Optofluidic fluorescence cell for the detection of low concentration toxic gases

Allouch, Alaa; Guglielmino, Maud; Serra, Christophe A.; Calvé, Stéphane Le

doi:10.1016/j.snb.2017.09.174

Cited by 6 publications

(2 citation statements)

References 19 publications

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“…In recent years, the liquid-core waveguide (LCW) has attracted substantial interests [19]- [22]. The LCW enables strong interaction between the excitation and the sample under analysis, thereby allowing high-sensitivity fluorescence detection with reduction in sample volume.…”

Section: Introductionmentioning

confidence: 99%

“…This unique advantage offers vast potential for the development of lowcost microsystems for fluorescence detection. In the last several years, optofluidic platforms based on the LCW have been investigated for different applications, such as for low concentration toxic gases detection [19], [23], real-time single molecule detection [24], flowing micro droplet based absorbance detection [18], and single-virus detection [17], [25].…”

Section: Introductionmentioning

confidence: 99%

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Fully Integrated Liquid-Core Waveguide Fluorescence Lifetime Detection Microsystem for DNA Biosensing

et al. 2019

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Time-resolved fluorescence is a widely adopted technique for DNA detection due to its high sensitivity and selectivity. However, due to stringent requirements on optics and electronics, instrumentation with time-resolved capability is bulky and expensive, prohibiting their use in portable and point-of-care applications. In this work, a fully-integrated DNA biosensor based on liquid-core waveguide (LCW) optics for fluorescence lifetime analysis is presented. The DNA biosensor encompasses all-custom bioassay, optics and electronics into a microsystem, delivering a near sample-to-answer level of integration. Lifetime, rather than intensity, is exploited as the analytical signal from the V-carbazole probe for the first time. Excitation propagation within the LCW is investigated both analytically and in simulations to achieve high excitation rejection and low temporal dispersion, enabling the proposed LCW-based system with much smaller instrumentation size to deliver comparable lifetime measurement accuracy to traditional systems. Detection of DNA down to 15 base pairs at a low detection limit of 1.38 nM demonstrates the high applicability of the proposed biosensor for compact, application-specific, and low-cost diagnostics devices.INDEX TERMS DNA biosensing, fluorescence lifetime detection, liquid-core waveguide, TCSPC, turn-on fluorescent probe.

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Section: Introductionmentioning

confidence: 99%