Optimization of Mycelial Biomass Production in Submerged Culture Fermentation of <i>Pleurotus flabellatus</i> Using Response Surface Methodology

Abstract

The factors selected to optimize the productivity of Pleurotus flabellatus biomass in 250ml working volume Erlenmeyer flask were agitation rate, initial pH value and incubation temperature. The central composite design was applied to study the significant factors and the interactions between the chosen factors, if present. The Design Expert software generated 20 runs. The optimized conditions obtained were as follows: the agitation rate of 129.8 rpm, incubation temperature at 27.8˚C, and initial pH of 6.06. The optimized conditions tripled the productivity at the range of 980-1040 mg/litre/day compared to the initial rate productivity at 310 mg/litre/day. From the quadratic equation,the agitation rate, temperature and the interaction between agitation rate and temperature were found to be significant (p < 0.05). At optimum conditions, the experimental data supported the theoretical estimate.

Biochemical Evaluation of Agaricus and Pleurotus Strains in Batch Cultures for Production Optimization of Valuable Metabolites

“…Thus, optimization for future continuous cultures will need large-scale experimental designs that become cumbersome and costly. As a first step for optimizing future continuous production, batch-culture results were further statistically analyzed using response 3D surfaces and contour graphics to extrapolate potential culture parameters [ 81 ]. Results of this analysis are presented in Figure 7 , where glucans and glucanases are plotted against glucose consumption and the corresponding biomass-production rate in order to determine optimal dilution and flow rates in future continuous cultures.…”

Studies on Optimization of Culture Conditions and Medium Components for the Production of Mycelial Biomass of Auricularia delicata under Submerged Fermentation

“…7H 2 0 is due to the presence of P and Mg minerals. K 2 HPO 4 promotes mycelia growth through its buffering capacity and release of phosphates meanwhile, MgSO 4 releases Mg ion, an essential cofactor for some enzymes involved in the biosynthesis of bioactive metabolites [56,[60][61][62]. The medium containing CaSO 4 showed a low mycelia growth due to the capacity of Ca decelerate mycelial growth.…”