Optimization of Luteolin-Loaded Transfersome Using Response Surface Methodology

Abstract

Objective: This research was carried out to optimize luteolin-loaded transfersome formula with independent variables such as lipid-surfactant (total lipid) concentration and luteolin concentration.Methods: Luteolin-loaded transfersome was optimized by response surface methodology based on four parameters, namely, particle size (Z-average), polydispersity index, zeta potential, and entrapment efficiency. The transfersome formula was prepared using central composite design, and the selected independent variables were the total lipid (mixture of phospholipid and Tween 80) and luteolin concentrations. 14 formulas of luteolinloaded transfersome were prepared by thin film hydration, followed by the sonication method. Results:The total lipid and luteolin concentration significantly affected the entrapment efficiency only. The other parameters were not affected by a change in these variables. The optimum formula of 4.88% total lipid and 0.5% luteolin with desirability value of 0.609 conformed with the prediction parameters. Vesicle imaging using transmission electron microscopy revealed spherical particles and the occurrence of particle aggregation. The optimum formula of luteolin-loaded transfersome possessed the following characteristics: Particle size of 286.03±8.46 nm, polydispersity index of 0.480±0.013, zeta potential of -18.67±0.379 mV, and entrapment efficiency of 94.97±0.28 %. However, these values did not correspond to the predicted values and were confirmed by the low adjusted and predicted R-squared values. Conclusion:This method can be applied to optimize the entrapment efficiency, and in the future, it can be used for further optimizing formula of transfersome by including more variables.

Preparation and in Vitro Evaluation of Soya Lecithin Based Nano Transfersomal Dispersion for Loxoprofen Sodium

“…Most of the formulas showed good entrapment efficiency indicating that the thin film method used for their preparation is efficient and reliable. The results agreed with reported data where this method was reported to be applicable for different lipid based vesicles [27,28] . The effect of different variables on entrapment efficiency was further studied.…”

“…Effect of (lipid /surfactant) total percentage on entrapment efficiency: When the formula (SF17) was re-prepared using 3% total lipid /surfactant dispersion, the entrapment efficiency was decreased significantly (p < 0.05) to 53% in comparison to that prepared using 6% total lipid /surfactant which was 89%. this indicates that as the total content of the transfersomes ( lipid and edge activators) increased the vesicles number was increased leading to an increase in entrapment efficiency [28,32] .…”

Evaluation of Ethosomal Epigallocatechin-3-Gallate Gel Formulations as an Antioxidant and Thermogenic Agent

“…The measurements were repeated. The polydispersity index was conducted to evaluate the particle distribution of ethosomes [17][18][19].…”

Placenta Extract-Loaded Novasome Significantly Improved Hair Growth in a Rat in Vivo Model

“…Vesicle composition and pH value have an effect on zeta potential [30]. Novasomes from all formulas showed negative ZP values ranging from-24.07 to-63.73 mV (table 3), which might be caused by the negative charge of the fatty acid.…”