2022
DOI: 10.1002/smsc.202270012
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Abstract: Oridonin Inhibits SARS‐CoV‐2 Oridonin, a natural product extracted from Rabdosia rubescens , possesses a wide range of pharmacological properties, including anti‐inflammatory, anti‐cancer, anti‐microbial, neuroprotection, immunoregulation, etc. In article number 2100124 , Baisen Zhong, Litao Sun, and co‐workers demonstrate that Oridonin targets the SARS‐CoV‐2 3CL protease by covalently binding to cysteine145 in its active pocket to exert an anti‐SARS‐CoV… Show more

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Cited by 12 publications
(9 citation statements)
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“…Oridonin has been identified as an inhibitor of M pro with an IC 50 value of 4.67 µM, which confirmed a previous study showing its M pro inhibitory activity (IC 50 2.16 µM [ 32 ]). From the literature, it is known that oridonin covalently modifies M pro by targeting Cys145.…”
Section: Resultssupporting
confidence: 86%
“…Oridonin has been identified as an inhibitor of M pro with an IC 50 value of 4.67 µM, which confirmed a previous study showing its M pro inhibitory activity (IC 50 2.16 µM [ 32 ]). From the literature, it is known that oridonin covalently modifies M pro by targeting Cys145.…”
Section: Resultssupporting
confidence: 86%
“…Oridonin has been identified as an inhibitor of M pro with an IC50 value of 4.67 µM, which confirmed the previous study with its M pro inhibitory activity (IC50 2.16 µM [32]), and we learned from the literature that Oridonin covalently modifies the M pro targeting Cys145. M pro crystal structure in complex with oridonin (PDB ID: 7VIC [32],…”
Section: >10supporting
confidence: 88%
“…As noted above, we purified the tcOTU domain and used it as the bait to perform a fluorescence‐based thermal shift assay (TSA). In this assay, using a sensitive fluorescent dye to monitor protein thermal unfolding, the ligand‐binding affinity can be assessed from the shift of the unfolding temperature (Δ T m ) obtained in the presence of ligands relative to that obtained in the absence of ligands 16 . Based on this, 5090 compounds including 2356 Food and Drug Administration (FDA)‐approved drugs and 2734 natural bioactive products were screened by this reporting assay (Supporting Information: Table ).…”
Section: Resultsmentioning
confidence: 99%