Oral exposure of sulpiride promotes the proliferation of Brown‑Norway rat prostates

Zheng, Chao; Luo, Yongwei; Chen, Ying; Chen, Dingshi; Shao, Congcong; Huang, Dongyan; Zhu, Jing Yuan; Mao, Xiaoyan; Li, Lei; Sun, Zilong

doi:10.3892/etm.2020.8521

Cited by 3 publications

(3 citation statements)

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“…The BPH subjects had significantly higher levels of prolactin compared to the control subjects. This is probably because the levels of prolactin increase with increasing age (22). Also, estrogen stimulates the pituitary gland to release prolactin, which in turn stimulates prostate enlargement (3).…”

Section: Discussionmentioning

confidence: 99%

Serum Levels of Prostate Specific Antigen and Specific Reproductive Hormones Among Male Subjects with Benign Prostate Hyperplasia in Port Harcourt, Nigeria

Elechi-Amadi,

Ogregade,

Favour

et al. 2024

AIM

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Benign prostate hyperplasia (BPH) is a medical condition in elderly men in which there is proliferation and enlargement of the prostate gland. This study evaluated the levels of male reproductive hormones among subjects with BPH. The study involved 150 subjects aged 40 years and above, comprising 80 BPH subjects attending the urology clinic and 70 control subjects. Five millilitres (5ml) of venous blood were collected from each subject into plain bottles for the determination of prostate-specific antigen (PSA), testosterone, prolactin, and estradiol, using the ELISA technique. The mean values of PSA (16.68±10.96 ng/ml), estradiol (71.03±18.56 pg/ml) and for the BPH subjects and prolactin (9.38±4.51 ng/ml) were significantly higher compared to the mean values of PSA (0.48±0.25ng/ml), estradiol (51.33±7.13npg/ml) and prolactin (6.92±1.93ng/ml) of the control subjects. However, the mean testosterone value of the BPH subjects (5.02±1.93 ng/ml) was significantly lower than the mean value for the control (6.57±3.48ng/ml). The BPH who used to consume alcohol had higher PSA (24.26±8.33ng/ml) and testosterone (7.68±3.41ng/ml) compared to the PSA (16.34±3.22ng/ml) and testosterone (4.95±3.62ng/ml) of those who never consumed alcohol. The BPH had significantly altered hormone parameters as well as raised PSA levels. Including hormonal parameters in diagnosing and managing BPH could be an important consideration in our population.

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Section: Discussionmentioning

confidence: 99%

Serum Levels of Prostate Specific Antigen and Specific Reproductive Hormones Among Male Subjects with Benign Prostate Hyperplasia in Port Harcourt, Nigeria

Elechi-Amadi,

Ogregade,

Favour

et al. 2024

AIM

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“…Sections were incubated with the following primary antibodies for immunostaining: gamma H2Ax and Poly (ADP-ribose) polymerase 1 (PARP1), proliferating cell nuclear antigen (PCNA) [ 50 ], caspase-3, TGF-β1 [ 51 ], and alpha smooth muscle actin (α-SMA) [ 52 ] as presented in Table 2 . Then, they were left in phosphate-buffered saline (PBS) at 4 °C overnight before adding the universal secondary anti-mouse IgGκ (sc-516102, Santa Cruz, CA, USA) or anti-rabbit IgG (sc-2357, Santa Cruz) for 30 min to localize the primary antibody binding followed by washing again with PBS.…”

Section: Methodsmentioning

confidence: 99%

Origanum majorana L. Extract Attenuated Benign Prostatic Hyperplasia in Rat Model: Effect on Oxidative Stress, Apoptosis, and Proliferation

et al. 2022

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Benign prostatic hyperplasia (BPH) is a widespread androgenic illness influencing elderly men. It is distinguished by prostatic epithelial and stromal muscle cell proliferation. Inflammation, oxidative stress, and apoptosis have all been interrelated to the development of BPH. Marjoram (Origanum majorana L.) is a herb with reported antiproliferative, proapoptotic, and antioxidative properties, which have not yet been studied in relation to BPH. Consequently, in this work, an ethanolic extract of O. majorana was prepared in two doses (250 and 500 mg/kg/day) to be injected into castrated rats after induction of a testosterone-BPH model. Testosterone propionate (TP) was subcutaneously injected (0.5 mg/kg/day) for one week after castration to induce BPH. Forty adult Wistar male rats were randomly allocated into five groups: control, BPH model, high and low O. majorana doses (250, 500 mg/kg/day), and finasteride (FN) (0.8 mg/kg/day) as a positive control. Treatment was continued with drugs/normal saline for 28 days. Rat’s body and prostate were weighed, prostate index (PI) and % of prostate growth inhibition were calculated, serum dihydrotestosterone (DHT), prostatic content of superoxide dismutase (SOD), catalase (CAT), total antioxidant capacity (TAC), and malondialdehyde (MDA), DN damage, histopathological changes, immune expression of proliferating cell nuclear antigen (PCNA), caspase-3, α-SMA, and TGF-β1 were assessed. In addition, molecular quantitative PCR and ELISA analyses were performed to identify the expression of mRNAs and related proteins of both caspase-3 and TGF-β1 in prostate tissue from O. majorana-treated and untreated groups. Rats with BPH had significantly higher prostate weights and PI, higher DHT, DNA damage (8-hydroxyguanine, 8-OH-dG), and MDA levels with prominent PCNA, α-SMA, and TGF-β expression, but lower SOD, CAT, and TAC activity and caspase-3 expression. O. majorana (250 and 500 mg/kg/day)-treated groups revealed a decrease in prostate weights and PI, lower levels of DHT, suppressed oxidative stress, reduced tissue proliferation and fibrosis, and restored antioxidant and proapoptotic activity. Additionally, quantitative PCR and ELISA analysis showed that treatment with O. majorana significantly upregulated the expression of caspase-3 and downregulated the expression of TGF-β in prostate tissues of BPH rats. The data were confirmed by the immunohistological reactivity of these targeted markers in the prostate tissues. These effects were more significant with O. majorana 500 mg/mL/rat. In conclusion, the current study indicates the efficient use of O. majorana in the treatment of testosterone-induced BPH through its antiproliferative, proapoptotic, and antioxidative mechanisms.

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“…Значение секреторных продуктов придаточных половых желез многообразно. Секрет придаточных половых желез увеличивает объем эякулята (на долю секрета добавочных желез мужской половой системы приходится около 95% общего объема эякулята, а на долю сперматозоидов приходится не более 5%), способствует продвижению сперматозоидов по женским половым путям, промывает мочеполовой канал, содержащиеся в составе секрета придаточных половых желез биологически активные вещества (например, простагландины) вызывают усиление сокращения гладких мышечных клеток стенок женских половых путей, что также ускоряет перемещение сперматозоидов по половым путям [13,14,41,48,54,56,61,67].…”

Section: Introductionunclassified

Seminal Vesicles and Bulbourethral Glands of Mammals: Morphology, Physiology, Ecology, Action of Extreme Destabilizing Factors

Shevlyuk

Ryskulov

2021

Žurnal anatomii i gistopatologii

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In mammals, the adnexal sex glands are represented by seminal vesicles, the prostate gland, urethral and bulbourethral glands, as well as glands that coagulate sperm and ampullary glands. The secret of the accessory genital glands increases the volume of the ejaculate (the share of secretions of these glands accounts for about 95% of the volume of ejaculate) promotes sperm, causes increased contraction of smooth muscle cells in the walls of the female genital tract.The purpose of this review is to analyze the morphofunctional organization of seminal vesicles and bulbourethral glands of mammalian animals and humans.The presence or absence of seminal vesicles is a species-specific feature. Among mammals, seminal vesicles are well developed in some rodents, insectivores, a number of domestic animals (cattle, pigs), and primates. These glands are absent in cloacae, marsupials, some carnivores, a number of insectivores, artiodactyls. Bulbourethral glands are well developed in rodents, bats, primates, and some ungulates.In the wall of the seminal vesicles, the mucous, muscular and outer membranes are isolated. The epithelium of the secretory parts is pseudomultitial, the interstitium is represented by loose fibrous connective tissue and a significant number of smooth muscle cells. In the wall of the bulbourethral glands, the mucosa and adventitial membrane are isolated. The secretory end sections of the bulbourethral glands are lined with a single-layer single-row epithelium, glandular cells produce a mucosal or mixed secret. The seminal vesicles and bulbourethral glands are androgen-dependent glands. In species with a seasonal pattern of reproduction, their morphofunctional characteristics undergo significant changes during the circannual rhythm of reproduction.The epithelium of seminal vesicles and bulbourethral glands is very sensitive to the action of various adverse factors (heavy metal compounds, organic xenobiotics, electromagnetic radiation, ultrasound, etc.). When exposed to various negative factors in the adnexal glands, a complex of changes occurs (edema of connective tissue and epithelium, decreased secretory activity of epithelial cells, desynchronization of the secretory cycle, desquamation of glandular epithelial cells, proliferation of interstitial connective tissue).There is a lack of information on many aspects of the characteristics of the adnexal glands of the male reproductive system, primarily on the morphology and physiology of the adnexal glands of animals in natural ecosystems, on the ultrastructural and immunohistochemical characteristics of these glands, as well as on the mechanisms of regulation of morphofunctional rearrangements of the adnexal glands during seasonal reproduction rhythms, in the conditions of adaptation to various negative influences.

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Oral exposure of sulpiride promotes the proliferation of Brown‑Norway rat prostates

Cited by 3 publications

References 77 publications

Serum Levels of Prostate Specific Antigen and Specific Reproductive Hormones Among Male Subjects with Benign Prostate Hyperplasia in Port Harcourt, Nigeria

Serum Levels of Prostate Specific Antigen and Specific Reproductive Hormones Among Male Subjects with Benign Prostate Hyperplasia in Port Harcourt, Nigeria

Origanum majorana L. Extract Attenuated Benign Prostatic Hyperplasia in Rat Model: Effect on Oxidative Stress, Apoptosis, and Proliferation

Seminal Vesicles and Bulbourethral Glands of Mammals: Morphology, Physiology, Ecology, Action of Extreme Destabilizing Factors

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