Orally administered lactoferrin restores humoral immune response in immunocompromised mice

Artym, Jolanta; Zimecki, Michał; Paprocka, Maria; Kruzel, Marian L.

doi:10.1016/s0165-2478(03)00102-0

Cited by 68 publications

(58 citation statements)

References 41 publications

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“…Additionally, bLF shows inhibitory effects as well as upregulatory effects on immune cells. Oral administration of bLF increased neutrophil phagocytic activity in FIV-positive cats [27], stimulated renewal of lymphocytes in drug-induced immunocompromised mice [1] and increased the percentage of CD4+ cell count significantly in HIV-infected children [38], whereas glucocorticoids completely impaired both T cell responses and neutrophil functions [10]. These studies suggest that clinical application of LF, which has immunomodulatory properties on immune system to maintain the homeostatic function of immune cells, may be advantageous to immunosuppressed FIV-positive cats.…”

Section: Cycle Numbermentioning

confidence: 99%

Effect of Bovine Lactoferrin on Functions of Activated Feline Peripheral Blood Mononuclear Cells During Chronic Feline Immunodeficiency Virus Infection

et al. 2008

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ABSTRACT. Feline immunodeficiency virus (FIV) infection is characterized by chronic overactivation of immune and inflammatory system, resulting in anergic state and dysfunction of immune cells. Lactoferrin (LF), a glycoprotein present in exocrine secretions and neutrophils, plays an important role in host defense system. Our previous study showed that oral administration of bovine LF (bLF) suppressed oral inflammation, improved the clinical symptoms and decreased serum γ-globulin as a marker of inflammation in FIV-infected cats with intractable stomatitis. The anti-inflammatory effect was partly involved in regulation of neutrophil function by bLF. In this study, to clarify the relationship between anti-inflammatory effects of bLF and peripheral blood mononuclear cells (PBMC), we examined the effect of bLF on proliferation, cell cycle progression and cytokine expression in mitogen-activated PBMC. MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay showed that bLF inhibited the concanavalin A (ConA)-induced cell proliferation in FIVinfected cats with the asymptomatic carrier and AIDS-related complex (ARC) phase. Bovine LF restored ConA-induced cell cycle progression and resulted in suppression of the induced apoptosis in feline PBMC. Real-time RT-PCR showed that bLF suppressed ConAinduced expression of interferon-gamma and interleukin-2 in cells of the ARC group regardless of the time of its addition to the medium. These results suggest the hypothesis that therapy with bLF may have the potential to improve and protect functions of overactivated lymphocytes by modulating the cell proliferation, cell cycle and cytokines expression in cats in terminal stage of FIV infection. KEY WORDS: anti-inflammatory activity, bovine lactoferrin, FIV-infected cat, persistent immune activation.

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Section: Cycle Numbermentioning

confidence: 99%

Effect of Bovine Lactoferrin on Functions of Activated Feline Peripheral Blood Mononuclear Cells During Chronic Feline Immunodeficiency Virus Infection

et al. 2008

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“…In the same experimental model, it was demonstrated that Lf strongly elevated the pool of CD3+ T cells and CD4+ T cell content. Lf, given orally to CP-immunosuppressed mice, could reconstitute a T cell-mediated immune response by renewal of the T cell pool [97]. A recent study 2554 D. Legrand et al Lactoferrin modulates immune and infl ammatory responses has demonstrated that oral Lf administration prevents body weight loss and increases cytokine responses during herpes simplex virus type 1 infection of mice [98].…”

Section: Pro-infl Ammatory Propertiesmentioning

confidence: 99%

Lactoferrin

Legrand

Elass

Carpentier

et al. 2005

Cell. Mol. Life Sci.

352

117

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Lactoferrin is an iron-binding glycoprotein of the transferrin family. Abundant expression and secretion of lactoferrin, in particular in milk and fluids of the digestive tract, are related to its implication in the first line of host defense. Lactoferrin is also a prominent component of the secondary granules of neutrophils (PMNs) and is released in infected tissues and blood during the inflammatory process. In addition to its direct antimicrobial properties, the abilities of lactoferrin to regulate the immune response and to protect against infection and septic shock have been described in numerous in vitro and in vivo studies. Although the cellular and molecular mechanisms that account for the modulation of the inflammatory and immune responses by lactoferrin are not yet totally elucidated, many are now established. At the cellular level, lactoferrin modulates the migration, maturation and function of immune cells. At the molecular level and in addition to iron binding, interactions of lactoferrin with a plethora of compounds, either soluble or membrane molecules, account for its modulatory properties. This paper reviews our current understanding of the cellular and molecular mechanisms that explain the regulatory properties of lactoferrin in host defence.

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“…Recently, some food proteins have been demonstrated to modulate immune systems. Artym et al [7] found that oral administration of lactoferrin improved the humoral immune response in immunodeficient mice. Hata et al [8] isolated a peptide with mitogenic activity toward mouse spleen cells from αs1-casein digested with trypsin, and identified the peptide casein phosphopeptide (CPP), which corresponds to residues 59 to 79 of αs1-casein.…”

Section: Introductionmentioning

confidence: 99%

Soybean protein fraction digested with neutral protease preparation, “Peptidase R”, produced by Rhizopus oryzae, stimulates innate cellular immune system in mouse

Egusa

Otani

2009

International Immunopharmacology

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Orally administered lactoferrin restores humoral immune response in immunocompromised mice

Cited by 68 publications

References 41 publications

Effect of Bovine Lactoferrin on Functions of Activated Feline Peripheral Blood Mononuclear Cells During Chronic Feline Immunodeficiency Virus Infection

Effect of Bovine Lactoferrin on Functions of Activated Feline Peripheral Blood Mononuclear Cells During Chronic Feline Immunodeficiency Virus Infection

Lactoferrin

Soybean protein fraction digested with neutral protease preparation, “Peptidase R”, produced by Rhizopus oryzae, stimulates innate cellular immune system in mouse

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