Optimization of HPLC method for stability testing of bacitracin

Pavli, Viljem; Kmetec, Vojko

doi:10.1016/s0731-7085(00)00569-0

Cited by 30 publications

(27 citation statements)

References 7 publications

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“…F splits into FЈ and FЉ). 8,25) A pronounced splitting of peaks of oxidative degradation products H2, H3 and F, with the exception of H1, was observed on conventional reversed phase HPLC columns when acidic mobile phase with pH 2 was used (Fig. 4).…”

Section: Resultsmentioning

confidence: 97%

Pathways of Chemical Degradation of Polypeptide Antibiotic Bacitracin

Pavli

Kmetec

2006

Biological & Pharmaceutical Bulletin

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Section: Resultsmentioning

confidence: 97%

Pathways of Chemical Degradation of Polypeptide Antibiotic Bacitracin

Pavli

Kmetec

2006

Biological & Pharmaceutical Bulletin

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“…Bacitracin A was separated from its congeners by HPLC using a semipreparative Ultrasphere 5 ODS (C18) column (Hichrom), as described previously (41). Peak fractions were diluted fivefold in water, desalted on a Sep-Pak Vac 12 mL C18 cartridge (Waters), lyophilized, and redissolved in 10 mM Hepes, pH 7.5.…”

Section: Methodsmentioning

confidence: 99%

High-resolution crystal structure reveals molecular details of target recognition by bacitracin

Economou

Simon

Loll

2013

Proc. Natl. Acad. Sci. U.S.A.

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Bacitracin is a metalloantibiotic agent that is widely used as a medicine and feed additive. It interferes with bacterial cell-wall biosynthesis by binding undecaprenyl-pyrophosphate, a lipid carrier that serves as a critical intermediate in cell wall production. Despite bacitracin’s broad use, the molecular details of its target recognition have not been elucidated. Here we report a crystal structure for the ternary complex of bacitracin A, zinc, and a geranyl-pyrophosphate ligand at a resolution of 1.1 Å. The antibiotic forms a compact structure that completely envelopes the ligand’s pyrophosphate group, together with flanking zinc and sodium ions. The complex adopts a highly amphipathic conformation that offers clues to antibiotic function in the context of bacterial membranes. Bacitracin’s efficient sequestration of its target represents a previously unseen mode for the recognition of lipid pyrophosphates, and suggests new directions for the design of next-generation antimicrobial agents.

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“…Microbiological method for quantitative assay of Bc is still official [4,6]; however, the HPLC method for quality control of Bc and for the evaluation of component F was currently bit shorter [13]. The aim of this study was to develop an effective, stability-indicating and faster HPLC separation of Bc for daily routine quality control.…”

Section: Introductionmentioning

confidence: 99%