OspTAC2 encodes a pentatricopeptide repeat protein and regulates rice chloroplast development

Wang, Dekai; Liu, Heqin; Zhai, Guowei; Wang, Yunlong; Shao, Jianfeng; Tao, Yuezhi

doi:10.1016/j.jgg.2016.09.002

Cited by 37 publications

(36 citation statements)

References 43 publications

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“…Their loss-of-function mutations caused retarded growth (Kim et al, 2009; Toda et al, 2012). In addition to the mitochondrially-localized PPR proteins listed above, previous studies also identified several chloroplast-targeted PPR members, including OsPPR1 (Gothandam et al, 2005), YSA (Su et al, 2012), OsV4 (Gong et al, 2014), WSL (Tan et al, 2014), ASL3 (Lin et al, 2015a), and OspTAC2 (Wang D. et al, 2016). A conspicuous feature of these rice mutants or their corresponding RNAi transgenics is the chlorophyll (Chl) deficient phenotype and abnormally developed chloroplasts at seedling stage, suggesting essential roles in chloroplast development at an early leaf growth stage.…”

Section: Introductionmentioning

confidence: 96%

“…Due to lack of in-depth functional studies on these genes, only WSL has thus far been implicated in RNA splicing of chloroplast transcript rpl2 (Tan et al, 2014). Additionally, OspTAC2 may act as the core subunit of the PEP complex based on studies of the Arabidopsis homolog pTAC2 (Wang D. et al, 2016). Despite significant advances, the roles of the PPR proteins in regulating post-transcriptional modification of organelle genes, especially chloroplast transcripts, remain obscure.…”

Section: Introductionmentioning

confidence: 99%

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WHITE STRIPE LEAF4 Encodes a Novel P-Type PPR Protein Required for Chloroplast Biogenesis during Early Leaf Development

et al. 2017

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Pentatricopeptide repeat (PPR) proteins comprise a large family in higher plants and perform diverse functions in organellar RNA metabolism. Despite the rice genome encodes 477 PRR proteins, the regulatory effects of PRR proteins on chloroplast development remains unknown. In this study, we report the functional characterization of the rice white stripe leaf4 (wsl4) mutant. The wsl4 mutant develops white-striped leaves during early leaf development, characterized by decreased chlorophyll content and malformed chloroplasts. Positional cloning of the WSL4 gene, together with complementation and RNA-interference tests, reveal that it encodes a novel P-family PPR protein with 12 PPR motifs, and is localized to chloroplast nucleoids. Quantitative RT-PCR analyses demonstrate that WSL4 is a low temperature response gene abundantly expressed in young leaves. Further expression analyses show that many nuclear- and plastid-encoded genes in the wsl4 mutant are significantly affected at the RNA and protein levels. Notably, the wsl4 mutant causes defects in the splicing of atpF, ndhA, rpl2, and rps12. Our findings identify WSL4 as a novel P-family PPR protein essential for chloroplast RNA group II intron splicing during early leaf development in rice.

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Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

WHITE STRIPE LEAF4 Encodes a Novel P-Type PPR Protein Required for Chloroplast Biogenesis during Early Leaf Development

et al. 2017

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“…The mitochondrion-localized OsFLO10 is mainly involved in the trans-splicing of the nad1 intron 1 in mitochondria (Wu et al 2019), and the nucleus-localized OsNPPR1 was reported to be involved in the intron splicing of a small number of nuclearlocalized genes, many of which are mitochondrionlocalized (Hao et al 2019). The chloroplast-localized and/or mitochondrion-localized PPR proteins, such as OsPPR1 (Gothandam et al 2005), OsOTP51, OsPPR4, OsWSL, OsALS3 (Lin et al 2015a), OspTAC2 (Wang et al 2016a), OsPPR6 (Tang et al 2017), OsSLA4, OsWSL5 and OsPGL12, are essential for early chloroplast development in rice at the seedling stage.…”

Section: Introductionmentioning

confidence: 99%

OsSLC1 Encodes a Pentatricopeptide Repeat Protein Essential for Early Chloroplast Development and Seedling Survival

Shang

Chen

et al. 2020

Rice

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Background: The large family of pentatricopeptide repeat (PPR) proteins is widely distributed among land plants. Such proteins play vital roles in intron splicing, RNA editing, RNA processing, RNA stability and RNA translation. However, only a small number of PPR genes have been identified in rice. Results: In this study, we raised a mutant from tissue-culture-derived plants of Oryza sativa subsp. japonica 'Zhonghua 11', which exhibited a lethal chlorosis phenotype from germination to the third-leaf stage. The mutant was designated seedling-lethal chlorosis 1 (slc1). The slc1 mutant leaves showed extremely low contents of photosynthetic pigments and abnormal chloroplast development, and were severely defective in photosynthesis. Map-based cloning of OsSLC1 revealed that a single base (G) deletion was detected in the first exon of Os06g0710800 in the slc1 mutant, which caused a premature stop codon. Knockout and complementation experiments further confirmed that OsSLC1 is responsible for the seedling-lethal chlorosis phenotype in the slc1 mutant. OsSLC1 was preferentially expressed in green leaves, and encoded a chloroplast-localized PPR protein harboring 12 PPR motifs. Loss-of-function of OsSLC1 affected the intron splicing of multiple group II introns, and especially precluded the intron splicing of rps16, and resulted in significant increase in the transcript levels of 3 chloroplast ribosomal RNAs and 16 chloroplast development-related and photosynthesis-related genes, and in significant reduction in the transcript levels of 1 chloroplast ribosomal RNAs and 2 chloroplast development-related and photosynthesis-related genes. Conclusion: We characterized a novel chloroplast-localized PPR protein, OsSLC1, which plays a vital role in the intron splicing of multiple group II introns, especially the rps16 intron, and is essential for early chloroplast development and seedling survival in rice.

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“…Cytokinins are the most significant phytohormones regulating the initiation and timing of leaf senescence (Xu and Huang, 2007; Reguera et al ., 2013; Jan et al ., 2019) and closely associated with the progress of leaf senescence in the stay-green mutant tasg1 of wheat (Wang et al ., 2016) and in the stay-green phenotype of maize ( Zea mays L.) (He et al ., 2005). The grain CTK concentrations were significantly and positively correlated with the grain-filling rate and maximum grain mass (Yang et al ., 2002; Yang et al ., 2008; Zhang et al ., 2009).…”

Section: Discussionmentioning

confidence: 99%

“…The MFs such as phosphoenolpyruvate carboxylase activity, phosphoenolpyruvate carboxykinase activity, sucrose-phosphate phosphatase activity were affected (Table S1). Of the most interesting, sucrose phosphate phosphatase was associated with source activity, enhancing transport of its resulting product sucrose from the photosynthetic tissues via the phloem into sink (Maloney et al ., 2015) and thus affecting progress of leaf senescence in wheat (Wang et al ., 2016). BP analysis showed that these processes were upregulated, indicating that in half-degrained flag leaf, nitrogen and carbon metabolism may be enhanced.…”

Section: Discussionmentioning

confidence: 99%

Source-Sink Modifications Affect Leaf Senescence and Grain Mass in Wheat

Zhang

et al. 2019

Preprint

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1 A field experiment was performed in wheat to investigate the responses of flag leaf and grain to 2 sink/source manipulations. The results showed that half-degraining delayed but defoliation (only 3 flag leaf left) enhanced the leaf senescence. Sink/source manipulations influenced the content of 4 1 1 Chloroplast Structure 1 2 At 16 DAM, visual observation did not find difference in senescing flag leaf between three 1 3 Fv/Fm 0.0 0.2 0.4 0.6 0.8

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OspTAC2 encodes a pentatricopeptide repeat protein and regulates rice chloroplast development

Cited by 37 publications

References 43 publications

WHITE STRIPE LEAF4 Encodes a Novel P-Type PPR Protein Required for Chloroplast Biogenesis during Early Leaf Development

WHITE STRIPE LEAF4 Encodes a Novel P-Type PPR Protein Required for Chloroplast Biogenesis during Early Leaf Development

OsSLC1 Encodes a Pentatricopeptide Repeat Protein Essential for Early Chloroplast Development and Seedling Survival

Source-Sink Modifications Affect Leaf Senescence and Grain Mass in Wheat

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