Optimization of fermentation parameters for high-activity inulinase production and purification from Rhizopus oryzae by Plackett–Burman and Box–Behnken

Yazici, Sercan Özbek; Şahin, Selmihan; Bıyık, Halil; Geroğlu, Yusuf; Özmen, İsmail

doi:10.1007/s13197-020-04591-3

Cited by 14 publications

(5 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The p -value for the response surface quadratic polynomial model of β-damascenone production was 0.0051, which indicated that the model was significant. Additionally, “the lack of fit” is a valid assessment test used to evaluate the reliability of a model, and a non-significant lack of fit is desirable ( Yazici et al, 2021 ). In this model, the lack of fit ( p = 0.1779) was not significant for the pure error, indicating that the model was fit.…”

Section: Resultsmentioning

confidence: 99%

Screening of β -damascenone-producing strains in light-flavor Baijiu and its production optimization via response surface methodology

Tang¹,

Lin²,

Jiang³

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

As a C13-norisoprenoid aroma substance, β-damascenone is a highly important aromatic compound and an active constituent. The purpose of this study was to investigate the change law of β-damascenone during the light-flavor Baijiu brewing process, and screen the indigenous microbial strains that produce this compound and optimize fermentation parameters for improving β-damascenone production using a statistical approach. In this project, Wickerhamomyces anomalus YWB-1 exhibited the highest producing activity of β-damascenone. Fermentation conditions were optimized for β-damascenone production using a one-factor-at-a-time (OFAT) approach. A Plackett-Burman design was subsequently adopted to assess the effects of initial pH, incubation temperature, inoculum size, fermentation period, and original Brix degree. Analysis of variance (ANOVA) showed that the correlation coefficient (R2) of the executive model was 0.9795, and this value was significant (p < 0.05). Three significant variables were optimized at three different coded levels using a Box-Behnken design (BBD) of response surface methodology (RSM). Here, 7.25 μg/L β-damascenone was obtained under the following optimum conditions: initial pH of 3.31, original Brix degree of 10.53%, and fermentation period of 52.13 h. The yield was increased 3.02-fold compared with that obtained under unoptimized conditions. This information is conducive to the control of flavor production by regulating variable parameters in Baijiu fermentation.

show abstract

Section: Resultsmentioning

confidence: 99%

Screening of β -damascenone-producing strains in light-flavor Baijiu and its production optimization via response surface methodology

Tang¹,

Lin²,

Jiang³

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Plants secrete far lower amounts of inulinase than microorganisms (Yuan et al, 2012 ; Singh et al, 2020 ). For instance, optimization of the parameters for inulinase production during Rhizopus oryzae -mediated fermentation using a statistical approach, resulted in maximum inulinase activity and specific activity of 348.36 EU/mL and 3621.78 EU/mg, respectively (Yazici et al, 2020 ).…”

Section: Resultsmentioning

confidence: 99%

Inactivation of Inulinase and Marination of High-Quality Jerusalem Artichoke (Helianthus tuberosus L.) Pickles With Screened Dominant Strains

Zhang

Liu

et al. 2021

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Freshly harvested Jerusalem artichoke tubers contain inulinase, an enzyme that requires inactivation, because of its ability to hydrolysis inulin into fructose, which can be consumed by microorganism during marination. As the traditional pickling process takes 6 months, and involves the addition of a large amount of salt (18–20%), this production strategy is uneconomical and increases the nitrite intake. Additionally, miscellaneous bacteria produced during pickling affect the product taste. In this study, the enzyme inactivation effects of NaCl, NaHCO3, and ultrasound were evaluated. NaHCO3 treatment results in the highest degree of enzyme inactivation; however, the quality and flavor of the obtained Jerusalem artichoke pickles were not ideal. The Jerusalem artichoke pickles in which the enzymes were inactivated using a combination of NaCl and ultrasound exhibited better flavor than those exposed to NaHCO3; further, this combination reduced the inulinase activity of the Jerusalem artichokes to 2.50 U/mL, and maintained the inulin content at 61.22%. The strains LS3 and YS2, identified as Enterococcus faecalis and the salt-tolerant yeast Meyerozyma guilliermondii, respectively, were the dominant microorganisms isolated from the pickle juice. Jerusalem artichokes with inactivated inulinase were pickled with microbial powder, separated, purified, and dried to remove the natural Jerusalem artichoke sauce. This process shortened the fermentation cycle and improved product quality.

show abstract

“…The optimization of the conditions for obtaining sugar mixtures was carried out by a two-stage method with the consistent use of Plackett-Burman and Box-Behnken factorial designs (Infanzón-Rodríguez et al, 2020;Ozbek et al, 2021;Devesa-Rey et al, 2023). To establish the significant parameters of WS hydrolysis, an experimental matrix was compiled according to the Plackett-Burman method, where seven influencing factors and two levels (minimum and maximum) of their values were used (Table 1).…”

Section: Methodsmentioning

confidence: 99%

Application of natural fungi in bioconversion of lignocellulosic waste to second-generation ethanol

Fomina,

Yurieva,

Pavlychenko

et al. 2024

Biosys. divers.

View full text Add to dashboard Cite

The second generation (2G) or cellulosic ethanol can help with diversification of the use of fossil energy sources. However, as bioconversion of plant waste into 2G bioethanol requires expensive additional steps of pre-treatment/hydrolysis of lignocellulosic materials, and this technology has not yet reached the technological readiness level which would allow it to be scaled-up, this process needs more interdisciplinary and comprehensive studies. This work was aimed at experimental study of a full cycle of successive processes of pre-treatment/saccharification using cellulolytic enzymes of filamentous fungi and fermentation of obtained syrups by xylose-fermenting yeast, using selected natural microorganisms for the fungal-based bioconversion of lignocellulosic agricultural waste to 2G ethanol. Using the Plackett-Burman and Box-Behnken methods of mathematical statistics, the optimal conditions for pre-treatment and enzymatic hydrolysis of wheat straw by a hemi- and cellulolytic multi-enzyme complex of the selected fungal strain Talaromyces funiculosus UCM F-16795 were established: microwave-assisted alkali pre-treatment with sodium hydroxide (NaOH) solutions (concentration range 4.6–4.8%), and saccharification conditions of medium pH 4, temperature 40 °С, hydrolysis duration 18 hours, and dilution of culture liquid with a buffer solution 1:1. The total energy of microwave irradiation 1.2 kJ and the ratio of substrate/enzyme solution 100 mg/1 mL were used. Under optimized conditions, wheat straw hydrolysates contained 5.0–7.5 g/L of reducing sugars, which, according to HPLC assessment, contained 0.7–1.0 g/L of glucose, 2.2–2.9 g/L of xylose and 0.7–0.8 g/L cellobiose. We used the selected strain of xylose-fermenting yeast in fermentation of mixtures of the most important monosaccharides in hydrolysates, xylose and glucose, in the concentration range relevant for syrups obtained by us during the optimized saccharification of lignocellulosic substrates with T. funiculosus enzymes. Based on sequencing and phylogenetic analysis, strain UCM Y-2810 was confirmed as Scheffersomyces stipitis; its nucleotide sequences of ITS region and 28S gene rDNA were deposited in GenBank under the accession numbers OP931914 and OP931915, respectively. The ethanologenic process for S. stipitis UCM Y-2810 was studied according to Box-Behnken design, assessing ethanol concentration by gas chromatography-mass spectrometry. Yeast fermentation under static microaerophilic conditions showed a 1.5 times higher rate of bioethanol production and 1.7 times greater efficiency of ethanologenesis per yeast biomass than for submerged cultivation. Optimization of the process of ethanologenesis resulted in the maximum rate of fermentation mixture of sugars, being 11.30 ± 0.36 g/L of ethanol, with optimal values of factors: 30 g/L of xylose, 5.5 g/L of glucose and cultivation for 5.5 days. It was revealed that the tested glucose concentrations did not significantly affect the process of xylose-fermentation by yeast, and non-competitive inhibition of xylose transport by glucose into yeast cells did not occur. This study demonstrated the potential of a full cycle bioconversion of lignocellulosic waste to 2G ethanol based on use of natural fungal strains and optimization of conditions for all steps.

show abstract

Optimization of fermentation parameters for high-activity inulinase production and purification from Rhizopus oryzae by Plackett–Burman and Box–Behnken

Cited by 14 publications

References 35 publications

Screening of β -damascenone-producing strains in light-flavor Baijiu and its production optimization via response surface methodology

Screening of β -damascenone-producing strains in light-flavor Baijiu and its production optimization via response surface methodology

Inactivation of Inulinase and Marination of High-Quality Jerusalem Artichoke (Helianthus tuberosus L.) Pickles With Screened Dominant Strains

Application of natural fungi in bioconversion of lignocellulosic waste to second-generation ethanol

Contact Info

Product

Resources

About