Background:
Alkaline protease essential enzymes that have several applications in our industry.
Objective:
The aim was optimization of nutritional parameters by one-factor-at-a-time (OFAT) method in solid-state fermentation.
Method:
Production of protease employing our laboratory new isolate, Alternaria alternata TUSG1 (strain accession number- MF401426) under solid-state fermentation was optimized. The nutritional factors was investigated and only one agricultural residue (cauliflower leaves) with different particle size was checked.
Results:
Highest enzyme production was obtained with medium particle size of cauliflower leaves (610 U/gds) followed by coarse waste (603U/gds) and fine waste (596 U/gds) using 106 spores/ml as inoculum at 30° C for 7 days. The organism utilized carbon sources 0.5 % (w/w) dextrose, fructose, maltose, sucrose, lactose and starch. Among them maltose was found to be the best carbon source. A variety of inorganic and organic media components were investigated for nitrogen sources 0.3 % (w/w) and skim milk turned out to the best.
Conclusion:
The maximum enzyme activity was obtained with 1% maltose, 0.5% skim milk and 0.05% MgSO4. With optimized media 1.53 fold increase in the protease production at agricultural residue cauliflower leaves was obtained.