Original article Effect of oral administration of pig spinal cord hydrolysate on clinical and histopathological symptoms of experimental allergic encephalomyelitis in rats

Kasarełło, Kaja; Gadamski, R; Piotrowski, Piotr; Kurzepa, Katarzyna; Kwiatkowska-Patzer, B; Lipkowski, Andrzej W.

doi:10.5114/fn.2015.52409

Cited by 4 publications

(4 citation statements)

References 39 publications

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“…Administration of specific encephalitogenic antigens by various routes, including mucosal, i.v., i.p., s.c. and oral, can induce immune tolerance in EAE [7, 23, 24]. DCs, as professional APCs, can be induced into a tolerogenic phenotype, with the ability to promote the function of regulatory T cells or inhibit activation of autoreactive T cells [25–27].…”

Section: Discussionmentioning

confidence: 99%

Selective depletion of CD11c⁺CD11b⁺ dendritic cells partially abrogates tolerogenic effects of intravenous MOG in murine EAE

Wang

Ćirić

et al. 2016

Eur J Immunol

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Intravenous (i.v.) injection of a soluble myelin antigen can induce tolerance, which effectively ameliorates experimental autoimmune encephalomyelitis (EAE). We have previously shown that i.v. MOG induces tolerance in EAE and expands a subpopulation of tolerogenic CD11c+CD11b+ dendritic cells (DCs) with an immature phenotype having low expression of IA and co-stimulatory molecules CD40, CD86, and CD80. Here we further investigate the role of tolerogenic DCs in i.v. tolerance by injecting clodronate-loaded liposomes, which selectively deplete CD11c+CD11b+ and immature DCs, but not CD11c+CD8+ DCs and mature DCs. I.v. MOG-induced suppression of EAE was partially, yet significantly, blocked by CD11c+CD11b+ DC depletion. While i.v. MOG inhibited IA, CD40, CD80, CD86 expression and induced TGF-β, IL-27, IL-10 production in CD11c+CD11b+ DCs, these effects were abrogated after injection of clodronate-loaded liposomes. Depletion of CD11c+CD11b+ DCs also precluded i.v. autoantigen-induced T-cell tolerance, such as decreased production of IL-2, IFN-γ, IL-17 and numbers of IL-2+, IFN-γ+, and IL-17+ CD4+ T cells, as well as an increased proportion of CD4+CD25+Foxp3+ regulatory T cells and CD4+IL-10+Foxp3− Tr1 cells. CD11c+CD11b+ DCs, through low expression of IA and co-stimulatory molecules as well as high expression of TGF-β, IL-27 and IL-10, play an important role in i.v. tolerance-induced EAE suppression.

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Section: Discussionmentioning

confidence: 99%

Selective depletion of CD11c⁺CD11b⁺ dendritic cells partially abrogates tolerogenic effects of intravenous MOG in murine EAE

Wang

Ćirić

et al. 2016

Eur J Immunol

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“…Male Lewis rats were inoculated subcutaneously (sc) in the pad of the left hind paw with 100 μ L antigenic emulsion containing equal volumes of saline with 20 μ g of guinea pig myelin basic protein (MBP) (Sigma-Aldrich, St. Louis, MO) and complete Freund's adjuvant (CFA) (Chondrex, Redmond, WA, USA) with Mycobacterium tuberculosis (2 mg/mL). Daily weight was recorded, and clinical signs were evaluated, using the following 5-grade scale [ 23 ]: 0, no clinical signs; 1, limp tail; 2, hind leg weakness; 3, paraplegia and incontinence; 4, quadriplegia; and 5, moribundity or death.…”

Section: Methodsmentioning

confidence: 99%

Therapeutic Potential of Pien Tze Huang on Experimental Autoimmune Encephalomyelitis Rat

Qiu

Hui

et al. 2018

Journal of Immunology Research

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Multiple sclerosis (MS) is a chronic inflammatory demyelinating disease of the central nervous system (CNS). There is still lack of commercially viable treatment currently. Pien Tze Huang (PZH), a traditional Chinese medicine, has been proved to have anti-inflammatory, neuroprotective, and immunoregulatory effects. This study investigated the possible therapeutic effects of PZH on experimental autoimmune encephalomyelitis (EAE) rats, a classic animal model of MS. Male Lewis rats were immunized with myelin basic protein (MBP) peptide to establish an EAE model and then treated with three doses of PZH. Clinical symptoms, organ coefficient, histopathological features, levels of proinflammatory cytokines, and chemokines as well as MBP and Olig2 were analyzed. The results indicated that PZH ameliorated the clinical severity of EAE rats. It also remarkably reduced inflammatory cell infiltration in the CNS of EAE rats. Furthermore, the levels of IL-17A, IL-23, CCL3, and CCL5 in serum and the CNS were significantly decreased; the p-P65 and p-STAT3 levels were also downregulated in the CNS, while MBP and Olig2 in the CNS of EAE rats had a distinct improvement after PZH treatment. In addition, PZH has no obvious toxicity at the concentration of 0.486 g/kg/d. This study demonstrated that PZH could be used to treat MS.

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“…These data suggest that up-regulation of HO-1 may contribute to diminished neuroprotective benefits of EGCG alone in this EAE model [11]. It has been shown that usage of preparations containing pig spinal cord hydrolysate, as a source of broad spectrum myelin antigens, is able to diminish clinical symptoms of ongoing EAE [13].…”

Section: Real-time Pcrmentioning

confidence: 91%

Effects of green tea epigallocatechin-3-gallate on the proteolipid protein and oligodendrocyte transcription factor 1 messenger RNA gene expression in a mouse model of multiple sclerosis

Semnani¹,

Mashayekhi²,

Azarnia³

et al. 2017

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A b s t r a c t The cuprizone multiple sclerosis (MS) animal model is characteristic for toxic demyelination and represents a reversible demyelination and remyelination system. It has been shown that green tea epigallocatechin-3-gallate (EGCG) might be effective in improving the symptoms and pathological conditions associated with autoimmune inflammatory diseases in several animal models. In this study the effects of EGCG on proteolipid protein (PLP) and oligodendrocyte transcription factor 1 (Olig1) expression in the cerebral cortex of a murine model of cuprizone-induced demyelination was investigated. C57BL/6 mice were treated with cuprizone for six weeks in order to induce demyelination. Immediately after the cessation of cuprizone the animals were divided into 6 groups (n = 10 for each group). The first two groups were injected intraperitoneally (IP) with EGCG in the amount of 50 mg/kg/daily body weight for 2 and 4 weeks. The second two groups (SHAM) were injected IP with phosphate-buffered saline (PBS) for

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Original article Effect of oral administration of pig spinal cord hydrolysate on clinical and histopathological symptoms of experimental allergic encephalomyelitis in rats

Abstract: A b s t r a c t Oral tolerance is the natural occurring phenomenon of a decreased immune response to previously fed antigens, which prevents induction of a response to dietary antigens. One of the mechanisms is deletion of T lym-

Cited by 4 publications

References 39 publications

Selective depletion of CD11c⁺CD11b⁺ dendritic cells partially abrogates tolerogenic effects of intravenous MOG in murine EAE

Selective depletion of CD11c⁺CD11b⁺ dendritic cells partially abrogates tolerogenic effects of intravenous MOG in murine EAE

Therapeutic Potential of Pien Tze Huang on Experimental Autoimmune Encephalomyelitis Rat

Effects of green tea epigallocatechin-3-gallate on the proteolipid protein and oligodendrocyte transcription factor 1 messenger RNA gene expression in a mouse model of multiple sclerosis

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Original article Effect of oral administration of pig spinal cord hydrolysate on clinical and histopathological symptoms of experimental allergic encephalomyelitis in rats

Abstract: A b s t r a c t Oral tolerance is the natural occurring phenomenon of a decreased immune response to previously fed antigens, which prevents induction of a response to dietary antigens. One of the mechanisms is deletion of T lym-

Cited by 4 publications

References 39 publications

Selective depletion of CD11c+CD11b+ dendritic cells partially abrogates tolerogenic effects of intravenous MOG in murine EAE

Selective depletion of CD11c+CD11b+ dendritic cells partially abrogates tolerogenic effects of intravenous MOG in murine EAE

Therapeutic Potential of Pien Tze Huang on Experimental Autoimmune Encephalomyelitis Rat

Effects of green tea epigallocatechin-3-gallate on the proteolipid protein and oligodendrocyte transcription factor 1 messenger RNA gene expression in a mouse model of multiple sclerosis

Contact Info

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Resources

About

Selective depletion of CD11c⁺CD11b⁺ dendritic cells partially abrogates tolerogenic effects of intravenous MOG in murine EAE

Selective depletion of CD11c⁺CD11b⁺ dendritic cells partially abrogates tolerogenic effects of intravenous MOG in murine EAE