Osteocalcin and matrix Gla protein in zebrafish (Danio rerio) and Senegal sole (Solea senegalensis): Comparative gene and protein expression during larval development through adulthood

Gavaia, Paulo J.; Simes, Dina C.; Ortiz-Delgado, Juan B.; Viegas, Carla S. B.; Pinto, Jorge P.; Kelsh, Robert N.; Sarasquete, Carmen; Cancela, M. Leonor

doi:10.1016/j.modgep.2005.11.010

Cited by 85 publications

(91 citation statements)

References 51 publications

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“…Vertebrae and kidney, in contrast with heart and branchial arches, showed very low levels of mgp mRNA by qPCR although expression in these tissues was confirmed by in situ hybridization. In vertebrae, mgp expression was associated mainly to the proliferating and differentiated chondrocytes from the intervertebral cartilage, in agreement with previous reports (Gavaia et al, 2006;Pinto et al, 2003), and to the notochord, in particular the chordoblasts.…”

Section: The Highest Levels Of Turbot Mgp Gene Expression Are Found Isupporting

confidence: 92%

“…This could be related to Mgp proposed role in maintenance of a non calcified cartilage matrix, but also as a possible mediator of calcium metabolism occurring at the gill level. Accordingly, a strong mgp expression associated with these structures was previously observed in other fish species such as gilthead seabream (S. aurata), zebrafish (Danio rerio) and Senegalese sole (Solea senegalensis) (Gavaia et al, 2006;Pinto et al, 2003). Furthermore, the purification of sizeable amounts of Mgp from fish was only possible using calcified branchial arches (Simes et al, 2003) confirming the high content of Mgp in these structures, probably due to its high affinity for calcified matrices.…”

Section: The Highest Levels Of Turbot Mgp Gene Expression Are Found Isupporting

confidence: 62%

“…Our results by in situ hybridization confirmed that cardiomyocytes were expressing mgp. Furthermore, localization of mgp mRNA in cardiac structures by in situ hybridization was also previously shown to occur in fish by our group (Gavaia et al, 2006;Ortiz-Delgado et al, 2006;Pinto et al, 2003). The presence of high levels of mgp in heart tissue is indicative of its function as a calcification inhibitor since this tissue has been shown to be a target of ectopic calcifications during pathological conditions, where expression and/or function of mgp is altered (Jono et al, 2006;Luo et al, 1997;Proudfoot and Shanahan, 2006;Schurgers et al, 2005).…”

Section: The Highest Levels Of Turbot Mgp Gene Expression Are Found Isupporting

confidence: 57%

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Matrix Gla protein in turbot (Scophthalmus maximus): Gene expression analysis and identification of sites of protein accumulation

et al. 2009

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Matrix Gla protein (Mgp) is a secreted vitamin K-dependent extracellular matrix protein and a physiological inhibitor of calcification whose gene structure, amino acid sequence and tissue distribution have been conserved throughout evolution. In the present work, the turbot (Scophthalmus maximus) mgp cDNA was cloned and the sequence of the deduced protein compared to that of other vertebrates. As expected, it was closer to teleosts than to other vertebrate groups but there was a strict conservation of amino-acids thought to be important for protein function. Analysis of mgp gene expression indicated branchial arches as the site with higher levels of expression, followed by heart, vertebra and kidney. These results were confirmed by in situ hybridization with a strong mgp expression in branchial arch chondrocytes. Mgp was found to accumulate in gills where it appeared to be restricted to chondrocytes from branchial filaments, while in vertebrae it was localized in vertebral end plates, in growth zones, in vertebral arches and spines and in notochord cells. In the soft tissues analysed, Mgp was mainly detected in kidney and heart, consistent with previous data and providing further evidence for a role of Mgp as a calcification inhibitor and a modulator of the mineralization process. Our studies provide evidence that turbot, an important new species for aquaculture, is also a useful model to study function and expression of Mgp.

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Section: The Highest Levels Of Turbot Mgp Gene Expression Are Found Isupporting

confidence: 92%

Section: The Highest Levels Of Turbot Mgp Gene Expression Are Found Isupporting

confidence: 62%

Section: The Highest Levels Of Turbot Mgp Gene Expression Are Found Isupporting

confidence: 57%

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Matrix Gla protein in turbot (Scophthalmus maximus): Gene expression analysis and identification of sites of protein accumulation

et al. 2009

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“…We have chosen the medaka for this analysis, as bone formation is more rapid in this model compared with zebrafish. Appearance of first alizarin red-positive bone matrix in the vertebrae, for example, is detected 5 days after fertilization in medaka (Inohaya et al, 2007), while the same structures become visible only at 9 days in zebrafish (Gavaia et al, 2006). Osterix is a key regulator driving the differentiation of mesenchymal progenitor cells into the osteoblast lineage in mammals (Nakashima et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

“…Twist expression is a marker for mesenchymal cells, which differentiate into several cell types, including adipocytes, chondrocytes, and osteoblasts (Komori, 2006). Osteocalcin, on the other hand, is a marker for mature bone matrix producing cells in mammals as well as teleost species (Aubin and Liu, 1996;Pinto et al, 2003;Gavaia et al, 2006). However, because twist-positive mesenchymal precursor cells still have to complete several intermediate stages of differentiation before becoming active osteoblasts, a gap remains between twist and osteocalcin-positive cells.…”

Section: Introductionmentioning

confidence: 99%

Osterix‐mCherry transgenic medaka for in vivo imaging of bone formation

Renn

Winkler

2008

Developmental Dynamics

111

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Intramembranous and chondral bone formation by osteoblasts is found in all vertebrates. The genetic network controlling osteoblast differentiation is highly conserved and regulated by a small number of key factors, including the zinc-finger transcription factor Osterix. Expression analysis of osterix in the teleost model medaka revealed a highly restricted expression in skeletal regions. For in vivo imaging, we generated transgenic medaka expressing mCherry under control of the osterix promoter. We show that the transgene becomes expressed in early osteoblasts, which have not yet mineralized bone matrix, and remains high in matured and mineralizing osteoblasts. Life imaging of transgenic larvae provided insight into the appearance and behavior of early osteoblasts during development of the teleost cranium, vertebrae, and caudal fin. In summary, osterix-mCherry transgenic medaka enable us to analyze osteoblasts during different maturation phases in vivo and represent a unique tool to study osteoblast behavior in vertebrate embryos and adults. Developmental Dynamics 238:241-248, 2009.

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