Oral biofilm elimination by combining iron-based nanozymes and hydrogen peroxide-producing bacteria

Wang, Yanqiu; Shen, Xinyu; Ma, Shang; Guo, Qianqian; Zhang, Wei; Cheng, Liang; Ding, Liming; Xu, Zhuobin; Jiang, Jing; Gao, Lizeng

doi:10.1039/c9bm01889a

Cited by 49 publications

(31 citation statements)

References 40 publications

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“… 35 – 37 Targeting EPS is a promising approach in biofilm control and recent studies have declared kinds of nanoparticles disrupting biofilm microenvironment, which allows disruption of the matrix. 38 – 40 Targeted therapy for dental caries is becoming more popular, 41 so a thorough understanding of the targeted EPS is a keystone. Structural features of polysaccharides like molecular weight may play an important role in various biological activities.…”

Section: Discussionmentioning

confidence: 99%

The vicK gene of Streptococcus mutans mediates its cariogenicity via exopolysaccharides metabolism

et al. 2021

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Streptococcus mutans (S. mutans) is generally regarded as a major contributor to dental caries because of its ability to synthesize extracellular polysaccharides (EPS) that aid in the formation of plaque biofilm. The VicRKX system of S. mutans plays an important role in biofilm formation. The aim of this study was to investigate the effects of vicK gene on specific characteristics of EPS in S. mutans biofilm. We constructed single-species biofilms formed by different mutants of vicK gene. Production and distribution of EPS were detected through atomic force microscopy, scanning electron microscopy and confocal laser scanning microscopy. Microcosmic structures of EPS were analyzed by gel permeation chromatography and gas chromatography-mass spectrometry. Cariogenicity of the vicK mutant was assessed in a specific pathogen-free rat model. Transcriptional levels of cariogenicity-associated genes were confirmed by quantitative real-time polymerase chain reaction. The results showed that deletion of vicK gene suppressed biofilm formation as well as EPS production, and EPS were synthesized mostly around the cells. Molecular weight and monosaccharide components underwent evident alterations. Biofilms formed in vivo were sparse and contributed a decreased degree of caries. Moreover, expressional levels of genes related to EPS synthesis were down-regulated, except for gtfB. Our report demonstrates that vicK gene enhances biofilm formation and subsequent caries development. And this may due to its regulations on EPS metabolism, like synthesis or microcosmic features of EPS. This study suggests that vicK gene and EPS can be considered as promising targets to modulate dental caries.

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Section: Discussionmentioning

confidence: 99%

The vicK gene of Streptococcus mutans mediates its cariogenicity via exopolysaccharides metabolism

et al. 2021

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“…For instance, the Fe 3 O 4 nanoparticles with peroxidase-like activity enhanced oxidative cleavage of biofilm components in the presence of H 2 O 2 , which prevented biofilms formation and exterminated both planktonic bacteria and microbes residing within the biofilm [38]. In other interesting research, the combination of iron oxide or iron sulphide nanozymes and bacteria generating biogenic H 2 O 2 was used to eliminate oral biofilms [39]. Our results showed that the obtained Fe-and N-co-doped porous carbon materials possess similar, intriguing properties.…”

Section: Discussionmentioning

confidence: 99%

Interactions of Fe–N–S Co-Doped Porous Carbons with Bacteria: Sorption Effect and Enzyme-Like Properties

et al. 2020

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Carbon-based (nano)materials doped with transition metals, nitrogen and other heteroatoms are considered active heterogeneous catalysts in a wide range of chemical processes. Recently they have been scrutinized as artificial enzymes since they can catalyze proton-coupled electron transfer reactions vital for living organisms. Herein, interactions between Gram-positive and Gram-negative bacteria and either metal-free N and/or S doped or metal containing Fe−N−S co-doped porous carbons are studied. The Fe- and N-co-doped porous carbons (Fe−N−C) exhibit enhanced affinity toward bacteria as they show the highest adsorption capacity. Fe−N−C materials also show the strongest influence on the bacteria viability with visible toxic effect. Both types of bacteria studied reacted to the presence of Fe-doped carbons in a similar manner, showing a decrease in dehydrogenases activity in comparison to controls. The N-coordinated iron-doped carbons (Fe−N−C) may exhibit oxidase/peroxidase-like activity and activate O2 dissolved in the solution and/or oxygen-containing species released by the bacteria (e.g., H2O2) to yield highly bactericidal reactive oxygen species. As Fe/N/ and/or S-doped carbon materials efficiently adsorb bacteria exhibiting simultaneously antibacterial properties, they can be applied, inter alia, as microbiological filters with enhanced biofouling resistance.

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“…Impressively, the nFeS not only exhibited a broad spectrum to both Gram‐positive and Gram‐negative bacteria, [ 11 ] but also eliminated biofilm and intracellular bacteria. [ 12 ] Further studies revealed that the antibacterial activity was dependent on a ferroptosis‐like death in bacteria dominated by the released ferrous iron from nFeS under the condition without molecules that can chelate iron or resist oxidative damage. [ 13 ] Unfortunately, the antibacterial performance of nFeS dramatically decreased for bacteria like Escherichia coli or Staphylococcus aureus in nutrient‐rich media or physiological environment, making them equivocal for in vivo antibacterial therapy.…”

Section: Introductionmentioning

confidence: 99%

Metastable Iron Sulfides Gram‐Dependently Counteract Resistant Gardnerella Vaginalis for Bacterial Vaginosis Treatment

Fang

Gao

et al. 2022

Advanced Science

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Bacterial vaginosis (BV) is the most common vaginal infection found inwomen in the world. Due to increasing drug-resistance of virulent pathogen such as Gardnerella vaginalis (G. vaginalis), more than half of BV patients suffer recurrence after antibotics treatment. Here, metastable iron sulfides (mFeS) act in a Gram-dependent manner to kill bacteria, with the ability to counteract resistant G. vaginalis for BV treatment. With screening of iron sulfide minerals, metastable Fe 3 S 4 shows suppressive effect on bacterial growth with an order: Gram-variable G. vaginalis >Gram-negative bacteria>> Gram-positive bacteria. Further studies on mechanism of action (MoA) discover that the polysulfide species released from Fe 3 S 4 selectively permeate bacteria with thin wall and subsequently interrupt energy metabolism by inhibiting glucokinase in glycolysis, and is further synergized by simultaneously released ferrous iron that induces bactericidal damage. Such multiple MoAs enable Fe 3 S 4 to counteract G. vaginalis strains with metronidazole-resistance and persisters in biofilm or intracellular vacuole, without developing new drug resistance and killing probiotic bacteria. The Fe 3 S 4 regimens successfully ameliorate BV with resistant G. vaginalis in mouse models and eliminate pathogens from patients suffering BV. Collectively, mFeS represent an antibacterial alternative with distinct MoA able to treat challenged BV and improve women health.

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Oral biofilm elimination by combining iron-based nanozymes and hydrogen peroxide-producing bacteria

Abstract: Combining nanozymes and bacteria to eliminate dental biofilms in a mixed-species model.

Cited by 49 publications

References 40 publications

The vicK gene of Streptococcus mutans mediates its cariogenicity via exopolysaccharides metabolism

The vicK gene of Streptococcus mutans mediates its cariogenicity via exopolysaccharides metabolism

Interactions of Fe–N–S Co-Doped Porous Carbons with Bacteria: Sorption Effect and Enzyme-Like Properties

Metastable Iron Sulfides Gram‐Dependently Counteract Resistant Gardnerella Vaginalis for Bacterial Vaginosis Treatment

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