An inverted pyramidal
metasurface was designed, fabricated, and
studied at the nanoscale level for the development of a label-free
pathogen detection on a chip platform that merges nanotechnology and
surface-enhanced Raman scattering (SERS). Based on the integration
and synergy of these ingredients, a virus immunoassay was proposed
as a relevant proof of concept for very sensitive detection of hepatitis
A virus, for the first time to our best knowledge, in a very small
volume (2 μL), without complex signal amplification, allowing
to detect a minimal virus concentration of 13 pg/mL. The proposed
work aims to develop a high-flux and high-accuracy surface-enhanced
Raman spectroscopy (SERS) nanobiosensor for the detection of pathogens
to provide an effective method for early and easy water monitoring,
which can be fast and convenient.