Orbitide Composition of the Flax Core Collection (FCC)

Burnett, Peta-Gaye G.; Olivia, Clara Marisa; Okinyo-Owiti, Denis P.; Reaney, Martin J. T.

doi:10.1021/acs.jafc.6b02035

Cited by 9 publications

(11 citation statements)

References 42 publications

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“…Hence, orbitide concentrations of 1 : 3 : 8 observed in ‘Hollandia’ and ‘Z 11637’ deviated from the 1:1:1 ratio expressed in ‘CDC Bethune’. These findings were consistent regardless of harvest location (Saskatoon, SK or Morden, MB) and year (2009, 2010, 2011) and corroborate observations made by Burnett et al [ 23 ] (Table 1 ). Low level detection of 1 and 3 in ‘Hollandia’ and ‘Z 11637’ resulted from flaxseed samples that were contaminated with one or more other accessions within the FCC.…”

Section: Resultssupporting

confidence: 91%

“…2b ). Stefanowicz [ 26 ], Okinyo-Owiti et al [ 20 ], and Burnett et al [ 23 ] have reported similar observations for orbitide content of fresh flaxseed extracts. Unoxidized flaxseed extracts from ‘Hollandia’ and ‘Z 11637’ contained known orbitides 7 , 10 , 14 , and 15 as found in ‘CDC Bethune’.…”

Section: Resultsmentioning

confidence: 59%

“…Recently, orbitide compositions of the flax core collection (FCC) harvested in 2009 were determined using high-performance liquid chromatography with reversed-phase monolithic HPLC columns and diode array detection (HPLC-DAD) [ 23 ]. The FCC is a subset of the Canadian flax world collection curated by Plant Gene Resources of Canada (PGRC) comprising 381 accessions [ 24 ].…”

Section: Resultsmentioning

confidence: 99%

“…Such contamination is not uncommon in field plot studies. In 2009, mean total orbitide contents of ‘Hollandia’, ‘Z 11637’ and ‘CDC Bethune’ were 139.2, 106.9 (Table 1 ) and 196.9 mAU [ 23 ]. Similar total orbitide concentrations in ‘Hollandia’ and ‘Z 11637’ were observed in 2010 and 2011 with mean 2010 values being lower probably due to poor seed quality from the Saskatoon plots that year.…”

Section: Resultsmentioning

confidence: 99%

“…Overall, the sum of apparent orbitide concentrations of 1 , 3 , and 8 (encoded by LINUSORB B ), hereafter refered to as [ 1 , 3 , 8 ], were lower than those of 6 , 13 , and 17 (encoded by LINUSORB A ), hereafter refered to as [ 6 , 13 , 17 ]. The mean apparent concentration ratio of [ 1 , 3 , 8 ] to [ 6 , 13 , 17 ] observed in accessions ‘Hollandia’ and ‘Z 11637’ over three growing seasons was 0.7 ± 0.2, while that observed in ‘CDC Bethune’ in 2009 was 1.9 ± 0.1 [ 23 ]. These ratios were consistent regardless of harvest location and reflected the apparent low total orbitide expression of these accessions.…”

Section: Resultsmentioning

confidence: 99%

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Novel flax orbitide derived from genetic deletion

et al. 2018

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BackgroundFlaxseed orbitides are homodetic plant cyclic peptides arising from ribosomal synthesis and post-translation modification (N to C cyclization), and lacking cysteine double bonds (Nat Prod Rep 30:108-160, 2013). Screening for orbitide composition was conducted on the flax core collection (FCC) grown at both Saskatoon, Saskatchewan and Morden, Manitoba over three growing seasons (2009-2011). Two flax (Linum usitatissimum L.) accessions ‘Hollandia’ (CN 98056) and ‘Z 11637’ (CN 98150) produce neither [1−9-NαC]-linusorb B2 (3) nor [1−9-NαC]-linusorb B3 (1). Mass spectrometry was used to identify novel compounds and elucidate their structure. NMR spectroscopy was used to corroborate structural information.ResultsExperimental findings indicated that these accessions produce a novel orbitide, identified in three oxidation states having quasimolecular ion peaks at m/z 1072.6 (18), 1088.6 (19), and 1104.6 (20) [M + H]+ corresponding to molecular formulae C57H86N9O9S, C57H86N9O10S, and C57H86N9O11S, respectively. The structure of 19 was confirmed unequivocally as [1−9-NαC]-OLIPPFFLI. PCR amplification and sequencing of the gene coding for 18, using primers developed for 3 and 1, identified the putative linear precursor protein of 18 as being comprised of the first three amino acid residues of 3 (MLI), four conserved amino acid residues of 3 and/or 1 (PPFF), and the last two residues of 1 (LI).ConclusionComparison of gene sequencing data revealed that a 117 base pair deletion had occurred that resulted in truncation of both 3 and 1 to produce a sequence encoding for the novel orbitide precursor of 18. This observation suggests that repeat units of flax orbitide genes are conserved and suggests a novel mechanism for evolution of orbitide gene diversity. Orbitides 19 and 20 contain MetO and MetO2, respectively, and are not directly encoded, but are products of post-translation modification of Met present in 18 ([1−9-NαC]-MLIPPFFLI).Electronic supplementary materialThe online version of this article (10.1186/s12870-018-1303-8) contains supplementary material, which is available to authorized users.

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Section: Resultssupporting

confidence: 91%