Oral administration of pharmacological doses of Vitamins C and E reduces reproductive fitness and impairs the ovarian and uterine functions of female mice

Tarı́n, Juan J.; Pérez-Albalá, Sonia; Pertusa, José; Cano, Antonio

doi:10.1016/s0093-691x(02)00636-2

Cited by 35 publications

(18 citation statements)

References 39 publications

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“…Antioxidant vitamins, alone or combined with other supplements, decrease embryonic mortality and improve birth outcomes (Cederberg et al 2001;Coffey and Britt 1993). However, the association between dietary antioxidant vitamins or their serum levels and birth outcomes is not conclusive in human or animal studies Pusateri et al 1999;Tamura et al 1997;Tarín et al 2002). In human, serum concentrations of vitamins A and E did not correlate with pregnancy outcome (Tamura et al 1997), and high doses of oral vitamin E during the first trimester of pregnancy was associated with decreased birth weight (Boskovic et al 2005).…”

Section: Discussionmentioning

confidence: 99%

“…In human, serum concentrations of vitamins A and E did not correlate with pregnancy outcome (Tamura et al 1997), and high doses of oral vitamin E during the first trimester of pregnancy was associated with decreased birth weight (Boskovic et al 2005). In mice, oral administration of pharmacological doses of vitamins C and E impaired the ovarian and uterine functions (Tarín et al 2002). In sows, injection of vitamin A at any stage of gestation failed to increase the litter size Pusateri et al 1999;Tamura et al 1997;Tarín et al 2002).…”

Section: Discussionmentioning

confidence: 99%

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Changes of vitamins A, E, and C and lipid peroxidation status of breeding and pregnant sheep during dry seasons on medium-to-low quality forages

Mohebbi‐Fani

Mirzaei

Nazifi

et al. 2011

Trop Anim Health Prod

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This study assessed the changes of plasma vitamin A, E, and C and the lipid peroxidation status of sheep during breeding and pregnancy under drought conditions. The study was conducted on 105 cross-bred fat tailed ewes, 3-5 years old with body condition scores (BCS) of 2.5 to 3.5. The ewes were grazing on medium-to-low quality forages during summer and low quality forages within the succeeding months and had ad libitum access to a mixture of alfalfa hay (40%) and wheat straw (60%) in the afternoons. From 3 weeks before breeding till 1 month after the introduction of rams, 300 g of barley grain/head/day was offered to the ewes and then the supplemental grain was reduced to 100 g/head/day. For better synchronization of estrus cycles in ewes, they were isolated from the rams for at least 2 months and then kept in close proximity of the rams for 1 week before the introduction of the rams to the ewe flock. Then, whole blood samples were collected on days 1, 7, 21, and 120 after ram introduction. Vitamins A, E, and C were measured in plasma. Malondialdehyde (MDA) was measured in the hemolysate as a biomarker of lipid peroxidation. Plasma progesterone (P4) was measured in the samples of day 120 for assessing pregnancy status of the ewes. Vitamins A and C showed continuous and significant declines (P < 0.05) through days 1 to 120. Vitamin E declined only during the first 21 days of the study and remained almost constant till day 120. MDA concentration increased significantly at day 21. An elevated concentration of MDA was also detected at day 120. The difference between days 21 and 120 was not significant (P > 0.05). A positive correlation between vitamins E and C was detected at day 120 (r = 0.349, P < 0.01). Age and BCS did not affect the patterns of changes. Assuming that the ewes with P4 concentrations ≥2.5 ng/ml were pregnant, 95 out of 105 ewes (90.5%) were pregnant at day 120 of the study. Under the conditions of the present study with medium-to-low quality pastures as the main sources of feed, ewes of various ages and body conditions may suffer from oxidative stress during breeding and pregnancy.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Changes of vitamins A, E, and C and lipid peroxidation status of breeding and pregnant sheep during dry seasons on medium-to-low quality forages

Mohebbi‐Fani

Mirzaei

Nazifi

et al. 2011

Trop Anim Health Prod

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“…However, this approach has significant long-term negative effects on ovarian and uterine function, leading to higher fetal death and resorptions and decreased litter frequency and size in treated animals (11). Whereas clinical translation of chronic antioxidant therapy for maintaining oocyte quality is therefore impractical, these studies tie the free radical theory of aging (12) to reduced oocyte quality in females of advanced reproductive age (13).…”

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confidence: 99%

Prevention of maternal aging-associated oocyte aneuploidy and meiotic spindle defects in mice by dietary and genetic strategies

Selesniemi

Lee

Muhlhauser

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

174

167

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Increased meiotic spindle abnormalities and aneuploidy in oocytes of women of advanced maternal ages lead to elevated rates of infertility, miscarriage, and trisomic conceptions. Despite the significance of the problem, strategies to sustain oocyte quality with age have remained elusive. Here we report that adult female mice maintained under 40% caloric restriction (CR) did not exhibit aging-related increases in oocyte aneuploidy, chromosomal misalignment on the metaphase plate, meiotic spindle abnormalities, or mitochondrial dysfunction (aggregation, impaired ATP production), all of which occurred in oocytes of age-matched ad libitum-fed controls. The effects of CR on oocyte quality in aging females were reproduced by deletion of the metabolic regulator, peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α). Thus, CR during adulthood or loss of PGC-1α function maintains female germline chromosomal stability and its proper segregation during meiosis, such that ovulated oocytes of aged female mice previously maintained on CR or lacking PGC-1α are comparable to those of young females during prime reproductive life.bioenergetics | fertility | germ cell | oogenesis | ovary

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“…In combination with vitamin C, vitamin E decreased the frequency of litters, litter size, total number of offspring born and survival of male pups to weaning. This effect was associated with lower number of corpora lutea in the left ovary, decreased percentage of viable fetuses, and higher number of fetal resorptions in the left uterine horn when compared to the control group 10 .…”

Section: Introductionmentioning

confidence: 84%

Sigara Dumanına Maruz Kalan Dişi Sıçanlarda Alfa-Tocopherol"ün Endometrial Uterin Vaskülarizasyonu, Rahim Serviks Oksidatif Stres ve Proliferasyon Üzerine Etkisi

Purba¹,

Aritonang²,

Nurdiana³

et al. 2014

Cukurova Medical Journal

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Purpose: This study aimed toknow whether administration of α-tocopherolable to increase uterine endometrial vascularization, inhibit uterine cervix oxidative stress, and augment cervical proliferation in female rats exposed to tobacco smoke. Material and Methods:Twenty five, 8 weeks old female Wistar rats (Rattusnorvegicus) were divided into 5 groups (n=5 each), including control group (non exposure group); the rats were exposed to tobacco smoke (CS); the rats were exposed to tobacco smoke and treated with α-tocopherol at dose of 100 mg/kg/day (CS + AT1); rats were exposed to tobacco smoke and treated with α-tocopherol at dose of 200mg/kg/day (CS + AT2); rats were exposed to tobacco smoke and treated with α-tocopherol at dose of 400 mg/kg/day (CS + AT3). The expression of endometrial VEGF was observed by immunohistochemical staining using anti-VEGF primary antibody and biotinylated universal secondary antibody. MDA levels were measured colorimetrically. The number of spiralis arterioles and proliferation of uterine cervix epithelial cells were counted histologically withhematoxilin eosin staining. This research has been approved by research ethics committee Faculty of Medicine University of Brawijaya, Malang, Indonesia (Number 477/EC/KEPK-S2-KB/10/2013). Results: Tobacco smoke significantly lowered VEGF level, the number of spiralis arterioles, anduterine cervix epithelial cells compared with control group (P < 0.05). Administration of α-tocopherol in different doses significantly increasethe VEGF level to reachs the level in the control group (P > 0.05). Second and third doses of α-tocopherolsignificantly increased the number of spiralis arterioles compared with CS group (P < 0.05), to reached the similar level in the control group (P > 0.05).Exposure to tobacco smoke in rats can increase uterine cervix MDA level significantly compared to the control (P< 0.05). The α-tocopherol in various doses cansignificantly reduce MDA level compared to the CS (P <0.05), but has not reached the levels of the control group. The number of epithelial cells in α-tocopherol-administered groupwas not significantly different compared with CS group (P > 0.05). Sonuç: α-tocopherol'ün uygulanması sigara dumanına maruz kalmış sıçanlarda endometrial uterinde vaskülarizasyonu arttırabilmektedir. Bunun yanı sıra, α-tocopherol uterin serviks oksidatif stresinİ hibe etmektedir, fakat uterin serviks epithelial hücrelerin çoğalmasını indüklememektedir. Conclusion

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Oral administration of pharmacological doses of Vitamins C and E reduces reproductive fitness and impairs the ovarian and uterine functions of female mice

Cited by 35 publications

References 39 publications

Changes of vitamins A, E, and C and lipid peroxidation status of breeding and pregnant sheep during dry seasons on medium-to-low quality forages

Changes of vitamins A, E, and C and lipid peroxidation status of breeding and pregnant sheep during dry seasons on medium-to-low quality forages

Prevention of maternal aging-associated oocyte aneuploidy and meiotic spindle defects in mice by dietary and genetic strategies

Sigara Dumanına Maruz Kalan Dişi Sıçanlarda Alfa-Tocopherol"ün Endometrial Uterin Vaskülarizasyonu, Rahim Serviks Oksidatif Stres ve Proliferasyon Üzerine Etkisi

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