Optimization of starch- and chitosan-based bio-inks for 3D bioprinting of scaffolds for neural cell growth

Butler, Haley; Naseri, Emad; MacDonald, Debra S.; Tasker, R. Andrew; Ahmadi, Ali

doi:10.1016/j.mtla.2020.100737

Cited by 23 publications

(24 citation statements)

References 33 publications

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“…Preparation of Biomaterial Inks: NOCC was synthesized as described in a previous work. [34] Briefly, as shown in Figure 7, chitosan powder (>75% deacetylated) from shrimp shells (Sigma-Aldrich C3646), 10N sodium hydroxide, isopropanol, and deionized (DI) water were combined and stirred for 2.5 h at room temperature. A monochloroacetic acid and isopropanol solution was added dropwise to the chitosan solution for 0.5 h and was subsequently stirred (200 rpm) and heated (50 °C) for 4 h. The solution was filtered over a 20 μm mesh, washed with 90% ethyl alcohol, and dried overnight.…”

Section: Methodsmentioning

confidence: 99%

Development of N,O‐Carboxymethyl Chitosan‐Starch Biomaterial Inks for 3D Printed Wound Dressing Applications

Naseri

Cartmell

Saab

et al. 2021

Macromolecular Bioscience

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In this paper, a novel hybrid biomaterial ink consisting of two water-soluble polymers is investigated: starch and N,O-carboxymethyl chitosan (NOCC). The biomaterial ink is used to fabricate controlled release biodegradable wound dressing scaffolds via a novel low-temperature solvent (organic)-free 3D printing technique. NOCC is a variant of chitosan with a high degradation rate that can lead to an immediate release of the drugs, and starch, on the other hand, is used to alter degradation and drug release characteristics of the biomaterial. Mupirocin, a topical anti-infective, is incorporated into the biomaterial inks. Different biomaterial inks in terms of NOCC to starch ratio are prepared and characterized. Printability and rheology of the samples are investigated, and the release of mupirocin over time is quantified. The efficacy of the developed 3D printed wound dressings against Staphylococcus aureus is examined through disk diffusion assays. Increasing NOCC accelerated the release of the drug from the scaffold and led to larger zones of inhibition in the early hours of the in vitro tests; this phenomenon is correlated to the enhanced hydrophilicity of NOCC-dominated scaffolds. The drug release and the zone of inhibition are controlled by altering starch to NOCC ratio in the biomaterial ink.

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Section: Methodsmentioning

confidence: 99%

Development of N,O‐Carboxymethyl Chitosan‐Starch Biomaterial Inks for 3D Printed Wound Dressing Applications

Naseri

Cartmell

Saab

et al. 2021

Macromolecular Bioscience

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“…The variety of drugs, active substances, and forms constantly require the search for new solutions to provide the delivery of the active substance. Among the various delivery systems, nanoparticles [266,270,271,273,274] and microparticles [269,272] are the most recognized and popular forms of carriers.…”

Section: Chitosan Microparticles and Nanoparticles As Drug Carriersmentioning

confidence: 99%

“…The recently described systems consisting of chitosan as main or additive material are shown in Table 1. In some cases, chitosan particles can increase Drug Loading Efficiency (DLE) [266,[269][270][271]273,274] and Drug Encapsulation Efficiency (DEF) [266,269,274], which is a crucial parameter in drug delivery systems.…”

Section: Chitosan Microparticles and Nanoparticles As Drug Carriersmentioning

confidence: 99%

Chitosan as an Underrated Polymer in Modern Tissue Engineering

Kołodziejska¹,

Jankowska²,

Klak³

et al. 2021

Nanomaterials

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Chitosan is one of the most well-known and characterized materials applied in tissue engineering. Due to its unique chemical, biological and physical properties chitosan is frequently used as the main component in a variety of biomaterials such as membranes, scaffolds, drug carriers, hydrogels and, lastly, as a component of bio-ink dedicated to medical applications. Chitosan’s chemical structure and presence of active chemical groups allow for modification for tailoring material to meet specific requirements according to intended use such as adequate endurance, mechanical properties or biodegradability time. Chitosan can be blended with natural (gelatin, hyaluronic acid, collagen, silk, alginate, agarose, starch, cellulose, carbon nanotubes, natural rubber latex, κ-carrageenan) and synthetic (PVA, PEO, PVP, PNIPPAm PCL, PLA, PLLA, PAA) polymers as well as with other promising materials such as aloe vera, silica, MMt and many more. Chitosan has several derivates: carboxymethylated, acylated, quaternary ammonium, thiolated, and grafted chitosan. Its versatility and comprehensiveness are confirming by further chitosan utilization as a leading constituent of innovative bio-inks applied for tissue engineering. This review examines all the aspects described above, as well as is focusing on a novel application of chitosan and its modifications, including the 3D bioprinting technique which shows great potential among other techniques applied to biomaterials fabrication.

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“…In a recent example, Zhange et al developed bioinks with the same biological activity as original cartilage extracellular matrices, which could be used to construct scaffolds for cartilage tissue engineering [97]. In another example, Butler et al developed a bioink from a blend of chitosan and starch [98]. This bioink showed great printability and biocompatibility with great promise for neural tissue engineering applications.…”

Section: Biomaterialsmentioning

confidence: 99%

Recent Trends and Innovation in Additive Manufacturing of Soft Functional Materials

et al. 2021

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The growing demand for wearable devices, soft robotics, and tissue engineering in recent years has led to an increased effort in the field of soft materials. With the advent of personalized devices, the one-shape-fits-all manufacturing methods may soon no longer be the standard for the rapidly increasing market of soft devices. Recent findings have pushed technology and materials in the area of additive manufacturing (AM) as an alternative fabrication method for soft functional devices, taking geometrical designs and functionality to greater heights. For this reason, this review aims to highlights recent development and advances in AM processable soft materials with self-healing, shape memory, electronic, chromic or any combination of these functional properties. Furthermore, the influence of AM on the mechanical and physical properties on the functionality of these materials is expanded upon. Additionally, advances in soft devices in the fields of soft robotics, biomaterials, sensors, energy harvesters, and optoelectronics are discussed. Lastly, current challenges in AM for soft functional materials and future trends are discussed.

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Optimization of starch- and chitosan-based bio-inks for 3D bioprinting of scaffolds for neural cell growth

Cited by 23 publications

References 33 publications

Development of N,O‐Carboxymethyl Chitosan‐Starch Biomaterial Inks for 3D Printed Wound Dressing Applications

Development of N,O‐Carboxymethyl Chitosan‐Starch Biomaterial Inks for 3D Printed Wound Dressing Applications

Chitosan as an Underrated Polymer in Modern Tissue Engineering

Recent Trends and Innovation in Additive Manufacturing of Soft Functional Materials

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