Osteogenic peptides in periodontal ligament stem cell-containing three-dimensional bioscaffolds promote bone healing

Cai, Meili; Liu, Yaoyao; Tian, Yinping; Liang, Yan; Xu, Zinan; Liu, Fangchen; Lai, Renfa; Zhou, Zhen; Liu, Minyi; Dai, Jian; Liu, Xiangning

doi:10.1039/d1bm01673c

Cited by 3 publications

(4 citation statements)

References 47 publications

(57 reference statements)

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“…Therefore, researchers have considered using three-dimensional scaffolds to provide a more suitable environment for osteogenic differentiation of PDLSCs, enabling them to exert their osteogenic potential in other regions. Cai et al placed PDLSCs in a peptide-containing bioscaffold and observed increased bone formation while reducing chondrocyte generation ( Cai et al, 2022 ). Zhao et al co-cultured PDLSCs with umbilical vein endothelial cells (UVECs) in a calcium phosphate scaffold implanted into a cranial bone defect in a large animal model.…”

Section: Periodontal Ligament Stem Cells and Gingival Mesenchymal Ste...mentioning

confidence: 99%

Mesenchymal stem cells in craniofacial reconstruction: a comprehensive review

Zheng,

Liu,

Liu

et al. 2024

Front. Mol. Biosci.

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Craniofacial reconstruction faces many challenges, including high complexity, strong specificity, severe injury, irregular and complex wounds, and high risk of bleeding. Traditionally, the “gold standard” for treating craniofacial bone defects has been tissue transplantation, which involves the transplantation of bone, cartilage, skin, and other tissues from other parts of the body. However, the shape of craniofacial bone and cartilage structures varies greatly and is distinctly different from ordinary long bones. Craniofacial bones originate from the neural crest, while long bones originate from the mesoderm. These factors contribute to the poor effectiveness of tissue transplantation in repairing craniofacial defects. Autologous mesenchymal stem cell transplantation exhibits excellent pluripotency, low immunogenicity, and minimally invasive properties, and is considered a potential alternative to tissue transplantation for treating craniofacial defects. Researchers have found that both craniofacial-specific mesenchymal stem cells and mesenchymal stem cells from other parts of the body have significant effects on the restoration and reconstruction of craniofacial bones, cartilage, wounds, and adipose tissue. In addition, the continuous development and application of tissue engineering technology provide new ideas for craniofacial repair. With the continuous exploration of mesenchymal stem cells by researchers and the continuous development of tissue engineering technology, the use of autologous mesenchymal stem cell transplantation for craniofacial reconstruction has gradually been accepted and promoted. This article will review the applications of various types of mesenchymal stem cells and related tissue engineering in craniofacial repair and reconstruction.

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Section: Periodontal Ligament Stem Cells and Gingival Mesenchymal Ste...mentioning

confidence: 99%

Mesenchymal stem cells in craniofacial reconstruction: a comprehensive review

Zheng,

Liu,

Liu

et al. 2024

Front. Mol. Biosci.

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Section: In Vivo Advances In the 3d Bioprinting Of Bonementioning

confidence: 99%

“…Among all the biologically active molecules studied in literature, oligopeptides have been proposed as an alternative to growth factors due to their high bioavailability, lower synthesis cost and easier formulation [ 150 , 151 ]. For example, Cai et al [ 150 ]. combined the osteoblast-specific binding oligopeptide SDSSD with human periodontal ligament stem cells (PDLSCs) to develop a 3D bioscaffold and evaluated its physical and biological properties.…”

Section: In Vivo Advances In the 3d Bioprinting Of Bonementioning

confidence: 99%

Integrating bioprinting, cell therapies and drug delivery towards in vivo regeneration of cartilage, bone and osteochondral tissue

Abbadessa,

Ronca,

Salerno

2023

Drug Deliv. and Transl. Res.

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The biological and biomechanical functions of cartilage, bone and osteochondral tissue are naturally orchestrated by a complex crosstalk between zonally dependent cells and extracellular matrix components. In fact, this crosstalk involves biomechanical signals and the release of biochemical cues that direct cell fate and regulate tissue morphogenesis and remodelling in vivo. Three-dimensional bioprinting introduced a paradigm shift in tissue engineering and regenerative medicine, since it allows to mimic native tissue anisotropy introducing compositional and architectural gradients. Moreover, the growing synergy between bioprinting and drug delivery may enable to replicate cell/extracellular matrix reciprocity and dynamics by the careful control of the spatial and temporal patterning of bioactive cues. Although significant advances have been made in this direction, unmet challenges and open research questions persist. These include, among others, the optimization of scaffold zonality and architectural features; the preservation of the bioactivity of loaded active molecules, as well as their spatio-temporal release; the in vitro scaffold maturation prior to implantation; the pros and cons of each animal model and the graft-defect mismatch; and the in vivo non-invasive monitoring of new tissue formation. This work critically reviews these aspects and reveals the state of the art of using three-dimensional bioprinting, and its synergy with drug delivery technologies, to pattern the distribution of cells and/or active molecules in cartilage, bone and osteochondral engineered tissues. Most notably, this work focuses on approaches, technologies and biomaterials that are currently under in vivo investigations, as these give important insights on scaffold performance at the implantation site and its interaction/integration with surrounding tissues. Graphical Abstract

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“…For instance, the SDSSD peptide was immobilized on the zirconia implant surface to enhance osteoblast bioactivity [ 17 ]. Cai Mingxiang et al reported that SDSSD-modified 3D bio scaffolds facilitate osteogenesis and bone formation in the subcutaneous pocket of BALB/c nude mice and facilitate bone healing in vivo [ 18 ]. The author has also reported that bone formation was promoted by binding SDSSD to the G protein-coupled receptor and regulating the AKT signaling pathway.…”

Section: Introductionmentioning

confidence: 99%

Peptide Engraftment on PEGylated Nanoliposomes for Bone Specific Delivery of PTH (1-34) in Osteoporosis

et al. 2023

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Bone-specific functionalization strategies on liposomes are promising approaches to delivering the drug in osteoporotic conditions. This approach delivers the drug to the bone surface specifically, reduces the dose and off-target effects of the drug, and thereby reduces the toxicity of the drug. The purpose of the current research work was to fabricate the bone-specific peptide conjugated pegylated nanoliposomes to deliver anabolic drug and its physicochemical evaluations. For this, a bone-specific peptide (SDSSD) was synthesized, and the synthesized peptide was conjugated with a linker (DSPE-PEG2000-COOH) to obtain a bone-specific conjugate (SDSSD-DSPE). Purified SDSSD-DSPE was characterized by HPLC, Maldi-TOF, NMR, and Scanning Electron Microscope/Energy Dispersive Spectroscopy (SEM/EDS). Further, peptide-conjugated and anabolic drug-encapsulated liposomes (SDSSD-LPs) were developed using the ethanol injection method and optimized by Central Composite Design (CCD) using a statistical approach. Optimized SDSSD-LPs were evaluated for their physicochemical properties, including surface morphology, particle size, zeta potential, in vitro drug release, and bone mineral binding potential. The obtained results from these studies demonstrated that SDSSD-DSPE conjugate and SDSSD-LPs were optimized successfully. The particle size, % EE, and zeta potential of SDSSD-LPs were observed to be 183.07 ± 0.85 nm, 66.72 ± 4.22%, and −25.03 ± 0.21 mV, respectively. SDSSD-LPs demonstrated a sustained drug release profile. Further, the in vitro bone mineral binding assay demonstrated that SDSSD-LPs deliver the drug to the bone surface specifically. These results suggested that SDSSD-LPs could be a potential targeting approach to deliver the anabolic drug in osteoporotic conditions.

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Osteogenic peptides in periodontal ligament stem cell-containing three-dimensional bioscaffolds promote bone healing

Abstract: The oligopeptide SDSSD promotes the osteogenic differentiation of human periodontal ligament stem cells. The 3D bioscaffolds with SDSSD enhance bone formation and the repair effect of bone defects in mice by regulating the AKT signaling pathway.

Cited by 3 publications

References 47 publications

Mesenchymal stem cells in craniofacial reconstruction: a comprehensive review

Mesenchymal stem cells in craniofacial reconstruction: a comprehensive review

Integrating bioprinting, cell therapies and drug delivery towards in vivo regeneration of cartilage, bone and osteochondral tissue

Peptide Engraftment on PEGylated Nanoliposomes for Bone Specific Delivery of PTH (1-34) in Osteoporosis

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