Optofluidic-based deformability cytometry: Characterizing erythrocyte elasticity and assessing storage quality

Mao, Yuxin; Li, Songlin; Liu, Rui; Zhang, Can; Tan, Xinyuan; Zhong, Zhensheng; Wang, Yi; Zhou, Jinhua

doi:10.1016/j.sna.2024.115182

Cited by 2 publications

(1 citation statement)

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“…The home-built optical tweezers device was described in previous studies [ 19 , 21 – 23 ]. Briefly, a 1064-nm fiber laser (Amonics Ltd. AFL-1064–37-R-CL, Hong Kong, China) was expanded by two lenses and focused by a water immersion objective (×60, NA 1.20, Olympus, Japan) in an inverted microscope (IX73, Olympus, Japan), generating an optical trap.…”

Section: Methodsmentioning

confidence: 99%

Time and power dependence of laser-induced photodamage on human sperm revealed by longitudinal rolling measurement using optical tweezers

He,

Wei,

Wang

et al. 2024

Biomed. Opt. Express

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Lasers are widely applied in assisted reproductive technologies, including sperm fixation, sperm selection and intracytoplasmic sperm injections, to reduce procedure time and improve consistency and reproducibility. However, quantitative studies on laser-induced photodamage of sperm are lacking. In this study, we demonstrated that, by using optical tweezers, the kinematic parameters of freely swimming sperm are correlated with the frequency as well as the percentage of pausing duration of longitudinal rolling of the same sperm head in the optical trap. Furthermore, by trapping individual sperm cells using 1064-nm optical tweezers, we quantitatively characterized the time-dependence of longitudinal rolling frequency and percentage of pausing duration of sperm under different laser powers. Our study revealed that, as trapping time and the laser power time increase, the longitudinal rolling frequency of the optically trapped sperm decreases with an increasing percentage of pausing duration, which characterizes the effect of laser power and duration on the photodamage of individual sperm cells. Our study provides experimental basis for the optimization of laser application in assisted reproductive technology, which may reduce the photodamage-induced biosafety risk in the future.

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Section: Methodsmentioning

confidence: 99%