Origins of follicular cells and ontogeny of steroidogenesis in ovine fetal ovaries

Juengel, Jennifer L.; Sawyer, H. R.; Smith, Peter; Quirke, Laurel D.; Heath, D. A.; Lun, S.; Wakefield, St. John; McNatty, Kenneth P.

doi:10.1016/s0303-7207(02)00045-x

Cited by 95 publications

(87 citation statements)

References 19 publications

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“…The adverse effect of dominant interfering CREB protein expression on OSE cells reported here is also similar to that reported previously for granulosa cells (Somers et al 1999). This is consistent with the common histogenetic lineage of OSE and granulosa cells (Juengel et al 2002) and suggests that CREB-associated signalling may be important to maintain the OSE as well as follicular development.…”

Section: Discussionsupporting

confidence: 91%

cAMP response element-binding (CREB) signalling and ovarian surface epithelial cell survival

Gubbay¹,

Rae²,

McNeilly³

et al. 2006

Journal of Endocrinology

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cAMP response-element binding (CREB) transcription factors transduce cell survival responses to peptide hormones and growth factors in normal tissues and mutant CREB proteins are implicated in tumorigenesis. Ovarian cancer most frequently arises from the ovarian surface epithelium (OSE), possibly due to repeat inflammation-associated injury-repair episodes that promote neoplasia. We asked if post-receptor signalling involving the CREB family of proteins plays a role in OSE cell survival. In an ovine ovulation model, abundant expression of phospho-CREB/activating transcription factor (ATF) protein was detected immunohistochemically, strongly localised to OSE cells in the proximity of pre-ovulatory follicles. Treatment of primary sheep OSE cell cultures with LH stimulated cAMP accumulation and reduced apoptosis (caspase 3/7 activity) in response to serum withdrawal. When OSE cells were infected with an adenovirus containing a CRE-luciferase construct, exposure to LH and FSH induced CRE-directed transcription. Finally, when a non-phosphorylatable mutant of CREB (Ad CREB S133A ) was adenovirally expressed, apoptosis measured by activation of caspases was increased several fold relative to that caused by transfection with wild-type CREB (Ad CREB WT ) or lacZ (Ad lacZ). To test the potential clinical relevance of these findings, we expressed mutant CREB protein in normal human OSE cells from four women and a series of cell lines derived from human ovarian cancers. Infection with Ad CREB S133A markedly increased apoptosis in normal human OSE but had no detectable effect on apoptosis in any of the cancer cell lines. We conclude that CREB/ATF signalling is important for the maintenance of OSE cell survival in vitro and is altered in human cell lines derived from ovarian cancers.

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Section: Discussionsupporting

confidence: 91%

cAMP response element-binding (CREB) signalling and ovarian surface epithelial cell survival

Gubbay¹,

Rae²,

McNeilly³

et al. 2006

Journal of Endocrinology

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“…Previously, we have also demonstrated that the same BPA or DES postnatal treatment induces a high incidence of MOFs, suggesting that follicular assembly may be active during early postnatal life in lambs. This was surprising as most studies have suggested that a defined and finite pool of primordial follicles exists at birth in lambs (Juengel et al 2002, Padmanabhan et al 2007. However, based on a recent report that has proposed a new mechanism for generation of MOFs in the postnatal rat ovary, we cannot rule out the possibility that MOFs in xenoestrogentreated lambs are generated by fusion of adjacent growing follicles (Gaytán et al 2014).…”

Section: Discussionmentioning

confidence: 95%

Neonatal exposure to xenoestrogens impairs the ovarian response to gonadotropin treatment in lambs

Rivera¹,

Varayoud²,

Rodríguez³

et al. 2015

Reproduction

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Bisphenol A (BPA) and diethylstilbestrol (DES) are xenoestrogens, which have been associated with altered effects on reproduction. We hypothesized that neonatal xenoestrogen exposure affects the ovarian functionality in lambs. Thus, we evaluated the ovarian response to exogenous ovine FSH (oFSH) administered from postnatal day 30 (PND30) to PND32 in female lambs previously exposed to low doses of DES or BPA (BPA50: 50 mg/kg per day, BPA0.5: 0.5 mg/kg per day) from PND1 to PND14. We determined: i) follicular growth, ii) circulating levels of 17b-estradiol (E 2 ), iii) steroid receptors (estrogen receptor alpha, estrogen receptor beta, and androgen receptor (AR)) and atresia, and iv) mRNA expression levels of the ovarian bone morphogenetic protein (BMPs) system (BMP6, BMP15, BMPR1B, and GDF9) and FSH receptor (FSHR). Lambs neonatally exposed to DES or BPA showed an impaired ovarian response to oFSH with a lower number of follicles R2 mm in diameter together with a lower number of atretic follicles and no increase in E 2 serum levels in response to oFSH treatment. In addition, AR induction by oFSH was disrupted in granulosa and theca cells of lambs exposed to DES or BPA. An increase in GDF9 mRNA expression levels was observed in oFSH-primed lambs previously treated with DES or BPA50. In contrast, a decrease in BMPR1B was observed in BPA0.5-postnatally exposed lambs. The modifications in AR, GDF9, and BMPR1B may be associated with the altered ovarian function due to neonatal xenoestrogen exposure in response to an exogenous gonadotropin stimulus. These alterations may be the pathophysiological basis of subfertility syndrome in adulthood.Reproduction (2015) 149 645-655

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“…56: [162][163][164][165][166][167][168] 2010) he mammalian ovary is a heterogeneous organ consisting of follicles and corpora lutea at various developmental stages. In the fetal ovaries, germ cells remain in clusters, which here will be referred to as egg nests [1][2][3]. Cells of a nest die, causing the nest to break into smaller nests.…”

mentioning

confidence: 99%

Formation of Primordial Follicles and Immunolocalization of PTEN, PKB and FOXO3A Proteins in the Ovaries of Fetal and Neonatal Pigs

et al. 2010

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Abstract. The assembly of primordial follicles and subsequent development and transition of the primordial follicle to the primary follicle are critical processes in ovarian biology. In order to examine follicle formation and development in fetal and neonatal pigs, ovarian samples were obtained from a famous local breed of Chinese pigs, Erhualian pigs, ranging in age from 50 days postcoitum to 1 day postpartum in our current study. Morphological changes in the ovaries of the fetal and neonatal pigs indicated that egg nests were the earliest recognizable gamete cells. The proportion of egg nests decreased from 98.4 to 25.6% and the proportion of single follicles increased from 1.6 to 74.4% between 70 and 90 days postcoitum. The proportions of primordial follicles increased between 70 and 90 days postcoitum but decreased from 90 days postcoitum to 1 day postpartum. Our results suggested that the key stage of primordial follicle formation was between 70 and 90 days postcoitum and that the major stage of transition from primordial follicles into primary follicles was between 90 days postcoitum and 1 day postpartum. Experiments were also conducted to examine the localization of PTEN, PKB and FOXO3A proteins in the porcine ovaries by immunohistochemistry and immunoblotting. The results indicated that PTEN, PKB and FOXO3A were localized in the germ cells of egg nests, cytoplasm of oocytes and granulosa cells of follicles ranging from the primordial to secondary stages and that the staining intensity was weak in granulosa cells but strong in oocytes. The different staining patterns of PTEN, FOXO3A and PKB suggested that these proteins were expressed in a stage-and cell-specific manner during ovarian follicle formation and development in the fetal and neonatal pig. Key words: FOXO3A, Ovary, Pig, Primordial follicle, PTEN (J. Reprod. Dev. 56: [162][163][164][165][166][167][168] 2010) he mammalian ovary is a heterogeneous organ consisting of follicles and corpora lutea at various developmental stages. In the fetal ovaries, germ cells remain in clusters, which here will be referred to as egg nests [1][2][3]. Cells of a nest die, causing the nest to break into smaller nests. This process is repeated until a few individual eggs remain. At the time of birth, the mouse ovary is populated predominantly by primordial follicles, each composed of a meiotically-arrested primordial oocyte enclosed within several flattened pregranulosa cells [4][5][6]. Although many reports have indicated that primordial follicles mainly form in fetal porcine ovaries [7][8][9], the precise stage of egg nest breakdown to form primordial follicles is not clear.The oocytes in primordial follicles are thought to represent the entire pool of gametes available to a female throughout her reproductive life, although it has been suggested that there are postnatal germ line stem cells in the adult ovary [10]. Primordial follicle activation is irreversible, and is the metered process by which primordial follicles are removed from the long-lived reserve pool t...

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Origins of follicular cells and ontogeny of steroidogenesis in ovine fetal ovaries

Cited by 95 publications

References 19 publications

cAMP response element-binding (CREB) signalling and ovarian surface epithelial cell survival

cAMP response element-binding (CREB) signalling and ovarian surface epithelial cell survival

Neonatal exposure to xenoestrogens impairs the ovarian response to gonadotropin treatment in lambs

Formation of Primordial Follicles and Immunolocalization of PTEN, PKB and FOXO3A Proteins in the Ovaries of Fetal and Neonatal Pigs

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