Optimization of conditions in on-line comprehensive two-dimensional reversed phase liquid chromatography. Experimental comparison with one-dimensional reversed phase liquid chromatography for the separation of peptides

Sarrut, Morgan; D’Attoma, Amélie; Heinisch, Sabine

doi:10.1016/j.chroma.2015.08.052

Cited by 66 publications

(54 citation statements)

References 42 publications

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“…Since the majority of LC × LC methods utilize gradient elution in the 2 D, it is not surprising that the main factor governing 2 t A (and indirectly, 1 t A ) is the 2 D gradient. Sarrut and co‐workers pointed out that the 2 D column equilibration and dwell volume play a significant role in the optimization of the total analysis time .…”

Section: Optimizationmentioning

confidence: 99%

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Optimizing separations in online comprehensive two‐dimensional liquid chromatography

Pirok

Gargano

Schoenmakers

2017

J of Separation Science

198

139

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Online comprehensive two‐dimensional liquid chromatography has become an attractive option for the analysis of complex nonvolatile samples found in various fields (e.g. environmental studies, food, life, and polymer sciences). Two‐dimensional liquid chromatography complements the highly popular hyphenated systems that combine liquid chromatography with mass spectrometry. Two‐dimensional liquid chromatography is also applied to the analysis of samples that are not compatible with mass spectrometry (e.g. high‐molecular‐weight polymers), providing important information on the distribution of the sample components along chemical dimensions (molecular weight, charge, lipophilicity, stereochemistry, etc.). Also, in comparison with conventional one‐dimensional liquid chromatography, two‐dimensional liquid chromatography provides a greater separation power (peak capacity). Because of the additional selectivity and higher peak capacity, the combination of two‐dimensional liquid chromatography with mass spectrometry allows for simpler mixtures of compounds to be introduced in the ion source at any given time, improving quantitative analysis by reducing matrix effects. In this review, we summarize the rationale and principles of two‐dimensional liquid chromatography experiments, describe advantages and disadvantages of combining different selectivities and discuss strategies to improve the quality of two‐dimensional liquid chromatography separations.

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Section: Optimizationmentioning

confidence: 99%

“…Sarrut and co-workers pointed out that the 2 D column equilibration and dwell F I G U R E 1 4 Schematic representation of an algorithmic optimization procedure. Reproduced from [187] with permission volume play a significant role in the optimization of the total analysis time [35].…”

Section: Other Quality Descriptorsmentioning

confidence: 99%

Optimizing separations in online comprehensive two‐dimensional liquid chromatography

Pirok

Gargano

Schoenmakers

2017

J of Separation Science

198

139

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“…Especially, the development of a two-dimensional chromatographic method can be a cumbersome and challenging task [182,183]. The sheer number of variables that must be taken into account [184] render a "trial and error" optimization impractical and time-consuming. Chemometrics tools may aid in almost all of the steps outlined in (Figure 15) [185].…”

Section: Introductionmentioning

confidence: 99%

Recent applications of chemometrics in one‐ and two‐dimensional chromatography

Bos

Knol

Molenaar

et al. 2020

J of Separation Science

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The proliferation of increasingly more sophisticated analytical separation systems, often incorporating increasingly more powerful detection techniques, such as highresolution mass spectrometry, causes an urgent need for highly efficient dataanalysis and optimization strategies. This is especially true for comprehensive twodimensional chromatography applied to the separation of very complex samples. In this contribution, the requirement for chemometric tools is explained and the latest developments in approaches for (pre-)processing and analyzing data arising from oneand two-dimensional chromatography systems are reviewed. The final part of this review focuses on the application of chemometrics for method development and optimization.

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“…With a few exceptions (Gargano et al, 2016;Sarrut et al, 2015;Schure, 1999;Vivó-Truyols et al, 2010), detection sensitivity (i.e., detection limits) has typically been overlooked as an important performance attribute. However, with the adoption of 2D-LC by more application-driven users, detection sensitivity is being identified as an increasingly important issue.…”

Section: Optimizing For Detection Sensitivitymentioning

confidence: 99%

“…Nevertheless, there are several other attributes of RP separations that make the RP Â RP combination very attractive, including high efficiency, miscibility of mobile phases used in the two separations, compatibility with mass spectrometric (MS) detection, and compatibility with large biomolecules (e.g., proteins). This has led us and others to focus on the use of RP Â RP separations to address a variety of separation problems ranging from the analysis of biological extracts to peptide separations (Sarrut et al, 2015;Vanhoenacker et al, 2015) and analysis of environmental contaminants . Readers interested in practical details associated with these types of separations are referred to the review of Li and Schmitz that is focused on this topic (Li et al, 2014b).…”

Section: Figure 78mentioning

confidence: 99%