Organoids can be established reliably from cryopreserved biopsy catheter-derived endometrial tissue of infertile women

Bui, Bich Ngoc; Boretto, Matteo; Kobayashi, Hiroto; Hoesel, Marliek van; Steba, G; Hoogenhuijze, Nienke van; Broekmans, Frank J.; Vankelecom, Hugo; Torrance, Helen L.

doi:10.1016/j.rbmo.2020.03.019

Cited by 26 publications

(17 citation statements)

References 10 publications

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“…By exposure to a specific hormone treatment protocol encompassing E2 and progesterone (P4), organoids mimicked the full menstrual cycle as well as incipient decidualization (Table 1 and Supplementary Table 3). The robustness of the endometrial organoid platform has been validated by IHC, electron microscopy, array Comparative Genomic Hybridization (aCGH) and transcriptome analysis and, in addition, ion channel functionality and ciliogenesis was demonstrated using these organoid systems (Fitzgerald et al, 2019;Haider et al, 2019;Hennes et al, 2019;Cochrane et al, 2020;Bui et al, 2020;Luddi et al, 2020;Syed et al, 2020). Two groups achieved to derive organoids from trophoblasts, offering the appealing possibility to study trophoblast-endometrium crosstalk in vitro (Haider et al, 2018;Turco et al, 2018).…”

Section: Endometrium Healthy Endometriummentioning

confidence: 99%

Organoids of the Female Reproductive Tract: Innovative Tools to Study Desired to Unwelcome Processes

Heremans

Jan

Timmerman

et al. 2021

Front. Cell Dev. Biol.

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The pelviperineal organs of the female reproductive tract form an essential cornerstone of human procreation. The system comprises the ectodermal external genitalia, the Müllerian upper-vaginal, cervical, endometrial and oviductal derivatives, and the endodermal ovaries. Each of these organs presents with a unique course of biological development as well as of malignant degeneration. For many decades, various preclinical in vitro models have been employed to study female reproductive organ (patho-)biology, however, facing important shortcomings of limited expandability, loss of representativeness and inadequate translatability to the clinic. The recent emergence of 3D organoid models has propelled the field forward by generating powerful research tools that in vitro replicate healthy as well as diseased human tissues and are amenable to state-of-the-art experimental interventions. Here, we in detail review organoid modeling of the different female reproductive organs from healthy and tumorigenic backgrounds, and project perspectives for both scientists and clinicians.

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Section: Endometrium Healthy Endometriummentioning

confidence: 99%

Organoids of the Female Reproductive Tract: Innovative Tools to Study Desired to Unwelcome Processes

Heremans

Jan

Timmerman

et al. 2021

Front. Cell Dev. Biol.

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“…This has been demonstrated using tissue from pancreas, colon, thyroid, lung, renal and liver tumours, where tissue was frozen in cryopreservation solution and showed that organoid establishment was possible from the thawed tissue [32,111]. It has also been demonstrated that organoids established from both fresh and frozen endometrial tissue had comparable organoid formation, morphology, expression of markers, proliferation rates and response to treatment [112]. This would increase the accessibility of tissue for organoid culture and has the potential to allow many more researchers to participate in studies using these tissues.…”

Section: Cryopreservationmentioning

confidence: 92%

Patient-Derived Organoids as a Model for Cancer Drug Discovery

Rae

Amato

Braconi

2021

IJMS

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In the search for the ideal model of tumours, the use of three-dimensional in vitro models is advancing rapidly. These are intended to mimic the in vivo properties of the tumours which affect cancer development, progression and drug sensitivity, and take into account cell–cell interactions, adhesion and invasiveness. Importantly, it is hoped that successful recapitulation of the structure and function of the tissue will predict patient response, permitting the development of personalized therapy in a timely manner applicable to the clinic. Furthermore, the use of co-culture systems will allow the role of the tumour microenvironment and tissue–tissue interactions to be taken into account and should lead to more accurate predictions of tumour development and responses to drugs. In this review, the relative merits and limitations of patient-derived organoids will be discussed compared to other in vitro and ex vivo cancer models. We will focus on their use as models for drug testing and personalized therapy and how these may be improved. Developments in technology will also be considered, including the use of microfluidics, 3D bioprinting, cryopreservation and circulating tumour cell-derived organoids. These have the potential to enhance the consistency, accessibility and availability of these models.

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“…Abbreviation: EMSC, endometrial stromal cells; hESCFCs, human embryonic stem cell-derived feeder cells; SEM, standard error of the mean utilized thawed endometrial cells that were cultured for more than 3 months. Indeed, cryopreservation of freshly biopsied tissue was challenged [41]. These findings are critical for clinical application from the viewpoint that reproductive endocrinologists prepare patient-derived endometrial cells for ongoing fertility treatment in a clinical setting.…”

Section: Potential Clinical Applicationmentioning

confidence: 99%

Endometrial regeneration with endometrial epithelium: homologous orchestration with endometrial stroma as a feeder

et al. 2021

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Background Thin endometrium adversely affects reproductive success rates with fertility treatment. Autologous transplantation of exogenously prepared endometrium can be a promising therapeutic option for thin endometrium; however, endometrial epithelial cells have limited expansion potential, which needs to be overcome in order to make regenerative medicine a therapeutic strategy for refractory thin endometrium. Here, we aimed to perform long-term culture of endometrial epithelial cells in vitro. Methods We prepared primary human endometrial epithelial cells and endometrial stromal cells and investigated whether endometrial stromal cells and human embryonic stem cell-derived feeder cells could support proliferation of endometrial epithelial cells. We also investigated whether three-dimensional culture can be achieved using thawed endometrial epithelial cells and endometrial stromal cells. Results Co-cultivation with the feeder cells dramatically increased the proliferation rate of the endometrial epithelial cells. We serially passaged the endometrial epithelial cells on mouse embryonic fibroblasts up to passage 6 for 4 months. Among the human-derived feeder cells, endometrial stromal cells exhibited the best feeder activity for proliferation of the endometrial epithelial cells. We continued to propagate the endometrial epithelial cells on endometrial stromal cells up to passage 5 for 81 days. Furthermore, endometrial epithelium and stroma, after the freeze-thaw procedure and sequential culture, were able to establish an endometrial three-dimensional model. Conclusions We herein established a model of in vitro cultured endometrium as a potential therapeutic option for refractory thin endometrium. The three-dimensional culture model with endometrial epithelial and stromal cell orchestration via cytokines, membrane-bound molecules, extracellular matrices, and gap junction will provide a new framework for exploring the mechanisms underlying the phenomenon of implantation. Additionally, modified embryo culture, so-called “in vitro implantation”, will be possible therapeutic approaches in fertility treatment.

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Organoids can be established reliably from cryopreserved biopsy catheter-derived endometrial tissue of infertile women

Cited by 26 publications

References 10 publications

Organoids of the Female Reproductive Tract: Innovative Tools to Study Desired to Unwelcome Processes

Organoids of the Female Reproductive Tract: Innovative Tools to Study Desired to Unwelcome Processes

Patient-Derived Organoids as a Model for Cancer Drug Discovery

Endometrial regeneration with endometrial epithelium: homologous orchestration with endometrial stroma as a feeder

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