Organ Preservation Solutions in Transplantation: A Literature Review

Sousa, Samara Guilhermina de; Silva, Giulia Vitória Nascimento da; Rodrigues, Ana Maria Costa; Silva, Tereza Maria Meireles Fernandes da; Costa, Felipe Cruz; Silva, Amanda Freitas Teixeira da; Macedo, Bernardo Felipe Santana de; Brito, Marcus Vinicius Henriques

doi:10.6002/ect.2020.0506

Cited by 9 publications

(2 citation statements)

References 53 publications

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“…Ringer’s lactate solution was chosen as the base to develop the PSOP because of its extended clinical use as physiological fluid ( Singh and Davis, 2018 ; de Sousa et al, 2021 ). Mannitol was added as an impermeant agent to prevent cell swelling, one of the main requirements for preservation solutions ( Nicholson and Hosgood, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Development of a Novel Perfusable Solution for ex vivo Preservation: Towards Photosynthetic Oxygenation for Organ Transplantation

Veloso-Giménez

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et al. 2021

Front. Bioeng. Biotechnol.

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Oxygen is the key molecule for aerobic metabolism, but no animal cells can produce it, creating an extreme dependency on external supply. In contrast, microalgae are photosynthetic microorganisms, therefore, they are able to produce oxygen as plant cells do. As hypoxia is one of the main issues in organ transplantation, especially during preservation, the main goal of this work was to develop the first generation of perfusable photosynthetic solutions, exploring its feasibility for ex vivo organ preservation. Here, the microalgae Chlamydomonas reinhardtii was incorporated in a standard preservation solution, and key aspects such as alterations in cell size, oxygen production and survival were studied. Osmolarity and rheological features of the photosynthetic solution were comparable to human blood. In terms of functionality, the photosynthetic solution proved to be not harmful and to provide sufficient oxygen to support the metabolic requirement of zebrafish larvae and rat kidney slices. Thereafter, isolated porcine kidneys were perfused, and microalgae reached all renal vasculature, without inducing damage. After perfusion and flushing, no signs of tissue damage were detected, and recovered microalgae survived the process. Altogether, this work proposes the use of photosynthetic microorganisms as vascular oxygen factories to generate and deliver oxygen in isolated organs, representing a novel and promising strategy for organ preservation.

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Section: Discussionmentioning

confidence: 99%

Development of a Novel Perfusable Solution for ex vivo Preservation: Towards Photosynthetic Oxygenation for Organ Transplantation

Veloso-Giménez

Escamilla

Necuñir

et al. 2021

Front. Bioeng. Biotechnol.

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“…In this model, pressure conditions require to be adapted in accordance with the size of the considered intestinal segment, and this aspect will require further adaptations in order to perfection this model. Literature studies demonstrated that UW solution provides better conservation of patches through prolonged ischemia compared to other solutions [54]. Further studies are needed to assess the glucose absorption trough perfusion of higher glucose concentrations through the intraluminal line.…”

Section: Plos Onementioning

confidence: 99%

Swine intestinal segment perfusion model for the evaluation of nutrients bioaccessibility

et al. 2023

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Nutrition science requires more science-based evidences for the development of effective functional diets. To reduce animals for experimental purposes innovative reliable and informative models, simulating the complex intestinal physiology, are needed. The aim of this study was to develop a swine duodenum segment perfusion model for the evaluation of nutrient bioaccessibility and functionality across time. At the slaughterhouse, one sow intestine was harvested following Maastricht criteria for organ donation after circulatory death (DCD) for transplantation purposes. Duodenum tract was isolated and perfused in sub-normothermic conditions with heterologous blood after cold ischemia induction. Duodenum segment perfusion model was maintained under controlled pressure conditions through extracorporeal circulation for 3 hours. Blood samples from extracorporeal circulation and luminal content samples were collected at regular intervals for the evaluation of glucose concentration by glucometer, minerals (Na+, Ca2+, Mg2+, K+) by ICP-OES, lactate-dehydrogenase and nitrite oxide by spectrophotometric methods. Dacroscopic observation showed peristaltic activity caused by intrinsic nerves. Glycemia decreased over time (from 44.00±1.20 mg/dL to 27.50±0.41; p < 0.01), suggesting glucose utilization by the tissue confirming the organ viability in line with histological examinations. At the end of the experimental period, intestinal mineral concentrations were lower than their level in blood plasma suggesting their bioaccessibility (p < 0.001). A progressive increase of LDH concentration over time was observed in the luminal content probably related to a loss of viability (from 0.32±0.02 to 1.36±0.02 OD; p < 0.05) confirmed by histological findings that revealed a de-epithelization of the distal portion of duodenum. Isolated swine duodenum perfusion model satisfied the criteria for studying bioaccessibility of nutrients, offering a variety of experimental possibilities in line with 3Rs principle.

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Protection of rat artery grafts from tissue damage by voltage-applied supercooling

et al. 2022

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Organ Preservation Solutions in Transplantation: A Literature Review

Cited by 9 publications

References 53 publications

Development of a Novel Perfusable Solution for ex vivo Preservation: Towards Photosynthetic Oxygenation for Organ Transplantation

Development of a Novel Perfusable Solution for ex vivo Preservation: Towards Photosynthetic Oxygenation for Organ Transplantation

Swine intestinal segment perfusion model for the evaluation of nutrients bioaccessibility

Protection of rat artery grafts from tissue damage by voltage-applied supercooling

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