Osterix‐mCherry Expression Allows for Early Bone Detection in a Calvarial Defect Model

Strecker, Sara; Unterman, Shimon; Charles, Lyndon; Pivovarchick, Dmitry; Maye, Peter; Edelman, Elazer R.; Artzi, Natalie

doi:10.1002/adbi.201900184

Cited by 4 publications

(4 citation statements)

References 58 publications

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“…The BMP release pattern from HA particles shown in this study is thus comparable to that from clinically used collagen carriers, despite large experimental variability regarding carrier materials, protein loading, and release conditions [16,33,[75][76][77][78]. Notably, the currently approved high-dose BMP formulations (3.5 to 12 mg) show an initial burst release held responsible for inflammatory and osteoclastic side effects; therefore, lower doses of applied BMPs (present study) and/or a tuning of this initial release by modifications of the carrier material are highly desirable [16][17][18][19].…”

Section: In Vitro Growth Factor Release From the Ha Particlessupporting

confidence: 60%

Single Application of Low-Dose, Hydroxyapatite-Bound BMP-2 or GDF-5 Induces Long-Term Bone Formation and Biomechanical Stabilization of a Bone Defect in a Senile Sheep Lumbar Osteopenia Model

et al. 2022

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Effects of hydroxyapatite (HA) particles with bone morphogenetic BMP-2 or GDF-5 were compared in sheep lumbar osteopenia; in vitro release in phosphate-buffered saline (PBS) or sheep serum was assessed by ELISA. Lumbar (L) vertebral bone defects (Ø 3.5 mm) were generated in aged, osteopenic female sheep (n = 72; 9.00 ± 0.11 years; mean ± SEM). Treatment was: (a) HA particles (2.5 mg; L5); or (b) particles coated with BMP-2 (1 µg; 10 µg) or GDF-5 (5 µg; 50 µg; L4; all groups n = 6). Untouched vertebrae (L3) served as controls. Three and nine months post-therapy, bone formation was assessed by osteodensitometry, histomorphometry, and biomechanical testing. Cumulative 14-day BMP release was high in serum (76–100%), but max. 1.4% in PBS. In vivo induction of bone formation by HA particles with either growth factor was shown by: (i) significantly increased bone volume, trabecular and cortical thickness (overall increase HA + BMP vs. control close to the injection channel 71%, 110%, and 37%, respectively); (ii) partial significant effects for bone mineral density, bone formation, and compressive strength (increase 17%; 9 months; GDF‑5). Treatment effects were not dose-dependent. Combined HA and BMPs (single low-dose) highly augment long-term bone formation and biomechanical stabilization in sheep lumbar osteopenia. Thus, carrier-bound BMP doses 20,000-fold to 1000-fold lower than previously applied appear suitable for spinal fusion/bone regeneration and improved treatment safety.

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Section: In Vitro Growth Factor Release From the Ha Particlessupporting

confidence: 60%

Single Application of Low-Dose, Hydroxyapatite-Bound BMP-2 or GDF-5 Induces Long-Term Bone Formation and Biomechanical Stabilization of a Bone Defect in a Senile Sheep Lumbar Osteopenia Model

et al. 2022

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“…In 2019, Strecker, S. E., and colleagues [ 22 ] investigated the effectiveness rhBMP-2 and a nanocomposite scaffold material in promoting bone tissue regeneration. The immunostaining results indicate a significant increase in the cellular presence of Osterix and Osteocalcin.…”

Section: Resultsmentioning

confidence: 99%

“…Notably, however, the defect site had yet to undergo complete regeneration. This study's results indicate that using Chi-AHP with rhBMP-2 therapy results in superior bone regeneration outcomes when compared to using Chi-AHP alone.In 2019, Strecker, S. E., and colleagues[22] investigated the effectiveness rhBMP-2 and a nanocomposite scaffold material in promoting bone tissue regeneration. The immunostaining results indicate a significant increase in the cellular presence of Osterix and Osteocalcin.…”

mentioning

confidence: 99%

Bone Morphogenic Protein and Mesenchymal Stem Cells to Regenerate Bone in Calvarial Defects: A Systematic Review

Torres‐Guzman

Ávila

Maita

et al. 2023

JCM

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Background: The use of bone morphogenic protein and mesenchymal stem cells has shown promise in promoting bone regeneration in calvarial defects. However, a systematic review of the available literature is needed to evaluate the efficacy of this approach. Methods: We comprehensively searched electronic databases using MeSH terms related to skull defects, bone marrow mesenchymal stem cells, and bone morphogenic proteins. Eligible studies included animal studies that used BMP therapy and mesenchymal stem cells to promote bone regeneration in calvarial defects. Reviews, conference articles, book chapters, and non-English language studies were excluded. Two independent investigators conducted the search and data extraction. Results: Twenty-three studies published between 2010 and 2022 met our inclusion criteria after a full-text review of the forty-five records found in the search. Eight of the 23 studies used mice as models, while 15 used rats. The most common mesenchymal stem cell was bone marrow-derived, followed by adipose-derived. BMP-2 was the most popular. Stem cells were embedded in Scaffold (13), Transduction (7), and Transfection (3), and they were delivered BMP to cells. Each treatment used 2 × 104–1 × 107 mesenchymal stem cells, averaging 2.26 × 106. Most BMP-transduced MSC studies used lentivirus. Conclusions: This systematic review examined BMP and MSC synergy in biomaterial scaffolds or alone. BMP therapy and mesenchymal stem cells in calvarial defects, alone, or with a scaffold regenerated bone. This method treats skull defects in clinical trials. The best scaffold material, therapeutic dosage, administration method, and long-term side effects need further study.

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“…ALP, Runx2, Osterix, and OCN are the most commonly studied osteogenic markers during different stages of bone formation. Both Runx2 and Osterix are osteogenic markers with a well-characterized role in the early stage of bone formation (Strecker et al, 2019;Ou and Huang, 2021). OCN, which is involved in ECM deposition, is regarded as a late marker of osteoblast differentiation (Martins et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Hydrothermal Synthesis of Zinc-Incorporated Nano-Cluster Structure on Titanium Surface to Promote Osteogenic Differentiation of Osteoblasts and hMSCs

Tang

Liu

et al. 2021

Front. Mater.

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In this study, a novel modification strategy was established to synthesize a zinc-incorporated nano-cluster structure on titanium surface in a two-step hydrothermal reaction, and the osteogenic differentiation of osteoblasts and human bone marrow mesenchymal cells (hMSCs) was studied in the presence of this synthesized nanostructure. Analyses of the surface topography and elemental composition revealed that the zinc-containing cluster-like nanostructure was successfully prepared on the titanium surface. By altering the reaction time, three surface modifications were established. The three modified titanium surfaces had improved hydrophilicity and could continuously release zinc ions in a controlled manner. In vitro study displayed that three modified titanium surfaces, especially the samples prepared by reacting for 15 min, exhibited enhanced cell adhesion, proliferation, and osteogenic differentiation compared to the pure titanium surface. The study therefore conclude that the zinc-incorporated nano-cluster modification of titanium surface through a simple procedure can establish an enhanced osteogenic microenvironment and exhibit a potential strategy of titanium surface modification to accelerate the dental implant osseointegration.

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Osterix‐mCherry Expression Allows for Early Bone Detection in a Calvarial Defect Model

Cited by 4 publications

References 58 publications

Single Application of Low-Dose, Hydroxyapatite-Bound BMP-2 or GDF-5 Induces Long-Term Bone Formation and Biomechanical Stabilization of a Bone Defect in a Senile Sheep Lumbar Osteopenia Model

Single Application of Low-Dose, Hydroxyapatite-Bound BMP-2 or GDF-5 Induces Long-Term Bone Formation and Biomechanical Stabilization of a Bone Defect in a Senile Sheep Lumbar Osteopenia Model

Bone Morphogenic Protein and Mesenchymal Stem Cells to Regenerate Bone in Calvarial Defects: A Systematic Review

Hydrothermal Synthesis of Zinc-Incorporated Nano-Cluster Structure on Titanium Surface to Promote Osteogenic Differentiation of Osteoblasts and hMSCs

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