Optimization of Growth Medium and Enzyme Assay Conditions for Crude Cellulases Produced by a Novel Thermophilic and Cellulolytic Bacterium, Anoxybacillus sp. 527

Liang, Yanna; Feng, Zisong; Yesuf, Jemil; Blackburn, James W.

doi:10.1007/s12010-009-8677-x

Cited by 66 publications

(42 citation statements)

References 47 publications

(42 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Different sets of enzymes are induced by different substrates (Liang et al 2010). Similar results was observed for a thermophilic Brevibacillus JXL as different optimum temperatures were observed in crude cellulase induced by glucose and by cellobiose (Liang et al 2009(Liang et al , 2010a. Recently, cellulase from a thermophilic B. vallismortis RG-07 recorded optimum activity at 65 °C, and 105 °C (Gaur and Tiwari 2015).…”

Section: Characterization Of Cellulase Enzyme Effect Of Ph and Tempersupporting

confidence: 77%

Enhanced Cellulase Production by a Novel Thermophilic Bacillus licheniformis 2D55: Characterization and Application in Lignocellulosic Saccharification

Kazeem¹,

Shah²,

Baharuddin³

et al. 2016

BioResources

View full text Add to dashboard Cite

Effects of nutritional and physicochemical factors were investigated for cellulase production by the newly isolated thermophilic strain Bacillus licheniformis 2D55 (Accession No. KT799651). The optimum cellulase production in shake flask fermentation was attained at 60 °C, pH 3.5, 180 rpm, and in a medium containing untreated sugarcane bagasse and pretreated rice husk at 7% (w/v), urea, 1 g/L, peptone, 11.0 g/L, Mg(SO4)2, 0.40 g/L, CaCl2, 0.03 g/L, Tween 80, 0.2% (w/v), and 3% inoculum. The highest carboxymethyl cellulase (CMCase), filtre paperase (FPase), and β-glucosidase produced under the optimized conditions were 29.4 U/mL, 12.9 U/mL, and 0.06 U/mL, respectively, after 18 h of fermentation. Optimization of the parameters increased the CMCase, FPase, and β-glucosidase activities by 77.4-fold, 44.5-fold, and 10-fold, respectively. The crude enzyme was highly active and stable over broad temperature (50 to 80 °C) and pH (3.5 to 10.0) ranges with optimum temperature at 65 °C and 80 ºC for CMCase and FPase, respectively. The optimum pH for CMCase and FPase was 7.5 and 6.0, respectively. Saccharification of sugar cane bagasse and rice husk by crude cellulase resulted in perspective yields of 0.348 and 0.301 g g -1 dry substrate of reducing sugars. These results suggest prospects of thermostable cellulase from B. licheniformis 2D55 in application for bio-sugar production and other industrial bioprocess applications involving high temperatures.

show abstract

Section: Characterization Of Cellulase Enzyme Effect Of Ph and Tempersupporting

confidence: 77%

Enhanced Cellulase Production by a Novel Thermophilic Bacillus licheniformis 2D55: Characterization and Application in Lignocellulosic Saccharification

Kazeem¹,

Shah²,

Baharuddin³

et al. 2016

BioResources

View full text Add to dashboard Cite

show abstract

“…Among four isolates, NAST PD13 showed highest cellulase (0.31 IU/ml), xylanase (3.02 IU/ml), and amylase (5.15 IU/ml) activity. Among Anoxybacillus sp., only A. flavithermus, and Anoxybacillus sp., 527 are reported as cellulose degrader [33]. However, in the present result, A. salvatliensis (NAST BR26) and A. kamchatkensis (NAST PD13) also degraded cellulose.…”

Section: Production Of Enzymes and Assessment Of Their Activitycontrasting

confidence: 60%

“…The temperature of hot springs was below 60°C except at Bhurung and Paudwar hot springs which show that any microbes do not necessarily have the same optimum temperature as their natural habitats [40]. [33], therefore, there was great probability of isolating mostly thermophilic bacilli from the hot springs. The use of other enrichment media may likely to recover different and some novel microbes [37].…”

Section: Discussionmentioning

confidence: 99%

Morphological, enzymatic screening, and phylogenetic analysis of thermophilic bacilli isolated from five hot springs of Myagdi, Nepal

2018

J App Biol Biotech

View full text Add to dashboard Cite

The present study was conducted to identify and characterize the thermophilic bacteria isolated from five hot springs, namely, Sinkosh, Singha, Bhurung, Ratopani, and Paudwar located in Myagdi district, Nepal, using phenotypic and genotypic methods. The hot spring has temperature 42-62°C and pH 6.5-6.8. Isolation of thermophiles was done using simple enriched nutrient broth media at 60°C. Selected strains were screened for thermostable enzymes; cellulase, hemicellulase, amylase, protease, gelatinase, and lipase using substrates carboxymethylcellulose, xylan, soluble starch, casein, gelatin, and Tween (20, 40, 60, or 80), respectively. The bacteria were grouped into 16 groups based on morphological and biochemical characteristics. 16S rRNA sequence analysis of 16 isolates and phylogenetic analysis showed a cluster of five distinct taxonomic groups. The groups were identified as genus Anoxybacillus, Aeribacillus, Brevibacillus, Bacillus, and Geobacillus, based on ≥95% similarity with reference strains. This is the first study that reports Anoxybacillus sp., Brevibacillus sp., and Aeribacillus sp. from the hot springs of Nepal.

show abstract

“…In another study reported by Nandimath et al [32], cellulase was found to be produced effectively within 60 min and at pH 5. Liang et al [56] further noted that growth medium pH strongly influences many enzymatic reactions by affecting the transport of a number of chemical products and enzymes across the cell membrane. For example, for the production of cellulase by Bacillus subtilis and Bacillus circulans, initial pH of growth medium in the range of 7-7.5 was found to be optimum [6].…”

Section: Discussionmentioning

confidence: 99%

Optimization of Cellulase and Xylanase Production by Micrococcus Species under Submerged Fermentation

et al. 2016

View full text Add to dashboard Cite

This paper reports on the optimization of culture conditions for cellulase and xylanase production by bacterial isolate from lignocellulosic biomass. The bacterial isolate was screened for cellulase and xylanase production on carboxyl methyl cellulose (CMC) and birch wood xylan as substrates, respectively. One bacterial isolate showing the highest halo zone diameter (isolate PLY1) was selected for detailed studies. The analysis of the 16S ribosomal ribonucleic acid (rRNA) gene nucleotide sequence of PLY1 revealed it to have 98% similarity to Micrococcus luteus strain Fse9 and the sequence was deposited in the GenBank as Micrococcus luteus strain SAMRC-UFH3 with accession number KU171371. Cellulase production was achieved in the presence of CMC (1% w/v) under an incubation temperature of 25 • C (198 U/mL), pH 5 (173 U/mL), agitation speed 50 rpm (173 U/mL) and incubation period of 96 h (102 U/mL). Xylanase was produced maximally when birch wood xylan (1% w/v) was used as the substrate at 25 • C (1007 U/mL), pH 10 (2487 U/mL), 200 rpm (1814 U/mL), and under an incubation period of 84 h (1296 U/mL). Our findings showed that Micrococcus sp. SAMRC-UFH3 appears to be a potentially important candidate for lignocellulosic waste degradation and other relevant industrial applications.

show abstract

Optimization of Growth Medium and Enzyme Assay Conditions for Crude Cellulases Produced by a Novel Thermophilic and Cellulolytic Bacterium, Anoxybacillus sp. 527

Cited by 66 publications

References 47 publications

Enhanced Cellulase Production by a Novel Thermophilic Bacillus licheniformis 2D55: Characterization and Application in Lignocellulosic Saccharification

Enhanced Cellulase Production by a Novel Thermophilic Bacillus licheniformis 2D55: Characterization and Application in Lignocellulosic Saccharification

Morphological, enzymatic screening, and phylogenetic analysis of thermophilic bacilli isolated from five hot springs of Myagdi, Nepal

Optimization of Cellulase and Xylanase Production by Micrococcus Species under Submerged Fermentation

Contact Info

Product

Resources

About