Osteocalcin concentrations in plasma prepared with different anticoagulants

Power, Michael; O'Dwyer, Bernadette; Breen, E. G.; Fottrell, P. F.

doi:10.1093/clinchem/37.2.281

Cited by 23 publications

(6 citation statements)

References 14 publications

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“…The samples were thawed immediately before use, and only previously unthawed aliquots were used for the serotonin assay. A previous study from our group (9) has demonstrated the stability of osteocalcin, a relatively unstable protein, (10) under our collection and storage conditions.…”

Section: Sample Collectionmentioning

confidence: 86%

Relation of serum serotonin levels to bone density and structural parameters in women

Mödder

Achenbach

Amin

et al. 2010

Journal of Bone and Mineral Research

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Recent studies have demonstrated an important role for circulating serotonin in regulating bone mass in rodents. In addition, patients treated with selective serotonin reuptake inhibitors (SSRIs) have reduced areal bone mineral density (aBMD). However, the potential physiologic role of serotonin in regulating bone mass in humans remains unclear. Thus we measured serum serotonin levels in a population-based sample of 275 women and related these to total-body and spine aBMD assessed by dual-energy X-ray absorptiometry, femur neck total and trabecular volumetric BMD (vBMD) and vertebral trabecular vBMD assessed by quantitative computed tomography (QCT), and bone microstructural parameters at the distal radius assessed by high-resolution peripheral QCT (HRpQCT). Serotonin levels were inversely associated with body and spine aBMD (age-adjusted R = −0.17 and −0.16, P < .01, respectively) and with femur neck total and trabecular vBMD (age-adjusted R = −0.17 and −0.25, P < .01 and < .001, respectively) but not lumbar spine vBMD. Bone volume/tissue volume, trabecular number, and trabecular thickness at the radius were inversely associated with serotonin levels (age-adjusted R = −0.16, −0.16, and −0.14, P < .05, respectively). Serotonin levels also were inversely associated with body mass index (BMI; age-adjusted R = −0.23, P < .001). Multivariable models showed that serotonin levels remained significant negative predictors of femur neck total and trabecular vBMD, as well as trabecular thickness at the radius, after adjusting for age and BMI. Collectively, our data provide support for a physiologic role for circulating serotonin in regulating bone mass in humans. © 2010 American Society for Bone and Mineral Research

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Section: Sample Collectionmentioning

confidence: 86%

Relation of serum serotonin levels to bone density and structural parameters in women

Mödder

Achenbach

Amin

et al. 2010

Journal of Bone and Mineral Research

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“…This marker is also relatively unstable; its immunoreactivity reportedly decreases by 50–70% after 6–24 hours at room temperature and 40–80% after 2 weeks at 4℃ 2021. Repeated freezethaw cycles also reduce osteocalcin values 22. Moreover, the restricted times during which blood samples could be acquired introduce the possibility of diurnal variations.…”

Section: Discussionmentioning

confidence: 99%

Serum Procollagen Type I N-Terminal Propeptide and Osteocalcin Levels in Korean Children and Adolescents

et al. 2019

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PurposeBone markers can be useful for the diagnosis and treatment of skeletal diseases in children and adolescents. Owing to high skeletal growth velocity and rapid bone turnover, children and adolescents have higher bone marker levels than adults. Thus, a valid age- and sex-specific reference should be established for pediatric populations living in similar environments. We aimed to assess the associations of procollagen type I N-terminal propeptide (P1NP) and osteocalcin with age and sex in a group of healthy Korean children and adolescents.Materials and MethodsThe participants (290 boys and 290 girls, age range 0–18 years) were Korean outpatients. Serum P1NP and osteocalcin levels were measured in control materials and patient samples by electrochemiluminescence immunoassay using an automated Cobas e411 analyzer.ResultsSignificant age-dependent variations in bone marker levels were observed in both sexes (p<0.001). The highest P1NP levels were observed during the first year of life; thereafter, levels decreased until puberty. There was no postnatal peak for osteocalcin; however, its levels remained higher than the adult reference range throughout childhood. Significant differences were observed between boys and girls (p<0.05), especially between the ages of 12 and 17 years. Cobas e411 results for P1NP showed satisfactory precision and linearity.ConclusionWe established reference data for P1NP and osteocalcin levels in healthy Korean children and adolescents, as the first and only study of these parameters in pre-adulthood in Korea. Cobas e411-quantified bone markers may be useful for determining bone metabolism indices.

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“…Osteocalcin (OC) was measured using an ELISA ( enzyme-linked immunoabsorbent assay ) kit (DIAsource ImmunoAssays S.A., Belgium) [ 23 , 24 ]; the normal reference range is 5–25 ng/mL, with an intra-assay variation of 3–5% and an inter-assay variation of 3.5–5.6%. C-terminal telopeptide (CTX) was measured using an ELISA kit (Immuno Diagnostic Systems Ltd, United Kingdom) [ 25 ]; the normal reference range for postmenopausal women is 0.142–1.351 ng/mL, with an intra-assay variation of 1.7–3% and inter-assay variation of 3–11%.…”

Section: Methodsmentioning

confidence: 99%

The role of enteric hormone GLP-2 in the response of bone markers to a mixed meal in postmenopausal women with type 2 diabetes mellitus

Lopes

Schwartz

Ferraz-de-Souza

et al. 2015

Diabetol Metab Syndr

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BackgroundType 2 diabetes mellitus (T2D) is a complex disease associated with several chronic complications, including bone fragility and high fracture risk due to mechanisms not yet fully understood. The influence of the gastrointestinal tract and its hormones on bone remodeling has been demonstrated in healthy individuals. Glucagon-like peptide 2 (GLP-2), an enteric hormone secreted in response to nutrient intake, has been implicated as a mediator of nutrient effects on bone remodeling. This study aimed to analyze the dynamics of bone resorption marker C-terminal telopeptide of type I collagen (CTX), bone formation marker osteocalcin, and GLP-2 in response to a mixed meal in diabetic postmenopausal women.MethodsForty-three postmenopausal women with osteopenia or osteoporosis (20 controls – group CO – and 23 diabetic – group T2D) were subjected to a standard mixed meal tolerance test, with determination of serum CTX, plasma osteocalcin and serum GLP-2 concentrations at baseline and 30, 60, 120 and 180 minutes after the meal.ResultsT2D women had higher body mass index as well as higher femoral neck and total hip bone mineral density. At baseline, luteinizing hormone, follicle-stimulating hormone, osteocalcin and CTX levels were lower in group T2D. In response to the mixed meal, CTX and osteocalcin levels decreased and GLP-2 levels increased in both groups. The expected CTX suppression in response to the mixed meal was lower in group T2D.ConclusionsBone turnover markers were significantly reduced in T2D women at baseline. Confirming the role of nutrient intake as a stimulating factor, GLP-2 increased in response to the mixed meal in both groups. Importantly, CTX variation in response to the mixed meal was reduced in T2D women, suggesting abnormal response of bone remodeling to nutrient intake in T2D.

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Osteocalcin concentrations in plasma prepared with different anticoagulants

Cited by 23 publications

References 14 publications

Relation of serum serotonin levels to bone density and structural parameters in women

Relation of serum serotonin levels to bone density and structural parameters in women

Serum Procollagen Type I N-Terminal Propeptide and Osteocalcin Levels in Korean Children and Adolescents

The role of enteric hormone GLP-2 in the response of bone markers to a mixed meal in postmenopausal women with type 2 diabetes mellitus

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