Organ printing: computer-aided jet-based 3D tissue engineering

Mironov, Vladimir; Boland, Thomas; Trusk, Thomas C.; Forgács, Gabor; Markwald, Roger R.

doi:10.1016/s0167-7799(03)00033-7

Cited by 1,129 publications

(681 citation statements)

References 25 publications

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“…In addition, rapid prototyping technology has successfully been applied for computer-aided deposition of cells in gels to create 3D tissue constructs (13,14). We suggest that cell aggregates may be used as drops of ''bio-ink,'' which, upon implantation or ''printing'' into a scaffold (''bio-paper''), have the ability to fuse into 3D organ structures (15)(16)(17).…”

mentioning

confidence: 99%

Engineering biological structures of prescribed shape using self-assembling multicellular systems

Jakab

Neagu

Mironov

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

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350

241

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Self-assembly is a fundamental process that drives structural organization in both inanimate and living systems. It is in the course of self-assembly of cells and tissues in early development that the organism and its parts eventually acquire their final shape. Even though developmental patterning through self-assembly is under strict genetic control it is clear that ultimately it is physical mechanisms that bring about the complex structures. Here we show, both experimentally and by using computer simulations, how tissue liquidity can be used to build tissue constructs of prescribed geometry in vitro. Spherical aggregates containing many thousands of cells, which form because of tissue liquidity, were implanted contiguously into biocompatible hydrogels in circular geometry. Depending on the properties of the gel, upon incubation, the aggregates either fused into a toroidal 3D structure or their constituent cells dispersed into the surrounding matrix. The model simulations, which reproduced the experimentally observed shapes, indicate that the control parameter of structure evolution is the aggregate-gel interfacial tension. The model-based analysis also revealed that the observed toroidal structure represents a metastable state of the cellular system, whose lifetime depends on the magnitude of cell-cell and cell-matrix interactions. Thus, these constructs can be made long-lived. We suggest that spherical aggregates composed of organ-specific cells may be used as ''bio-ink'' in the evolving technology of organ printing.

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mentioning

confidence: 99%

Engineering biological structures of prescribed shape using self-assembling multicellular systems

Jakab

Neagu

Mironov

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

350

241

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“…41 In this process, standard or modified printers are used where paper and ink are replaced with substrate (usually polymer made) and cell-laden medium respectively. 42 In all of the hydrogel-based building block fabrication techniques that have been discussed so far in this section, the building blocks are first fabricated and then they will be organized in specific geometry using directed assembly. In contrast, in printing method, both steps of fabrication and assembly of hydrogels are merged.…”

Section: Directed Assembly Of Cell-laden Hydrogels For Engineering Timentioning

confidence: 99%

Directed assembly of cell-laden hydrogels for engineering functional tissues

Kachouie

Bae

et al. 2010

Organogenesis

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“…Just as advances in microfabrication have spurred technical developments in bioengineering, advances in printing technology are translating into biological applications [5,6]. Inkjet nozzles can eject a drop of ink on to a surface with relatively high (20-30 µm) accuracy.…”

Section: New Approaches Control the Placement Of Living Cellsmentioning

confidence: 99%

“…Although EHDJ has not yet been tested for this purpose, other printing technologies have been used to develop new methods in three-dimensional tissue engineering [5]. These kinds of 'organ printing' and three-dimensional tissue cultures are still in their infancy in terms of technical development and proof of concept, but they hold promise for understanding mechanisms that control migration of cells, outgrowth of neuronal processes and regeneration.…”

Section: Future Prospects For Printing Cellsmentioning

confidence: 99%

New ways to print living cells promise breakthroughs for engineering complex tissues in vitro

Withers

2006

Biochemical Journal

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The ability to control the placement of cells and the assembly of networks in vitro has tremendous potential for understanding the regulation of development as well as for generating artificial tissues. To date, most engineering tools that can place materials with precision are not compatible with the requirements of living cells, and so approaches to tissue engineering have focused on patterning substrates as a way of controlling cell growth rather than patterning cells directly. In this issue of Biochemical Journal, however, Eagles et al. adapt electrohydrodynamic printing technology to 'print' living cells from a neuronal cell line on to a substrate. The importance of this approach is that it has the potential for unprecedented control over the position of cells in culture by directly placing them, thus allowing for the systematic assembly of cell networks.

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Organ printing: computer-aided jet-based 3D tissue engineering

Cited by 1,129 publications

References 25 publications

Engineering biological structures of prescribed shape using self-assembling multicellular systems

Engineering biological structures of prescribed shape using self-assembling multicellular systems

Directed assembly of cell-laden hydrogels for engineering functional tissues

New ways to print living cells promise breakthroughs for engineering complex tissues in vitro

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