Optimization of Ultrasonic-Microwave Assisted Extraction and Hepatoprotective Activities of Polysaccharides from Trametes orientalis

Zheng, Yi; Cui, Jue; Chen, Anhui; Zong, Zhi‐Min; Wei, Xian‐Yong

doi:10.3390/molecules24010147

Cited by 27 publications

(13 citation statements)

References 40 publications

(44 reference statements)

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“…Transaminases are rapidly released into blood stream when injuries or diseases affected the body tissues [ 34 ]. The results of the present study unveiled that the levels of AST, ALT, and ALP in the mouse model of acute alcoholic liver injury were significantly increased in serum, which is consistent with findings of previous reports [ 35 ]. This indicated that the mouse model of acute alcoholic liver injury was successfully established.…”

Section: Discussionsupporting

confidence: 93%

Chemical Components and Hepatoprotective Mechanism of Xwak Granule in Mice Treated with Acute Alcohol

Chen

Liu²,

Abdulla³

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Objective. To evaluate the hepatoprotective mechanism of Xwak granule (Xwak) in treatment of mice with alcoholic liver injury via activating ERK/NF-κB and Nrf/HO-1 signaling pathways. Methods. The chemical composition of Xwak was tested by liquid chromatography coupled with mass spectrometry (LC-MS). Herein, 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay and 2,2-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical tests were performed in vitro. The hepatoprotective effect of Xwak was assessed at different concentrations (1.5, 3, and 6 g/kg) in a mouse model of alcoholic liver injury. Results. Totally, 48 compounds, including 16 flavonoids, 8 tannins, 9 chlorogenic acids, and 15 other compounds, were identified from Xwak. Xwak showed to have a satisfactory antioxidant activity in vitro. In a group of Xwak-treated mice, the serum levels of alanine transaminase (ALT), aspartate transaminase (AST), and alkaline phosphatase (ALP) were decreased compared with a group of the mouse model of alcoholic liver injury. In addition, the levels of antioxidant enzymes, such as glutathione peroxidase (GSH-PX), total superoxide dismutase (T-SOD), and catalase (CAT), were noticeably increased and the levels of malondialdehyde (MDA), tumor necrosis factor-α (TNF-α), transforming growth factor-β (TGF-β), and interleukin-6 (IL-6) were markedly reduced in the liver of mice. The state of oxidative stress in the mouse model of alcoholic liver injury was improved after treatment with Xwak. The improvement of inflammation-mediated disruption may conducive to the Xwak activity in the control of liver injury. The signals of p-ERK1/2, p-NF-κB, COX-2, iNOS, CYP2E1, Nrf, and HO-1 were significantly induced in the liver of mice after treatment with Xwak. Conclusions. The abovementioned findings indicated that the hepatoprotective mechanism of Xwak could be achieved by activating ERK/NF-κB and Nrf/HO-1 signaling pathways to alleviate oxidative stress and inflammatory.

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Section: Discussionsupporting

confidence: 93%

Chemical Components and Hepatoprotective Mechanism of Xwak Granule in Mice Treated with Acute Alcohol

Chen

Liu²,

Abdulla³

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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“…It has been shown that the bioactivity of polysaccharides with 3D conformation is higher than those without a 3D structure. [26] Therefore, this result suggested that GFP-1-a and GFP-2-a are potentially bioactive polysaccharides.…”

Section: Conformational Resultsmentioning

confidence: 81%

“…As shown in Figure 1a, the best liquid-material ratio was 30 mL g -1 . The polysaccharide in G. frondosa could not be completely extracted when the liquid-material ratio was low, [26] but if the liquid-material ratio was too high the yield would not be increased but the process would be wasteful of energy and materials, and more acetone would also be consumed in subsequent treatment.…”

Section: Influence Of Raw Materials Extraction Process On Polysaccharide Yieldmentioning

confidence: 99%

Optimization of Extraction Technology, Structure, and Antioxidant Activity of Polysaccharide from Grifola frondosa

2021

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Using DMSO as solvent, ultrasonic-assisted extraction of polysaccharides from Grifola frondosa is studied. The effects of different extraction conditions on the yield of polysaccharides are investigated. The optimal extraction conditions are found by a Box-Behnken design. The results showed that the yield of Grifola frondosa polysaccharide can reach 6.94% under the following conditions: liquid-solid ratio of 33.8 mL g -1 , ultrasonic power of 104.6 W, extraction temperature of 35.6°C, and extraction time of 4.7 h. The structure of the polysaccharides is investigated by infrared spectroscopy, nuclear magnetic resonance, molecular weight, monosaccharide composition, and methylation. The purified polysaccharides are found to have good antioxidant activity in vitro.

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“…The yield of BS mucilage increased with increasing extraction time and an increasing liquid–solid ratio, but the yield then decreased as these two variables increased further. The response surface slope of the interaction between the extraction time and liquid–solid ratio was steep and the contour plot was elliptical, which suggested that the interaction between the two variables was extremely significant [29]. Figure 1E evaluates the effect of the shear speed and liquid–solid ratio on the yield of BS mucilage.…”

Section: Resultsmentioning

confidence: 99%

Optimizing the Extraction and Encapsulation of Mucilage from Brasenia Schreberi

Luo

Sun

et al. 2019

Polymers

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The mucilage from Brasenia schreberi (BS) exhibits various biological activities, including antialgal, antibacterial, soluble-fiber properties, and excellent lubricating behavior. Thus, the extraction and wide use of mucilage in the food industry are crucial. In this study, the high-speed shear-assisted extraction of mucilage from BS was optimized by using response surface methodology (RSM). The optimal extraction conditions were as follows: Extraction temperature of 82 °C, extraction time of 113 min, liquid–solid ratio of 47 mL/g, and shear speed of 10,000 rpm. Under these conditions, the actual yield of BS mucilage was 71.67%, which highly matched the yield (73.44%) predicted by the regression model. Then, the BS mucilage extract was powdered to prepare the capsule, and the excipients of the capsule were screened using a single-factor test to improve the disintegration property and flowability. The final capsule formulation, which consisted of: 39% BS mucilage powder (60 meshes); 50% microcrystalline cellulose (60 meshes) as the filler; both 10% sodium starch glycolate and PVPP XL-10 (3:1, 60 meshes) as the disintegrant; both 1% colloidal silicon dioxide and sodium stearyl fumarate (1:1, 100 meshes) as the glidant by weight; were used for preparing the weights of a 320 mg/grain of capsule with 154.7 ± 0.95 mg/g polysaccharide content. Overall, the optimized extraction process had a high extraction rate for BS mucilage and the capsule formulation was designed reasonably.

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Optimization of Ultrasonic-Microwave Assisted Extraction and Hepatoprotective Activities of Polysaccharides from Trametes orientalis

Cited by 27 publications

References 40 publications

Chemical Components and Hepatoprotective Mechanism of Xwak Granule in Mice Treated with Acute Alcohol

Chemical Components and Hepatoprotective Mechanism of Xwak Granule in Mice Treated with Acute Alcohol

Optimization of Extraction Technology, Structure, and Antioxidant Activity of Polysaccharide from Grifola frondosa

Optimizing the Extraction and Encapsulation of Mucilage from Brasenia Schreberi

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