Orosphere assay: A method for propagation of head and neck cancer stem cells

Krishnamurthy, S; Nör, Jacques E.

doi:10.1002/hed.23076

Cited by 55 publications

(62 citation statements)

References 28 publications

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“…UM-SCC-1, UM-SCC-22A or UM-SCC-22B cells (1,000-3,000) were cultured in ultra-low attachment plates (Corning Inc., Corning, NY) with serum-free medium containing 0-50 ng/ml EGF, EC CM, or unconditioned medium for 7-15 days, as we showed (25). Orospheres, defined as non-adherent spheres of ≤25 cells, were photographed and counted under light microscopy.…”

Section: Methodsmentioning

confidence: 99%

Endothelial Cell-Secreted EGF Induces Epithelial to Mesenchymal Transition and Endows Head and Neck Cancer Cells with Stem-like Phenotype

et al. 2014

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Emerging evidence suggests that endothelial cell-secreted factors contribute to the pathobiology of squamous cell carcinoma (SCC) by enhancing invasive migration and resistance to anoikis. Here we report that SCC cells within the perivascular niche have undergone epithelial to mesenchymal transition (EMT) in a primary human SCC of a patient that developed distant metastases. Endothelial cell-secreted EGF induced EMT of human SCC cells in vitro and also induced acquisition of a stem-like phenotype. In vivo, tumor xenografts vascularized with EGF-silenced endothelial cells exhibited a smaller fraction of cancer stem-like cells (ALDH+CD44+) and were less invasive than tumors vascularized with control endothelial cells. Collectively, these results demonstrated that endothelial cell-EGF induces EMT and an acquisition of stem-like properties by head and neck tumor cells. On this basis, we suggest that vascular endothelial cells contribute to tumor dissemination by secreting factors that endow carcinoma cells with enhanced motility and stemness.

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Section: Methodsmentioning

confidence: 99%

Endothelial Cell-Secreted EGF Induces Epithelial to Mesenchymal Transition and Endows Head and Neck Cancer Cells with Stem-like Phenotype

et al. 2014

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“…strategy is suitable for the propagation of head and neck cancer cells and can retain the stemness and self-renewal capacity of these cells [71].…”

Section: Suspension Culturementioning

confidence: 99%

Strategies for Isolating and Enriching Cancer Stem Cells: Well Begun Is Half Done

Duan

Qiu

et al. 2013

Stem Cells and Development

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Cancer stem cells (CSCs) constitute a subpopulation of cancer cells that have the potential for self-renewal, multipotent differentiation, and tumorigenicity. Studies on CSC biology and CSC-targeted therapies depend on CSC isolation and/or enrichment methodologies. Scientists have conducted extensive research in this field since John Dick's group successfully isolated CSCs based on the expression of the CD34 and CD38 surface markers. Progress in CSC research has been greatly facilitated by the enrichment and isolation of these cells. In this review, we summarize the current strategies used in our and other laboratories for CSC isolation and enrichment, including methods based on stem cell surface markers, intracellular enzyme activity, the concentration of reactive oxygen species, the mitochondrial membrane potential, promoter-driven fluorescent protein expression, autofluorescence, suspension/adherent culture, cell division, the identification of side population cells, resistance to cytotoxic compounds or hypoxia, invasiveness/adhesion, immunoselection, and physical property. Although many challenges remain to be overcome, it is reasonable to believe that more reliable, efficient, and convenient methods will be developed in the near future.

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“…Additional studies at the University of Michigan have identified various CSC markers in HNSCC (e.g. ALDH, CD44, Bmi-1; [93–96]. Other markers also have been proposed (e.g.…”

Section: Clinical Relevance Of Cancer Stem Cells In Oral Tumorigenesismentioning

confidence: 99%

Oral epithelial stem cells—Implications in normal development and cancer metastasis

Papagerakis

Pannone

Zheng

et al. 2014

Experimental Cell Research

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Oral mucosa is continuously exposed to environmental forces and has to be constantly renewed. Accordingly, the oral mucosa epithelium contains a large reservoir of epithelial stem cells necessary for tissue homeostasis. Despite considerable scientific advances in stem cell behavior in a number of tissues, fewer studies have been devoted to the stem cells in the oral epithelium. Most of oral mucosa stem cells studies are focused on identifying cancer stem cells (CSC) in oral squamous cell carcinomas (OSCCs) among other head and neck cancers. OSCCs are the most prevalent epithelial tumors of the head and neck region, marked by their aggressiveness and invasiveness. Due to their highly tumorigenic properties, it has been suggested that CSC may be the critical population of cancer cells in the development of OSCC metastasis. This review presents a brief overview of epithelium stem cells with implications in oral health, and the clinical implications of the CSC concept in OSCC metastatic dissemination.

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Orosphere assay: A method for propagation of head and neck cancer stem cells

Cited by 55 publications

References 28 publications

Endothelial Cell-Secreted EGF Induces Epithelial to Mesenchymal Transition and Endows Head and Neck Cancer Cells with Stem-like Phenotype

Endothelial Cell-Secreted EGF Induces Epithelial to Mesenchymal Transition and Endows Head and Neck Cancer Cells with Stem-like Phenotype

Strategies for Isolating and Enriching Cancer Stem Cells: Well Begun Is Half Done

Oral epithelial stem cells—Implications in normal development and cancer metastasis

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