Osteogenic potential of rhBMP9 combined with a bovine‐derived natural bone mineral scaffold compared to rhBMP2

Fujioka‐Kobayashi, Masako; Sawada, Kosaku; Kobayashi, Eizaburo; Benoit, Sandrine; Zhang, Yufeng; Miron, Richard J.

doi:10.1111/clr.12804

Cited by 43 publications

(59 citation statements)

References 43 publications

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“…We have previously shown that ABBM scaffolds are able to facilitate an increase in cell numbers when compared to deproteinized bovine bone mineral that do not contain any collagen or growth factors [18]. Furthermore, collagen type 1 is known to inhibit bacterial pathogens that are often found in the oral cavity such as Staphylococcus aureus, Staphylococcus epidermidis, hemolytic Streptococcus, and Pseudomonas aeruginosa with some studies a decrease of bacterial activity by roughly 40% [19].…”

Section: Discussionmentioning

confidence: 99%

Novel Natural Bovine Bone Graft with Integrated Atelo-Collagen Type I: Atelocollagen Bovine Bone Mineral (ABBM) Characterization In-vivo

Raouf¹,

Zhang²,

Caballé‐Serrano³

et al. 2017

Periodon Prosthodon

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Bone grafting materials are routinely utilized in the fields of dental and orthopedic medicine to augment lost or missing bone. While autogenous bone has been considered the gold standard for many years, a variety of alternatives have been proposed including allografts, xenografts and alloplasts to prevent the additional morbidity and limited supply for autografts. Xenografts have frequently been utilized in many countries due to their supporting osteoconductive potential as well as that in many countries, allografts are not permitted.While typically all collagen and growth factors are removed during the processing of xenografts, recently the development of a natural bone mineral containing atelocollagen type I have been proposed utilizing atelopeptidation and lyophilization technologies modifying the collagen components of bone material within bone structure to non-immunogenic atelocollagen. This relatively novel processing technique does not use heat (thermal) processing when manufacturing which has been shown to negatively impact the natural crystalline micro-structure of hydroxyapatite, causing ceramization and destroying collagen components.The lyophilization technique, involves the evaporation of water contained in a product by sublimation -a previously frozen material is placed in a vacuum, which turns the ice directly to vapor. This new processing technique for xenografts has been shown to preserve lyophilized collagen with lower humidity making the bone matrix hydrophilic. These bone grafts contain roughly 2% moisture, 65-75% hydroxyapatite, 25-35% atelo-collagen content and up to 0.1% non-collagenous proteins. Here we investigated these novel atelo-collagenized bovine bone mineral (ABBM) bone grafts using scanning electron microscopy (SEM) and evaluated their bone regenerative potential in a rat femur animal model.

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Section: Discussionmentioning

confidence: 99%

Novel Natural Bovine Bone Graft with Integrated Atelo-Collagen Type I: Atelocollagen Bovine Bone Mineral (ABBM) Characterization In-vivo

Raouf¹,

Zhang²,

Caballé‐Serrano³

et al. 2017

Periodon Prosthodon

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“…Recently, our group compared the osteogenic potential of rhBMP9 to rhBMP2 and found that rhBMP9 markedly and significantly induced higher osteoblast differentiation as assessed by ALP staining, alizarin red staining and real-time PCR for osteoblast differentiation markers [12,13]. Furthermore, we observed that rhBMP9 even at low concentrations of 10 ng/mL was able to significantly induce over a two-fold increase in osteoblast differentiation when compared to rhBMP2 at a high concentration of 100 ng/mL, representing higher osteogenic potential even at 10-times-lower doses [12,13].…”

Section: Discussionmentioning

confidence: 99%

“…After 14 days, cells were fixed in 96% ethanol for 15 minutes and stained with 0.2% alizarin red (Alizarin red S; Sigma) solution in water (pH 6.4) at room temperature for 1 h as previously described [13]. Alizarin red quantification was performed using images captured on a Nikon D610 camera with a Heerbrugg M400 ZOOM microscope (Wild Heerbrugg).…”

Section: Methodsmentioning

confidence: 99%

“…One of the drawbacks of these few studies characterizing BMP9 were that they were only performed utilizing adenovirus transfections (gene therapy) with no information regarding its recombinant protein activity [5,6,10,11]. Recently our research group investigated for the first time the regenerative potential of a clinically viable recombinant source of human (rh)BMP9 [12,13]. In two separate studies, it was found that rhBMP9 demonstrated up to 10 times more osteopromotion in in vitro osteoblast differentiation when compared to rhBMP2 [12,13].…”

Section: Introductionmentioning

confidence: 99%

“…Recently our research group investigated for the first time the regenerative potential of a clinically viable recombinant source of human (rh)BMP9 [12,13]. In two separate studies, it was found that rhBMP9 demonstrated up to 10 times more osteopromotion in in vitro osteoblast differentiation when compared to rhBMP2 [12,13]. …”

Section: Introductionmentioning

confidence: 99%

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Hyaluronic Acid Gel-Based Scaffolds as Potential Carrier for Growth Factors: An In Vitro Bioassay on Its Osteogenic Potential

Fujioka‐Kobayashi

Benoit

Kobayashi

et al. 2016

JCM

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Hyaluronic acid (HA) has been utilized for a variety of regenerative medical procedures due to its widespread presence in connective tissue and perceived biocompatibility. The aim of the present study was to investigate HA in combination with recombinant human bone morphogenetic protein 9 (rhBMP9), one of the most osteogenic growth factors of the BMP family. HA was first combined with rhBMP9 and assessed for the adsorption and release of rhBMP9 over 10 days by ELISA. Thereafter, ST2 pre-osteoblasts were investigated by comparing (1) control tissue culture plastic, (2) HA alone, and (3) HA with rhBMP9 (100 ng/mL). Cellular proliferation was investigated by a MTS assay at one, three and five days and osteoblast differentiation was investigated by alkaline phosphatase (ALP) activity at seven days, alizarin red staining at 14 days and real-time PCR for osteoblast differentiation markers. The results demonstrated that rhBMP9 adsorbed within HA scaffolds and was released over a 10-day period in a controlled manner. While HA and rhBMP9 had little effect on cell proliferation, a marked and pronounced effect was observed for cell differentiation. rhBMP9 significantly induced ALP activity, mRNA levels of collagen1α2, and ALP and osteocalcin (OCN) at three or 14 days. HA also demonstrated some ability to induce osteoblast differentiation by increasing mRNA levels of OCN and increasing alizarin red staining at 14 days. In conclusion, the results from the present study demonstrate that (1) HA may serve as a potential carrier for various growth factors, and (2) rhBMP9 is a potent and promising inducer of osteoblast differentiation. Future animal studies are now necessary to investigate this combination approach in vivo.

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Membranes for Guided Bone Regeneration: A Road from Bench to Bedside

Aprile

Letourneur

Simón-Yarza

2020

Adv Healthcare Materials

102

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Bone resorption can negatively influence the osseointegration of dental implants. Barrier membranes for guided bone regeneration (GBR) are used to exclude nonosteogenic tissues from influencing the bone healing process. In addition to the existing barrier membranes available on the market, a growing variety of membranes for GBR with tailorable physicochemical properties are under preclinical evaluation. Hence, the aim of this review is to provide a comprehensive description of materials used for GBR and to report the main industrial and regulatory aspects allowing the commercialization of these medical devices (MDs). In particular, a summary of the main attributes defining a GBR membrane is reported along with a description of commercially available and under development membranes. Finally, strategies for the scaling-up of the manufacturing process and the regulatory framework of the main MD producers (USA, EU, Japan, China, and India) are presented. The description of the regulatory approval process of GBR membranes is representative of the typical path that medium-to high-risk MDs have to follow for an effective medical translation, which is of fundamental importance to increase the impact of biomedical research on public health.

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Osteogenic potential of rhBMP9 combined with a bovine‐derived natural bone mineral scaffold compared to rhBMP2

Cited by 43 publications

References 43 publications

Novel Natural Bovine Bone Graft with Integrated Atelo-Collagen Type I: Atelocollagen Bovine Bone Mineral (ABBM) Characterization In-vivo

Novel Natural Bovine Bone Graft with Integrated Atelo-Collagen Type I: Atelocollagen Bovine Bone Mineral (ABBM) Characterization In-vivo

Hyaluronic Acid Gel-Based Scaffolds as Potential Carrier for Growth Factors: An In Vitro Bioassay on Its Osteogenic Potential

Membranes for Guided Bone Regeneration: A Road from Bench to Bedside

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