Optimization of the l-tyrosine metabolic pathway in Saccharomyces cerevisiae by analyzing p-coumaric acid production

Li, Yuanzi; Mao, Jiwei; Song, Xiaofei; Wu, Yuzhen; Cai, Mingzhong; Wang, Hesuiyuan; Liu, Quanli; Zhang, Xiuming; Bai, Yanling; Xu, Haijin; Qiao, Mingqiang

doi:10.1007/s13205-020-02223-3

Cited by 6 publications

(1 citation statement)

References 33 publications

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“…Accordingly, knockout of these genes, such as ARO10, can stimulate the formation of p -coumaric acid (Figure S2B). Surprisingly, deletion of these genes had no effect on (2 S )-eriodictyol titer, possibly because excess p -coumaric acid could not be converted into (2 S )-eriodictyol . Thus, it is essential to optimize the (2 S )-naringenin synthetic pathway starting from l -tyrosine.…”

Section: Resultsmentioning

confidence: 99%

High-Level De Novo Production of (2S)-Eriodictyol in Yarrowia Lipolytica by Metabolic Pathway and NADPH Regeneration Engineering

Yue,

Liu,

Gao

et al. 2024

J. Agric. Food Chem.

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Metrics & MoreArticle Recommendations * sı Supporting Information ABSTRACT: (2S)-Eriodictyol, a polyphenolic flavonoid, has found widespread applications in health supplements and food additives. However, the limited availability of plant-derived (2S)-eriodictyol cannot meet the market demand. Microbial production of (2S)-eriodictyol faces challenges, including the low catalytic efficiency of flavone 3′-hydroxylase/cytochrome P450 reductase (F3′H/CPR), insufficient precursor supplementation, and inadequate NADPH regeneration. This study systematically engineered Yarrowia lipolytica for high-level (2S)-eriodictyol production. In doing this, the expression of F3′H/CPR was balanced, and the supply of precursors was enhanced by relieving feedback inhibition of the shikimate pathway, promoting fatty acid β-oxidation, and increasing the copy number of synthetic pathway genes. These strategies, combined with NADPH regeneration, achieved an (2S)eriodictyol titer of 423.6 mg/L. Finally, in fed-batch fermentation, a remarkable 6.8 g/L (2S)-eriodictyol was obtained, representing the highest de novo microbial titer reported to date and paving the way for industrial production.

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Section: Resultsmentioning

confidence: 99%