Oral epithelial cells transplanted on to corneal surface tend to adapt to the ocular phenotype

Gaddipati, Subhash; Muralidhar, R; Sangwan, Veena; Mariappan, Indumathi; Vemuganti, Geeta K.; Balasubramanian, D.

doi:10.4103/0301-4738.109517

Cited by 37 publications

(28 citation statements)

References 8 publications

(9 reference statements)

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“…Other alternative scaffolds are still in an experimental phase and have yet to be validated in humans. COMET and human conjunctival epithelial cell transplantation have both been successfully performed in selected patients [ 43 , 221 , 223 – 232 , 235 ]. However, as iPSCs get widespread attention in many medical disciplines, it is believed that this autologous cell population will play a prominent role in LSCD treatment in the coming years.…”

Section: Resultsmentioning

confidence: 99%

“…Oral Mucosal Epithelial Cells (OMECs) are cultured on a HAM until a stratified epithelium is attained and then transplanted. The construct mimics the corneal epithelium as transplanted stem cells maintain their stemness at the ectopic site, and OMECs acquire corneal epithelial-like markers such as CK3, CK19, Ki-67, p63, p75, and cornea-specific PAX6 and CK12 [ 221 – 223 ]. COMET has been successful (i.e., regenerating a totally epithelized, stable, and avascular corneal surface) in patients with severe total LSCD [ 221 , 223 – 232 ].…”

Section: Alternative Cell Populationsmentioning

confidence: 99%

“…The construct mimics the corneal epithelium as transplanted stem cells maintain their stemness at the ectopic site, and OMECs acquire corneal epithelial-like markers such as CK3, CK19, Ki-67, p63, p75, and cornea-specific PAX6 and CK12 [ 221 – 223 ]. COMET has been successful (i.e., regenerating a totally epithelized, stable, and avascular corneal surface) in patients with severe total LSCD [ 221 , 223 – 232 ]. However, transplanted cultivated sheets are not completely identical to in vivo corneal epithelium, which leads to a variable degree of in vivo keratinization and stratification (up to 12 cell layers) [ 221 , 228 ].…”

Section: Alternative Cell Populationsmentioning

confidence: 99%

See 2 more Smart Citations

Limbal Stem Cell Deficiency: Current Treatment Options and Emerging Therapies

Haagdorens

Acker

Gerwen

et al. 2015

Stem Cells International

124

118

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Severe ocular surface disease can result in limbal stem cell deficiency (LSCD), a condition leading to decreased visual acuity, photophobia, and ocular pain. To restore the ocular surface in advanced stem cell deficient corneas, an autologous or allogenic limbal stem cell transplantation is performed. In recent years, the risk of secondary LSCD due to removal of large limbal grafts has been significantly reduced by the optimization of cultivated limbal epithelial transplantation (CLET). Despite the great successes of CLET, there still is room for improvement as overall success rate is 70% and visual acuity often remains suboptimal after successful transplantation. Simple limbal epithelial transplantation reports higher success rates but has not been performed in as many patients yet. This review focuses on limbal epithelial stem cells and the pathophysiology of LSCD. State-of-the-art therapeutic management of LSCD is described, and new and evolving techniques in ocular surface regeneration are being discussed, in particular, advantages and disadvantages of alternative cell scaffolds and cell sources for cell based ocular surface reconstruction.

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Section: Resultsmentioning

confidence: 99%

Section: Alternative Cell Populationsmentioning

confidence: 99%

Section: Alternative Cell Populationsmentioning

confidence: 99%

See 1 more Smart Citation

Limbal Stem Cell Deficiency: Current Treatment Options and Emerging Therapies

Haagdorens

Acker

Gerwen

et al. 2015

Stem Cells International

124

118

View full text Add to dashboard Cite

show abstract

“…Engineering cell sheets with Food and Drug Administration (FDA) approved products is a difficult task, but their harvesting requires specific protocols and growing surfaces. Different methodologies were developed to facilitate corneal-like cell sheet harvesting: fibrin glue [72], contact lenses [73], amniotic membranes [74], thermo-responsive surfaces [55], and dispase [65,75]. The last three methodologies were used for human studies.…”

Section: Carrier For Cell Sheet Graftingmentioning

confidence: 99%

Clinical Trials of Limbal Stem Cell Deficiency Treated with Oral Mucosal Epithelial Cells

Oliva¹,

Bardag‐Gorce²,

Niihara³

2020

IJMS

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The corneal surface is an essential organ necessary for vision, and its clarity must be maintained. The corneal epithelium is renewed by limbal stem cells, located in the limbus and in palisades of Vogt. Palisades of Vogt maintain the clearness of the corneal epithelium by blocking the growth of conjunctival epithelium and the invasion of blood vessels over the cornea. The limbal region can be damaged by chemical burns, physical damage (e.g., by contact lenses), congenital disease, chronic inflammation, or limbal surgeries. The degree of limbus damage is associated with the degree of limbal stem cells deficiency (partial or total). For a long time, the only treatment to restore vision was grafting part of the healthy cornea from the other eye of the patient or by transplanting a cornea from cadavers. The regenerative medicine and stem cell therapies have been applied to restore normal vision using different methodologies. The source of stem cells varies from embryonic stem cells, mesenchymal stem cells, to induced pluripotent stem cells. This review focuses on the use of oral mucosa epithelial stem cells and their use in engineering cell sheets to treat limbal stem cell deficient patients.

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“…Anti-angiogenic therapy has been proposed as a method to prevent corneal neovascularization and improve the outcomes after transplantation with CAOMECS [ 36 ]. Thus far, 242 patients with LSCD have been reported as treated, with a success rate of 72% and a follow-up time of between one and 7.5 years [ 19 , 28 , 29 , 30 , 31 , 32 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 ].…”

Section: Cultured Autologous Oral Mucosal Epithelial Cell Sheetmentioning

confidence: 99%

Culture of Oral Mucosal Epithelial Cells for the Purpose of Treating Limbal Stem Cell Deficiency

Utheim

Khan

et al. 2016

JFB

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The cornea is critical for normal vision as it allows allowing light transmission to the retina. The corneal epithelium is renewed by limbal epithelial cells (LEC), which are located in the periphery of the cornea, the limbus. Damage or disease involving LEC may lead to various clinical presentations of limbal stem cell deficiency (LSCD). Both severe pain and blindness may result. Transplantation of cultured autologous oral mucosal epithelial cell sheet (CAOMECS) represents the first use of a cultured non-limbal autologous cell type to treat this disease. Among non-limbal cell types, CAOMECS and conjunctival epithelial cells are the only laboratory cultured cell sources that have been explored in humans. Thus far, the expression of p63 is the only predictor of clinical outcome following transplantation to correct LSCD. The optimal culture method and substrate for CAOMECS is not established. The present review focuses on cell culture methods, with particular emphasis on substrates. Most culture protocols for CAOMECS used amniotic membrane as a substrate and included the xenogeneic components fetal bovine serum and murine 3T3 fibroblasts. However, it has been demonstrated that tissue-engineered epithelial cell sheet grafts can be successfully fabricated using temperature-responsive culture surfaces and autologous serum. In the studies using different substrates for culture of CAOMECS, the quantitative expression of p63 was generally poorly reported; thus, more research is warranted with quantification of phenotypic data. Further research is required to develop a culture system for CAOMECS that mimics the natural environment of oral/limbal/corneal epithelial cells without the need for undefined foreign materials such as serum and feeder cells.

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Oral epithelial cells transplanted on to corneal surface tend to adapt to the ocular phenotype

Cited by 37 publications

References 8 publications

Limbal Stem Cell Deficiency: Current Treatment Options and Emerging Therapies

Limbal Stem Cell Deficiency: Current Treatment Options and Emerging Therapies

Clinical Trials of Limbal Stem Cell Deficiency Treated with Oral Mucosal Epithelial Cells

Culture of Oral Mucosal Epithelial Cells for the Purpose of Treating Limbal Stem Cell Deficiency

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