OsCOL16 , encoding a CONSTANS-like protein, represses flowering by up-regulating Ghd7 expression in rice

Wu, Weixun; Zheng, Xiaoming; Chen, Daibo; Zhang, Yingxin; Ma, Weiwei; Zhang, Huan; Sun, Lianping; Yang, Zhengfu; Zhao, Chunde; Zhan, Xiaodeng; Shen, Xihong; Yü, Ping; Fu, Yaping; Zhu, Shanshan; Cheng, Shifeng

doi:10.1016/j.plantsci.2017.04.004

Cited by 59 publications

(51 citation statements)

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“…The full‐length coding sequence of TIP3 without the stop codon was amplified and inserted into the transient expression vector pAN580 at the Bam HI site. The p35S: TIP3−GFP and p35S: OsMADS3−mCherry fusion constructs were transiently co‐transformed into the rice protoplast as described previously (Wu et al ., ). The fluorescence signal was detected with a laser scanning confocal microscope (Zeiss LSM700, Oberkochen, Germany).…”

Section: Methodsmentioning

confidence: 97%

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TDR INTERACTING PROTEIN 3, encoding a PHD‐finger transcription factor, regulates Ubisch bodies and pollen wall formation in rice

et al. 2019

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Summary Male reproductive development involves a complex series of biological events and precise transcriptional regulation is essential for this biological process in flowering plants. Several transcriptional factors have been reported to regulate tapetum and pollen development, however the transcriptional mechanism underlying Ubisch bodies and pollen wall formation remains less understood. Here, we characterized and isolated a male sterility mutant of TDR INTERACTING PROTEIN 3 (TIP3) in rice. The tip3 mutant displayed smaller and pale yellow anthers without mature pollen grains, abnormal Ubisch body morphology, no pollen wall formation, as well as delayed tapetum degeneration. Map‐based cloning demonstrated that TIP3 encodes a conserved PHD‐finger protein and further study confirmed that TIP3 functioned as a transcription factor with transcriptional activation activity. TIP3 is preferentially expressed in the tapetum and microspores during anther development. Moreover, TIP3 can physically interact with TDR, which is a key component of the transcriptional cascade in regulating tapetum development and pollen wall formation. Furthermore, disruption of TIP3 changed the expression of several genes involved in tapetum development and degradation, biosynthesis and transport of lipid monomers of sporopollenin in tip3 mutant. Taken together, our results revealed an unprecedented role for TIP3 in regulating Ubisch bodies and pollen exine formation, and presents a potential tool to manipulate male fertility for hybrid rice breeding.

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Section: Methodsmentioning

confidence: 97%

“…The pGBKT7 empty vector served as a negative control. Activity of β‐galactosidase was measured as reported previously (Wu et al ., ). The primers used for vector construction are listed in Table S2.…”

Section: Methodsmentioning

confidence: 97%

TDR INTERACTING PROTEIN 3, encoding a PHD‐finger transcription factor, regulates Ubisch bodies and pollen wall formation in rice

et al. 2019

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“…The COL genes belong to a larger transcription factor gene family named the B-BOX (BBX) family, which can be divided into five groups based on the presence of one or two B-BOX motifs and the presence or absence of the CCT domain [ 15 ]. Previous studies showed that most COL genes containing a CCT domain are involved in controlling the flowering time in some plant species [ 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification and characterization of CONSTANS-like gene family in radish (Raphanus sativus)

Wei

Wang

et al. 2018

PLoS ONE

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Floral induction that initiates bolting and flowering is crucial for reproductive fitness in radishes. CONSTANS-like (CO-like, COL) genes play an important role in the circadian clock, which ensures regular development through complicated time-keeping mechanisms. However, the specific biological and functional roles of each COL transcription factor gene in the radish remain unknown. In this study, we performed a genome-wide identification of COL genes in the radish genome of three cultivars including ‘Aokubi’, ‘kazusa’ and ‘WK10039’, and we analyzed their exon-intron structure, gene phylogeny and synteny, and expression levels in different tissues. The bioinformatics analysis identified 20 COL transcription factors in the radish genome, which were divided into three subgroups (Group I to Group III). RsaCOL-09 and RsaCOL-12 might be tandem duplicated genes, whereas the others may have resulted from segmental duplication. The Ka/Ks ratio indicated that all the COL genes in radish, Arabidopsis, Brassica rapa, Brassica oleracea, Capsella rubella and rice were under purifying selection. We identified 6 orthologous and 19 co-orthologous COL gene pairs between the radish and Arabidopsis, and we constructed an interaction network among these gene pairs. The expression values for each COL gene during vegetable and flower development showed that the majority of Group I members had similar expression patterns. In general, the expression of radish COL genes in Groups I and III decreased during development, whereas the expression of radish COL genes in Group II first increased and then decreased. Substantial numbers of radish COL genes were differentially expressed after vernalization treatment. The expression levels of RsaCOL-02 and RsaCOL-04 were significantly increased during vernalization treatment, while the expression of RsaCOL-10 was significantly decreased. These outcomes provide insights for improving the genetic control of bolting and flowering in radish and other root vegetable crops, and they facilitate genetic improvements to radish yields and quality.

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“…Unlike HD1, a set of B-box-containing proteins, including OsBBX5/OsCOL4, OsBBX7/OsCOL9, OsBBX10/OsCOL10, OsBBX14, OsBBX17/OsCOL16, OsBBX23/OsCOL13, OsBBX26/OsCOL15, and OsBBX27/ OsCO3, repress floral initiation in rice under long days and/or short days. These proteins predominantly upregulate the expression of the negative floral regulator GRAIN NUMBER, PLANT HEIGHT AND HEADING DATE 7 (GHD7) and inhibit the expression of the positive floral regulator RICE INDETERMINATE1 (RID1), thereby reducing the transcript abundance of the floral activators HD3a EARLY HEADING DATE 1 (EHD1) or RICE FLOWERING LOCUS T1 (RFT1) to suppress flowering (Kim et al 2008;Lee et al 2010;Bai et al 2016;Liu et al 2016;Sheng et al 2016;Tan et al 2016Tan et al , 2017Wu et al 2017Wu et al , 2018. CmBBX8 accelerates flowering by directly associating with the CmFT promoter region and upregulating its transcription in summer chrysanthemum (Wang et al 2019).…”

Section: Involvement Of Bbx In Floral Regulationmentioning

confidence: 99%

B‐box proteins: Pivotal players in light‐mediated development in plants

Song

Bian

Liu

et al. 2020

JIPB

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Light signals mediate a number of physiological and developmental processes in plants, such as flowering, photomorphogenesis, and pigment accumulation. Emerging evidence has revealed that a group of B‐box proteins (BBXs) function as central players in these light‐mediated developmental processes. B‐box proteins are a class of zinc‐coordinated transcription factors or regulators that not only directly mediate the transcription of target genes but also interact with various other factors to create a complex regulatory network involved in the precise control of plant growth and development. This review summarizes and highlights the recent findings concerning the critical regulatory functions of BBXs in photoperiodic flowering, light signal transduction and light‐induced pigment accumulation and their molecular modes of action at the transcriptional and post‐translational levels in plants

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OsCOL16 , encoding a CONSTANS-like protein, represses flowering by up-regulating Ghd7 expression in rice

Cited by 59 publications

References 50 publications

TDR INTERACTING PROTEIN 3, encoding a PHD‐finger transcription factor, regulates Ubisch bodies and pollen wall formation in rice

TDR INTERACTING PROTEIN 3, encoding a PHD‐finger transcription factor, regulates Ubisch bodies and pollen wall formation in rice

Genome-wide identification and characterization of CONSTANS-like gene family in radish (Raphanus sativus)

B‐box proteins: Pivotal players in light‐mediated development in plants

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