steroids the Specific Ligands Of Nkamentioning

confidence: 99%

“…Numerous studies have shown CTS to modulate the function of many immune cells, both in vitro and in vivo , with effects via the inhibition of NF-κB and MAPK, thereby controlling some key cellular processes, such as proliferation and differentiation, as well as cytokine production ( 9 – 14 ).…”

Section: Introductionmentioning

confidence: 99%

Cardiotonic Steroids as Modulators of Neuroinflammation

et al. 2016

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Cardiotonic steroids (CTS) are a class of specific ligands of the Na+, K+- ATPase (NKA). NKA is a P-type ATPase that is ubiquitously expressed and although well known to be responsible for the maintenance of the cell electrochemical gradient through active transport, NKA can also act as a signal transducer in the presence of CTS. Inflammation, in addition to importantly driving organism defense and survival mechanisms, can also modulate NKA activity and memory formation, as well as being relevant to many chronic illnesses, neurodegenerative diseases, and mood disorders. The aim of the current review is to highlight the recent advances as to the role of CTS and NKA in inflammatory process, with a particular focus in the central nervous system.

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“…Zymosan, a polysaccharide cell wall component derived from Saccharomyces cerevisiae, is commonly used for the induction of peritonitis and can lead to inflammation [44]. Therefore, it has been widely used as a self-resolving model of inflammation within 30 min of the inflammatory stimulus due to the activation of macrophages and mast cells that release prostaglandins, particularly histamine and leukotrienes [45]. In this study, we used the acetic acid-induced vascular permeability model and zymosan-induced peritonitis model extensively to study the effects of o-VA. Our data demonstrated that o-VA could suppress the migration of leukocyte by inhibiting capillary permeability.…”

Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory Effect of <i>o</i>-Vanillic Acid on Lipopolysaccharide-Stimulated Macrophages and Inflammation Models

Lee¹,

Lee²,

Shin³

et al. 2018

JFNR

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Inflammation is an important biological reaction in the body in response to external stimuli. Excessive inflammation causes various inflammatory disorders such as allergic hypersensitivity, autoimmune disease, rheumatoid arthritis, and cancer. Macrophages play a major role in the inflammatory response by producing inflammatory mediators such as nitric oxide, prostaglandin E 2 , and pro-inflammatory cytokines. Natural products are often a source of bioactive compounds, which have great potential as novel therapeutic agents. Amomum xanthoides extract has been shown to possess various pharmacological activities including anti-inflammatory activity. This study evaluated the anti-inflammatory potential of o-vanillic acid (o-VA), a major compound in A. xanthoides, using lipopolysaccharide (LPS)-induced macrophages and in vivo animal models. o-VA decreased, in a concentration-dependent manner, the LPS-induced gene expression and production of inflammatory mediators, such as inducible nitric oxidase/cyclooxygenase-2 and pro-inflammatory cytokines, by reducing the nuclear factor-κB activation. In addition, o-VA dose-dependently ameliorated acetic acid-induced vascular permeability and zymosan-induced leukocyte migration. Thus, we suggest that o-VA can be used as a pharmacological agent or food supplement in the treatment of inflammatory conditions.

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Ouabain Modulates Zymosan-Induced Peritonitis in Mice

Cited by 28 publications

References 51 publications

New Knowledge About Old Drugs: The Anti-Inflammatory Properties of Cardiac Glycosides

New Knowledge About Old Drugs: The Anti-Inflammatory Properties of Cardiac Glycosides

Cardiotonic Steroids as Modulators of Neuroinflammation

Anti-Inflammatory Effect of <i>o</i>-Vanillic Acid on Lipopolysaccharide-Stimulated Macrophages and Inflammation Models

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