Osteogenic cell response to calcium aluminate‐based cement

Abstract

CAC+ supported the acquisition of the osteogenic cell phenotype in vitro, rendering this novel material a potential alternative to MTA in endodontic procedures. Further in vivo studies are needed to verify if the beneficial in vitro effects of CAC+ on osteoblastic cells correspond to an increase and/or acceleration of bone repair in the periapical region.

Calcium chloride‐enriched calcium aluminate cement promotes in vitro osteogenesis

“…, Castro‐Raucci et al . , , Oliveira et al . ), (ii) CACz+: with 10% CaCl 2 and 25% ZnO, (iii) CACb: with 2.8% of CaCl 2 and 25% Bi 2 O 3 (do Carmo et al .…”

“…After washing in PB, cells were permeabilized with 0.5% Triton X‐100 in PB for 10 min and submitted to epifluorescence protocol as described previously (Castro‐Raucci et al . ) using Alexa Fluor 488‐conjugated phalloidin (green fluorescence, 1:200, Molecular Probes, Invitrogen, Eugene, OR, USA) for actin cytoskeleton labelling and 300 nM 4′,6‐diamidino‐2‐phenylindole, dihydrochloride (blue fluorescence, DAPI, Molecular Probes) for cell nuclei staining. The samples were examined under epifluorescence using an AxioImager M2 Zeiss light microscope (Carl Zeiss Inc., Oberkochen, Germany) outfitted with an AxioCam MRM digital camera (Carl Zeiss Inc.).…”

“…After washing, samples were processed for immunofluorescence labelling (Castro‐Raucci et al . ) using a primary monoclonal antibody to BSP (WVID1‐9C5, 1:200; Developmental Studies Hybridoma Bank‐DSHB, Iowa City, IA, USA) and Alexa Fluor 488 (green fluorescence)‐conjugated goat anti‐mouse secondary antibody (1:200, Molecular Probes). Cell nuclei were stained with DAPI (Molecular Probes).…”

“…) as well as higher gene expression of runt‐related transcription factor 2, osterix, ALP, and bone sialoprotein and osteocalcin, key osteoblast markers (Castro‐Raucci et al . ). However, similar results regarding matrix mineralization of osteoblast cell cultures were observed for MTA and CAC.…”

Improving the Radiopacity of Calcium Aluminate Cement Based Blends

“…Hidroxyapatite-ZnO composites containing 10 wt% or lower ZnO, exhibited the ability to support cell adhesion and their proliferation 40 . Increased cell viability, proliferation and ALP activity were also observed for CAC exposed osteogenic cell culture, which was partially attributed to the Ca 2+ levels released by this cement 41 .…”