Organization of the ribosomal RNA gene cluster in Lytechinus variegatus. Restriction analysis and cloning of restriction fragments.

Blin, Nikolaus; Sperrazza, Joan M.; Wilson, Frances E.; Bieber, David; Mickel, F S; Stafford, Darrel W.

doi:10.1016/s0021-9258(17)30131-x

Cited by 25 publications

(4 citation statements)

References 35 publications

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“…Another specific polymerase II product, histone mRNA, was also synthesized by RNA polymerase II (Levy et al, 1978; see below) in these nuclei. RNA polymerase I activity was detected by resistance to high concentrations of a-amanitin (Figure 1) and hybridization to the cloned sea urchin rDNA plasmids pLv4 and pLvl334 (Blin et al, 1979). These two plasmids contained the entire sea urchin ribosomal RNA repeat.…”

Section: Resultsmentioning

confidence: 99%

“…[a-32P]GTP (20-50 Ci/mmol) was synthesized by the method of Reeve & Huang (1979) or purchased from ICN. Plasmids pLv4, pLvl334 (Blin et al, 1979), and pLu305 (Lu et al, 1981) were gifts of Dr. Darrel Stafford. Plasmids pSpl02 and pSpl 17 (Levy et al, 1978) were gifts of Dr. Larry Kedes. Preparation of Nuclei.…”

Section: Methodsmentioning

confidence: 99%

“…0006-2960/83/0422-0645S01.50/0 © 1983 American Chemical Society The onset of synthesis of the small structural RNAs, 5S and tRNAs, by RNA polymerase III occurs early in sea urchin development (O'Melia, 1979a,b;Nijhawan & Marzluff, 1979). Since the 18S and 26S rRNAs are transcribed as a large precursor (Blin et al, 1979;Bieber & Stafford, 1979;Griffith & Humphreys, 1979), the period of development when their synthesis is initiated has been more difficult to identify.…”

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confidence: 99%

“…The bands are labeled corresponding to the map in Figure3B (D, E, and F). (B) A schematic diagram of the recombinant ribosomal RNA plasmid is shown [fromBlin et al (1979)]. Plasmid DNA is denoted as a solid horizontal line, the L. varigatus DNA as open boxes, the regions corresponding to the mature RNA species as closed boxes, and the transcribed spacer region as hatched boxes.…”

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A factor in sea urchin eggs inhibits transcription in isolated nuclei by sea urchin RNA polymerase III

Morris¹,

Marzluff²

1983

Biochemistry

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Isolated nuclei from sea urchin embryos synthesize RNA at a rate comparable to other animal cell nuclei. All three RNA polymerases are active as judged by alpha-amanitin sensitivity and hybridization to specific cloned DNAs. Extracts were prepared from sea urchin eggs and embryos by extraction with 0.35 M KCl. None of the crude extracts had a large effect on total RNA synthesis. However, extracts from sea urchin eggs inhibited RNA polymerase III activity in nuclei from blastula and gastrula embryos. There was no effect on the synthesis of ribosomal RNA by RNA polymerase I or on the synthesis of two RNA polymerase II products, histone mRNA and the sea urchin analogue of U1 RNA. The inhibitor is present in two different species of sea urchin and has been 50-fold purified by diethylaminoethylcellulose and hydroxylapatite chromatography. The inhibitor is not present in extracts prepared from sea urchin blastula embryos.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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A factor in sea urchin eggs inhibits transcription in isolated nuclei by sea urchin RNA polymerase III

Morris¹,

Marzluff²

1983

Biochemistry

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Cloning and Structural Analysis of Genes Encoding Prostatein, the Major Androgen Dependent Secretory Protein of Rat Ventral Prostate

Viskochil¹,

Perry²,

Lea³

et al. 1984

Regulation of Target Cell Responsiveness

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Characterization of cloned rat ribosomal DNA fragments

1981

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Two Charon 4A lambda bacteriophage clones were characterized which contain all and part o the 18S ribosomal DNA of the rat. One clone contained two Eco RI fragments which include the whole 18S ribosomal RNA region and part of 28S ribosomal RNA region. The other clone contained an Eco RI fragment which covers part of 18S ribosomal RNA region. There were differences between the two clones in the non-transcribed spacer regions suggesting that there is heterogeneity in the non-transcribed spacer regions of rat ribosomal genes. The restriction maps of the two clones were compared to the restriction map of the cloned mouse ribosomal DNA. Eco RI, Hind III, Pst I, and Bam HI sites in 18S ribosomal RNA regions were in the same places in mouse and rat DNA but the restriction sites in the 5'-spacer regions were different.

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Organization of the ribosomal RNA gene cluster in Lytechinus variegatus. Restriction analysis and cloning of restriction fragments.

Cited by 25 publications

References 35 publications

A factor in sea urchin eggs inhibits transcription in isolated nuclei by sea urchin RNA polymerase III

A factor in sea urchin eggs inhibits transcription in isolated nuclei by sea urchin RNA polymerase III

Cloning and Structural Analysis of Genes Encoding Prostatein, the Major Androgen Dependent Secretory Protein of Rat Ventral Prostate

Characterization of cloned rat ribosomal DNA fragments

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