Osteogenic potential of osteoblasts from neonatal rats born to mothers treated with caffeine throughout pregnancy

Reis, Amanda Maria Sena; Ribeiro, Lorena Gabriela Rocha; Ocarino, Natália de Melo; Góes, Alfredo M.; Serákides, Rogéria

doi:10.1186/s12891-015-0467-8

Cited by 16 publications

(11 citation statements)

References 70 publications

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“…TGF-β1 enhances bone regeneration (Farea et al, 2014) and promotes the synthesis of type I collagen from the osteoblasts. Type I collagen is responsible for bone stability and cell biological functions (Reis et al, 2015). It indicates that Rb1 promotes bone stability and cell biological functions.…”

Section: Discussionmentioning

confidence: 99%

Ginsenoside Rb1 alleviates aluminum chloride-induced rat osteoblasts dysfunction

Zhu

Zheng

et al. 2016

Toxicology

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Osteoblasts dysfunction, induced by aluminum (Al), plays a critical role in the osteoporosis etiology. Ginsenoside Rb1 (Rb1) has the therapeutic properties for osteoporosis. This study aimed to assess the efficiency of Rb1 in ameliorating Al-induced osteoblasts dysfunction. The osteoblasts were divided into four groups: Rb1-treated group (RG, 0.0145mg/mL Rb1), control group (CG, 0), AlCl-treated group (AG, 0.126mg/mL AlCl·6HO), AlCl+Rb1-treated group (ARG, 0.0145mg/mL Rb1 and 0.126mg/mL AlCl·6HO). After 24h of culture, the osteoblasts viability, the transforming growth factor-β1 (TGF-), bone morphogenetic protein-2 (BMP-2), the insulin-like growth factor I (IGF-I), core-binding factor α1 (Cbfα1) mRNA expressions, glutathione perioxidase (GSH-P and superoxide dismutase (SOD) activities, and reactive oxygen species (ROS) concentration were determined. The osteoblasts ultrastructural features were also observed. In the ARG, the osteoblasts viability, TGF-, BMP-2, IGF-I and Cbfα1 mRNA expressions and the GSH-P and SOD activities were significantly increased, the ROS concentration was significantly decreased, and osteoblasts histology lesion was attenuated compared with the AG. These results demonstrated that Rb1 could significantly reverse osteoblasts viability and osteoblasts growth regulation factor, inhibit oxidative stress, and attenuate histology lesion in the osteoblasts with AlCl. These results indicate that Rb1 can effectively alleviate the AlCl-induced osteoblasts dysfunction.

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Section: Discussionmentioning

confidence: 99%

Ginsenoside Rb1 alleviates aluminum chloride-induced rat osteoblasts dysfunction

Zhu

Zheng

et al. 2016

Toxicology

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“…Complementary to the reported viability, proliferation and ALP activity data, the differentiation level of BM-MSCs cultured onto PLLA and PLLA-BBG-Sr membranes was assessed by quantitative PCR of selected bone-specific gene transcripts. In the literature it is well described that osteogenic differentiation of BM-MSCs has three phases: the proliferative phase, which is followed by the ECM synthesis and maturation, and lastly the mineralisation phase [51,52]. Therefore, in this study the osteogenic potential of the cells was evaluated at day 14 and 21 using the expression pattern of the representative osteogenic markers: Alpl, Spp1, Bglap and Sp7 [8,53].…”

Section: Osteogenic Differentiation Markersmentioning

confidence: 99%

Reinforcement of poly-l-lactic acid electrospun membranes with strontium borosilicate bioactive glasses for bone tissue engineering

et al. 2016

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Herein, for the first time, we combined poly-L-lactic acid (PLLA) with a strontium borosilicate bioactive glass (BBG-Sr) using electrospinning to fabricate a composite bioactive PLLA membrane loaded with 10% (w/w) of BBG-Sr glass particles (PLLA-BBG-Sr). The composites were characterised by scanning electron microscopy (SEM) and microcomputer tomography (l-CT), and the results showed that we successfully fabricated smooth and uniform fibres (1-3 lm in width) with a homogeneous distribution of BBG-Sr microparticles (<45 lm). Degradation studies (in phosphate buffered saline) demonstrated that the incorporation of BBG-Sr glass particles into the PLLA membranes increased their degradability and water uptake with a continuous release of cations. The addition of BBG-Sr glass particles enhanced the membrane's mechanical properties (69% higher Young modulus and 36% higher tensile strength). Furthermore, cellular in vitro evaluation using bone marrow-derived mesenchymal stem cells (BM-MSCs) demonstrated that PLLA-BBG-Sr membranes promoted the osteogenic differentiation of the cells as demonstrated by increased alkaline phosphatase activity and up-regulated osteogenic gene expression (Alpl, Sp7 and Bglap) in relation to PLLA alone. These results strongly suggest that the composite PLLA membranes reinforced with the BBG-Sr glass particles have potential as an effective biomaterial capable of promoting bone regeneration. Statement of Significance PLLA membranes were reinforced with 10% (w/w) of strontium-bioactive borosilicate glass microparticles, and their capacity to induce the osteogenic differentiation of bone marrow mesenchymal stem cells (BM-MSCs) was evaluated. These membranes presented an increased: degradability, water uptake, Young modulus and tensile strength. We also demonstrated that these membranes are non-cytotoxic and promote the attachment of BM-MSCs. The addition of the glass microparticles into the PLLA membranes promoted the increase of ALP activity (under osteogenic conditions), as well as the BM-MSCs osteogenic differentiation as shown by the upregulation of Alpl, Sp7 and Bglap gene expression. Overall, we demonstrated that the reinforcement of PLLA with glass microparticles results in a biomaterial with the appropriate properties for the regeneration of bone tissue.

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“…7 Caffeine binds to adenosine receptors and modulates several other receptors including: glucocorticoid, insulin, estrogen, androgens, vitamin D, cannabinoids, glutamate and adrenergic receptors, all of which are expressed in osteoblasts or osteoprogenitor cells and have important functions during osteoblast differentiation. 29 Moreover, Vitamin D will promote greater transcription of RANKL and limit the production of osteoprotegerin (OPG). 30 Previous research has argued that, while low caffeine concentration (0.005-0.01 mM) cannot affect cell survival and osteoblast differentiation from bone marrow mesenchymal stem cells (MSC), it can significantly increase both osteoclast differentiation from bone marrow hematopoietic stem cells (HSCs) and bone resorption activity.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of orthodontic tooth movement by 3D micro-computed tomography (µ-CT) following caffeine administration

et al. 2019

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Background: The compressive strength of orthodontic tooth movement will be distributed throughout the periodontal ligament and alveolar bone, resulting in bone resorption on the pressure side and new bone formation on the tension side. Caffeine, a member of the methyl xanthine family, represents a widely-consumed psychoactive substance that can stimulate osteoclastogenesis through an increase in RANKL. A 3D Micro-Computed Tomography (µ-CT) x-ray device can be used to measure orthodontic tooth movement and changes in periodontal ligament width. Purpose: The purpose of this research was to analyze the effects of caffeine on the distal movement distance of two mandibular incisors using 3D µ-CT. Methods: The research subjects (guinea pigs) were randomly divided into four groups. Of the two control groups created, one received two weeks of treatment and the other three weeks. The members of these two control groups were subjected to orthodontic movement but received no caffeine. Meanwhile, the other two groups were treatment groups whose members also received either two or three weeks of treatment. In these two treatment groups, the subjects were subjected to orthodontic movement and received a 6 mg/500 BM dose of caffeine. The orthodontic movement of the subjects was induced by installing a band matrix and orthodontic bracket on each mandibular incisor to move distally by means of an open coil spring. Observations were then conducted on days 15 and 22 with µ-CT x-rays to measure the distal movement distance of the two mandibular incisors and the width of the periodontal ligament. Results: The administration of caffeine increased the tooth movement on day 15 (p<0.05) and day 22 (p<0.05). The increase in the tooth movement on day 22 was greater than that on day 15 (p<0.05). The width of the periodontal ligament on the pressure side of the treatment groups experienced greater narrowing than that of the control groups (p<0.05). Meanwhile, the width of periodontal ligament on the tension side of the treatment groups widened more than that of the control groups (p<0.05). Conclusion: µ-CT x-ray can be used to evaluate the extent of orthodontic movement in addition to the width of the mandibular incisor periodontal ligament during orthodontic tooth movement. Moreover, it has been established that the administering of caffeine can improve orthodontic tooth movement.

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Osteogenic potential of osteoblasts from neonatal rats born to mothers treated with caffeine throughout pregnancy

Cited by 16 publications

References 70 publications

Ginsenoside Rb1 alleviates aluminum chloride-induced rat osteoblasts dysfunction

Ginsenoside Rb1 alleviates aluminum chloride-induced rat osteoblasts dysfunction

Reinforcement of poly-l-lactic acid electrospun membranes with strontium borosilicate bioactive glasses for bone tissue engineering

Evaluation of orthodontic tooth movement by 3D micro-computed tomography (µ-CT) following caffeine administration

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