Organoids of the Female Reproductive Tract: Innovative Tools to Study Desired to Unwelcome Processes

Heremans, Ruben; Jan, Žiga; Timmerman, Dirk; Vankelecom, Hugo

doi:10.3389/fcell.2021.661472

Cited by 23 publications

(27 citation statements)

References 165 publications

(224 reference statements)

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“…Numerous studies have featured the generation of organoids derived from primary tissues or hPSCs, such as the ovaries, fallopian tubes, endometrium, and cervix. Organoids can also be generated from disorders of the FRS such as endometriosis and cancer [ 86 ].…”

Section: Bmps Signaling In Female Reproductive Systemmentioning

confidence: 99%

The Role of BMP Signaling in Female Reproductive System Development and Function

Magro-Lopez

Muñoz‐Fernández

2021

IJMS

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Bone morphogenetic proteins (BMPs) are a group of multifunctional growth factors that belong to the transforming growth factor-β (TGF-β) superfamily of proteins. Originally identified by their ability to induce bone formation, they are now known as essential signaling molecules that regulate the development and function of the female reproductive system (FRS). Several BMPs play key roles in aspects of reproductive system development. BMPs have also been described to be involved in the differentiation of human pluripotent stem cells (hPSCs) into reproductive system tissues or organoids. The role of BMPs in the reproductive system is still poorly understood and the use of FRS tissue or organoids generated from hPSCs would provide a powerful tool for the study of FRS development and the generation of new therapeutic perspectives for the treatment of FRS diseases. Therefore, the aim of this review is to summarize the current knowledge about BMP signaling in FRS development and function.

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Section: Bmps Signaling In Female Reproductive Systemmentioning

confidence: 99%

The Role of BMP Signaling in Female Reproductive System Development and Function

Magro-Lopez

Muñoz‐Fernández

2021

IJMS

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“…Studies in cultured stromal cells are one key model, as they are amenable to mechanistic studies; however, in vitro models in which to study epithelial cells have proven challenging. Recently, however, innovative organoid culture systems have been developed that provide a platform to study processes intrinsic to endometrial epithelial cells [ 23 , 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

Progesterone Signaling in Endometrial Epithelial Organoids

Hewitt

Wang

et al. 2022

Cells

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For pregnancy to be established, uterine cells respond to the ovarian hormones, estrogen, and progesterone, via their nuclear receptors, the estrogen receptor (ESR1) and progesterone receptor (PGR). ESR1 and PGR regulate genes by binding chromatin at genes and at distal enhancer regions, which interact via dynamic 3-dimensional chromatin structures. Endometrial epithelial cells are the initial site of embryo attachment and invasion, and thus understanding the processes that yield their receptive state is important. Here, we cultured and treated organoids derived from human epithelial cells, isolated from endometrial biopsies, with estrogen and progesterone and evaluated their transcriptional profiles, their PGR cistrome, and their chromatin conformation. Progesterone attenuated estrogen-dependent gene responses but otherwise minimally impacted the organoid transcriptome. PGR ChIPseq peaks were co-localized with previously described organoid ESR1 peaks, and most PGR and ESR1 peaks were in B (inactive) compartment regions of chromatin. Significantly more ESR1 peaks were assigned to estrogen-regulated genes by considering chromatin loops identified using HiC than were identified using ESR1 peak location relative to closest genes. Overall, the organoids model allowed a definition of the chromatin regulatory components governing hormone responsiveness.

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“…Among these studies, it can be mentioned to the successful 3D cervical organoids from ecto-and endocervical epithelial cells (Maru et al, 2019(Maru et al, , 2020. Although studies in this area are very limited, but promising results have been reported by researchers (Heremans et al, 2021).…”

Section: Cervical Organoidmentioning

confidence: 99%

Stem cells technology as a platform for generating reproductive system organoids and treatment of infertility‐related diseases

Jahanbani

Shafiee

Davaran

et al. 2021

Cell Biology International

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In recent years, stem cells have known as a helpful biological tool for the accurate diagnosis, treatment and recognition of diseases. Using stem cells as biomarkers have presented high potential in the early detection of many diseases. Another advancement in stem cell technology includes stem cell derived organoids model that could be a promising platform for diagnosis and modeling different diseases.Furthermore, therapeutic capabilities of stem cell therapy have increased hope in the face of different disability managements. All of these technologies are also widely used in reproductive related diseases especially in today's world that many couples encounter infertility problems. However, with the aid of numerous improvements in the treatment of infertility, over 80% of couples who dreamed of having children could now have children. Due to the fact that infertility has many negative effects on personal and social lives of young couples, many researchers have focused on the treatment of male and female reproductive system abnormalities with different types of stem cells, including embryonic stem cells, bone marrow mesenchymal stem cells (MSCs), and umbilical cord-derived MSCs. Also, design and formation of reproductive system organoids provide a fascinating window into disease modeling, drug screening, personalized therapy, and regeneration medicine. Utilizing these techniques to study, model and treat the infertility-related diseases has drawn attention of many scientists. This review explains different applications of stem cells in generating reproductive system organoids and stem cell-based therapies for male and female infertility related diseases treatment.

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Organoids of the Female Reproductive Tract: Innovative Tools to Study Desired to Unwelcome Processes

Cited by 23 publications

References 165 publications

The Role of BMP Signaling in Female Reproductive System Development and Function

The Role of BMP Signaling in Female Reproductive System Development and Function

Progesterone Signaling in Endometrial Epithelial Organoids

Stem cells technology as a platform for generating reproductive system organoids and treatment of infertility‐related diseases

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