Optimization of Alkaline   Α-Amylase Production by Thermophilic Bacillus Subtilis

Al-Johani, Nuha bakheet; Al‐Seeni, Madeha N.; Ahmed, Youssri

doi:10.21010/ajtcam.v14i1.31

Cited by 19 publications

(13 citation statements)

References 19 publications

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“…Then, the phylogenetic tree for these different species of Bacillus indicated that the strain was closely related to B. subtilis strain JCM 1465. This result is in agreement with a study that reported the optimization of amylase production by thermophilic B.subtilis (Al-Johani et al 2017). Additionally, Vijayalakshmi et al (2013) reported that Bacillus subtilis produces a more stable protease enzyme compared with B. licheniformis.…”

Section: Discussionsupporting

confidence: 91%

“…Hence, the formation of colorless hydrolytic zone around the bacteria is an indication of the carbohydrase activities. Then, the clear zone around the colonies in starch and inulin medium is an indication that the starch and inulin have been hydrolyzed by the amylase and inulinase, whereas the dark blue zone is formed through the reaction between carbohydrate and iodine (Al-Johani et al 2017). Furthermore, several studies have been conducted regarding bacteria inulinase activities.…”

Section: Discussionmentioning

confidence: 99%

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Isolation and molecular identification of carbohydrase and protease producing Bacillus subtilis JCM 1465 isolated from Penen Hot Springs in North Sumatra, Indonesia

Fachrial¹,

Anggraini²,

Harmileni³

et al. 2019

Biodiversitas

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Abstract. Fachrial E, Anggraini S, Harmileni, Nugroho TT, Saryono. 2019. Isolation and molecular identification of carbohydrase and protease producing Bacillus subtilis JCM 1465 isolated from Penen Hot Springs in North Sumatra, Indonesia. Biodiversitas 20: 3493-3498. The application of enzymes industrially is increasing every year and thermophilic microbes are a promising source of these enzymes for industrial use due to their temperature stability. The aim of this study, therefore, was to isolate, characterize and identify the enzyme producer-thermophilic bacteria from the Penen Hot Spring in Deli Serdang, North Sumatra, Indonesia. In the experimental setup, carbohydrase activities including inulinase and amylase were determined by the formation of clear zone around the colonies after soaking with Lugol on the TSA medium supplemented with 1% inulin powder and 1% starch. Similarly, the protease activities were determined by the formation of clear zone around the colonies on Skim Milk Agar Medium. Then, of the 11 isolates, only one known as UTMP 12 showed maximum enzyme activity. The isolate was then characterized based on morphology and biochemistry and found to be Bacillus subtilis strain JCM 1465 (accession number NR_113265, homolog 99.72%). Furthermore, this is the first study on carbohydrase and protease activities of Bacillus subtilis strain JCM 1465 and the result shows that the thermophilic bacteria are needed in the production of carbohydrase and protease.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Isolation and molecular identification of carbohydrase and protease producing Bacillus subtilis JCM 1465 isolated from Penen Hot Springs in North Sumatra, Indonesia

Fachrial¹,

Anggraini²,

Harmileni³

et al. 2019

Biodiversitas

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“…H7. These results are supported by the previous reports stated that the amylase production was increased when soluble starch was used as carbon sources [ 36 , 37 , 38 ].…”

Section: Discussionsupporting

confidence: 91%

Statistical Based Bioprocess Design for Improved Production of Amylase from Halophilic Bacillus sp. H7 Isolated from Marine Water

et al. 2021

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Amylase (EC 3.2.1.1) enzyme has gained tremendous demand in various industries, including wastewater treatment, bioremediation and nano-biotechnology. This compels the availability of enzyme in greater yields that can be achieved by employing potential amylase-producing cultures and statistical optimization. The use of Plackett–Burman design (PBD) that evaluates various medium components and having two-level factorial designs help to determine the factor and its level to increase the yield of product. In the present work, we are reporting the screening of amylase-producing marine bacterial strain identified as Bacillus sp. H7 by 16S rRNA. The use of two-stage statistical optimization, i.e., PBD and response surface methodology (RSM), using central composite design (CCD) further improved the production of amylase. A 1.31-fold increase in amylase production was evident using a 5.0 L laboratory-scale bioreactor. Statistical optimization gives the exact idea of variables that influence the production of enzymes, and hence, the statistical approach offers the best way to optimize the bioprocess. The high catalytic efficiency (kcat/Km) of amylase from Bacillus sp. H7 on soluble starch was estimated to be 13.73 mL/s/mg.

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“…Al-Johani ve ark. [18] optimuminkübasyon süresini 48 saat, optimum pH 8.5 ve sıcaklığı 45°C belirtmişlerdir. Asgher ve ark.…”

Section: Deterjan Etkisi 4 Sonuçlar Ve Tartışmaunclassified

Amylase Production of Bacillus subtilis Isolated from Soil by SmF Method

Ortakaya¹,

Fincan²

2019

BioDiCon

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In our study, the isolation, identification and optimization of Bacillus subtilis from soil samples were performed and the ability to produce α-amylase of industrial importance was investigated. Bacteria identified as B. subtilis by 16S rRNA analysis, morphological and biochemical tests. Maximum growth was determined at 32 hour, 37°C and pH 7.0. Enzyme optimization was performed after determination of bacterial amylase in starchy medium. Optimum conditions for amylase production were determined as 37°C, pH 7.0 and 48 h. When the effect of metals on enzyme activity was examined, it was determined that Cu2+ increased enzyme activity, Zn2+, Hg2+ and Fe2+ were partially inhibited the enzyme activity, and Ca2+ was showed close activity to the control. It was observed that carbon sources had inhibitory effect on bacterial growth and enzyme production.

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Optimization of Alkaline Α-Amylase Production by Thermophilic Bacillus Subtilis

Cited by 19 publications

References 19 publications

Isolation and molecular identification of carbohydrase and protease producing Bacillus subtilis JCM 1465 isolated from Penen Hot Springs in North Sumatra, Indonesia

Isolation and molecular identification of carbohydrase and protease producing Bacillus subtilis JCM 1465 isolated from Penen Hot Springs in North Sumatra, Indonesia

Statistical Based Bioprocess Design for Improved Production of Amylase from Halophilic Bacillus sp. H7 Isolated from Marine Water

Amylase Production of Bacillus subtilis Isolated from Soil by SmF Method

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