Optimization of multi-enzyme cascade process for the biosynthesis of benzylamine
Jinli Wang,
Runan Dong,
Jingxin Yin
et al.
Abstract:Benzylamine is a valuable intermediate in the synthesis of organic compounds such as curing agents and antifungal drugs. To improve the efficiency of benzylamine biosynthesis, we identified the enzymes involved in the multi-enzyme cascade, regulated the expression strength by using RBS engineering in Escherichia coli, and established a regeneration-recycling system for alanine. This is a co-substrate, coupled to cascade reactions, which resulted in E. coli RARE-TP and can synthesize benzylamine using phenylala… Show more
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