Organization of the pronephric kidney revealed by large-scale gene expression mapping

Raciti, Daniela; Reggiani, Luca; Geffers, Lars; Jiang, Qiuhong; Bacchion, Francesca; Subrizi, Astrid; Clements, D. L.; Tindal, Christopher; Davidson, Duncan; Kaissling, Brigitte; Brändli, André W.

doi:10.1186/gb-2008-9-5-r84

Cited by 99 publications

(124 citation statements)

References 93 publications

(68 reference statements)

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“…As a matter of fact, a significant conservation in nephron organization between the kidneys of Xenopus embryos and adult mice was demonstrated in a large-scale comparative gene expression study. 64 Our data clearly show that both QDs and gH625-QDs do not affect the survival of embryos. The embryonic phenotype, observed in the stereomicroscope, appears unaltered for both gH625-QDs and QDs.…”

Section: Discussionsupporting

confidence: 52%

Daphnia magna and Xenopus laevis as in vivo models to probe toxicity and uptake of quantum dots functionalized with gH625

Galdiero¹,

Falanga²,

Siciliano³

et al. 2017

IJN

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The use of quantum dots (QDs) for nanomedicine is hampered by their potential toxicologic effects and difficulties with delivery into the cell interior. We accomplished an in vivo study exploiting Daphnia magna and Xenopus laevis to evaluate both toxicity and uptake of QDs coated with the membranotropic peptide gH625 derived from the glycoprotein H of herpes simplex virus and widely used for drug delivery studies. We evaluated and compared the effects of QDs and gH625-QDs on the survival, uptake, induction of several responsive pathways and genotoxicity in D. magna , and we found that QDs coating plays a key role. Moreover, studies on X. laevis embryos allowed to better understand their cell/tissue localization and delivery efficacy. X. laevis embryos raised in Frog Embryo Teratogenesis Assay- Xenopus containing QDs or gH625-QDs showed that both nanoparticles localized in the gills, lung and intestine, but they showed different distributions, indicating that the uptake of gH625-QDs was enhanced; the functionalized QDs had a significantly lower toxic effect on embryos’ survival and phenotypes. We observed that D. magna and X. laevis are useful in vivo models for toxicity and drug delivery studies.

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Section: Discussionsupporting

confidence: 52%

Daphnia magna and Xenopus laevis as in vivo models to probe toxicity and uptake of quantum dots functionalized with gH625

Galdiero¹,

Falanga²,

Siciliano³

et al. 2017

IJN

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“…We targeted this question by studying to what extent a panel of nephron segment-specific markers 24 would become induced ex vivo.…”

Section: Tubule Induction Of the Kidney Mesenchyme Progenitor Cells Lmentioning

confidence: 99%

“…[16][17][18][19][20] However, it is currently still impossible to target the cellular and molecular genetic details before or during the transmission and transduction of the inductive signals. [21][22][23][24] We show here that the dissociated and reaggregated kidney mesenchyme (drMM) survives and remains competent at least for 24 hours in the presence of human recombinant bone morphogenetic protein 7 (hrBMP7) and human recombinant fibroblast growth factor 2 (hrFGF2), and can assemble segmented nephrons when induced ex vivo. These findings provide a novel efficient method for the viral transduction of competent MM cells.…”

mentioning

confidence: 99%

Functional Genetic Targeting of Embryonic Kidney Progenitor Cells Ex Vivo

Junttila

Saarela

Halt

et al. 2015

Journal of the American Society of Nephrology

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The embryonic mammalian metanephric mesenchyme (MM) is a unique tissue because it is competent to generate the nephrons in response to Wnt signaling. An ex vivo culture in which the MM is separated from the ureteric bud (UB), the natural inducer, can be used as a classic tubule induction model for studying nephrogenesis. However, technological restrictions currently prevent using this model to study the molecular genetic details before or during tubule induction. Using nephron segment-specific markers, we now show that tubule induction in the MM ex vivo also leads to the assembly of highly segmented nephrons. This induction capacity was reconstituted when MM tissue was dissociated into a cell suspension and then reaggregated (drMM) in the presence of human recombinant bone morphogenetic protein 7/human recombinant fibroblast growth factor 2 for 24 hours before induction. Growth factor-treated drMM also recovered the capacity for organogenesis when recombined with the UB. Cell tracking and time-lapse imaging of chimeric drMM cultures indicated that the nephron is not derived from a single progenitor cell. Furthermore, viral vector-mediated transduction of green fluorescent protein was much more efficient in dissociated MM cells than in intact mesenchyme, and the nephrogenic competence of transduced drMM progenitor cells was preserved. Moreover, drMM cells transduced with viral vectors mediating Lhx1 knockdown were excluded from the nephric tubules, whereas cells transduced with control vectors were incorporated. In summary, these techniques allow reproducible cellular and molecular examinations of the mechanisms behind nephrogenesis and kidney organogenesis in an ex vivo organ culture/organoid setting.

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“…6 A-A' and G). High expression of X. tropicalis igfbp2 was detected in PT1-3, DT1-2, and CT, and was Raciti et al, (2008) detectable in DT1-2 (Fig. 6 C-D' and G).…”

Section: Expression Of Igfbps In the Pronephric Kidneymentioning

confidence: 95%

Characterization of the insulin-like growth factor binding protein family in Xenopus tropicalis

Haramoto

Oshima²,

Takahashi³

et al. 2014

Int. J. Dev. Biol.

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The insulin-like growth factor binding protein (Igfbp) family consists of six members designated Igfbp1-6. Igfbps are involved in many vital biological functions. They physically interact with IGFs (IGF1 and IGF2) and act as carriers, thereby protecting IGFs from proteolytic degradation. Thus, they function as modulators of IGF activity. Furthermore, Igfbps have been reported to have IGF-independent activities. They interact with other proteins, including cell surface proteins, extracellular matrix proteins, and potentially intracellular molecules. In Xenopus tropicalis (X. tropicalis), only four igfbp genes (igfbp1, igfbp2, igfbp4, and igfbp5) have been identified, and their expression is not well characterized. We report that X. tropicalis genome lacks the igfbp3 and igfbp6 genes based on synteny analyses. We also examined the spatio-temporal expression patterns of igfbp genes in early X. tropicalis development. Expression analyses indicated that they are differentially expressed during early development. Each igfbp gene showed a characteristic spatial expression pattern. Except for igfbp5, they demonstrated overlapping expression in the pronephros. The Xenopus pronephros is composed of four domains (i.e., the proximal tubule, intermediate tubule, distal tubule, and connecting tubule). Our results showed that at least two igfbp genes are co-expressed in all pronephric domains, suggesting that redundant functions of igfbp genes are required in early pronephric kidney development.

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Organization of the pronephric kidney revealed by large-scale gene expression mapping

Cited by 99 publications

References 93 publications

<em>Daphnia magna</em> and<em> Xenopus laevis</em> as in vivo models to probe toxicity and uptake of quantum dots functionalized with gH625

<em>Daphnia magna</em> and<em> Xenopus laevis</em> as in vivo models to probe toxicity and uptake of quantum dots functionalized with gH625

Functional Genetic Targeting of Embryonic Kidney Progenitor Cells Ex Vivo

Characterization of the insulin-like growth factor binding protein family in Xenopus tropicalis

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