Optimising the dosage of ethyl methanesulphonate mutagenesis in selected wheat genotypes

OlaOlorun, Boluwatife M.; Shimelis, Hussein; Mathew, Isack; Laing, Mark

doi:10.1080/02571862.2019.1610808

Cited by 19 publications

(15 citation statements)

References 18 publications

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“…In the present study, the mean seedling emergence, survival rates and seedling height had significant reductions with increased EMS concentrations ( Table 2). This result corroborates the findings in rice [41], soybean [34] and wheat [42] which reported that seedling emergence, survival and growth were influenced by genetic variations and EMS concentrations.…”

Section: Effect Of Single Doses Of Emssupporting

confidence: 92%

The Effect of Single and Combined Use of Gamma Radiation and Ethylmethane Sulfonate on Early Growth Parameters in Sorghum

Wanga

Shimelis

Horn

et al. 2020

Plants

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Success in inducing genetic variation through mutagenic agents is dependent on the source and dose of application. The objective of this study was to determine the optimum doses of a single and combined use of gamma radiation and ethylmethane sulfonate (EMS) for effective mutation breeding in sorghum. The study involved two concurrent experiments as follows: in experiment I, the seeds of four sorghum genotypes (‘Parbhani Moti’, ‘Parbhani Shakti’, ‘ICSV 15013′, and ‘Macia’) were treated using gamma radiation (0, 300, 400, 500 and 600 Gy), EMS (0, 0.5 and 1.0%), and gamma radiation followed by EMS (0 and 300 Gy and 0.1% EMS; 400 Gy and 0.05% EMS). In experiment II, the seeds of two genotypes (‘Macia’ and ‘Red sorghum’) were treated with seven doses of gamma radiation only (0, 100, 200, 300, 400, 500 and 600 Gy). Overall, the combined applied doses of gamma radiation and EMS are not recommended due to poor seedling emergence and seedling survival rate below LD50. The best dosage of gamma radiation for genotypes Red sorghum, Parbhani Moti, Macia, ICSV 15013 and Parbhani Shakti ranged between 392 and 419 Gy, 311 and 354 Gy, 256 and 355 Gy, 273 and 304 Gy, and 266 and 297 Gy, respectively. The EMS optimum dosage ranges for genotypes Parbhani Shakti, ICSV 15013, Parbhani Moti and Macia were between 0.41% and 0.60%, 0.48% and 0.58%, 0.46% and 0.51%, and 0.36% and 0.45%, respectively. The above dose rates are useful to induce genetic variation in the tested sorghum genotypes for greater mutation events in sorghum breeding programs.

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Section: Effect Of Single Doses Of Emssupporting

confidence: 92%

The Effect of Single and Combined Use of Gamma Radiation and Ethylmethane Sulfonate on Early Growth Parameters in Sorghum

Wanga

Shimelis

Horn

et al. 2020

Plants

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“…Contrary to our results, seed germination was reduced to all doses of sodium azide in The results revealed that there is a linear association between increased doses of mutagens (SA and EMS) and reduced germination of seeds. This was also reported in black gram[20], wheat[21] and Okra[22]. The shoot length in two okra varieties was observed to exhibit marginal differences in mutagens treated and control.…”

supporting

confidence: 71%

Effect of chemical mutagens on growth of Okra (Abelmoschus esculentus L. Moench)

Nizamani

Rafiq

Kaleri

et al. 2020

PAB

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The purpose of the present study was to evaluate the effects of mutagens i.e. Ethyl methanesulfonate (EMS) and Sodium azide (SA) on the morphological growth of Okra. Seeds of two Okra varieties Subzpari and Pahuja were first pre-soaked in distilled water and then treated with 0.0 (Control), 0.1, 0.2 and 0.3% Sodium azide (SA) and Ethyl methanesulfonate (EMS) separately. The results showed that the germination rate was significantly decreased with increased lethality (%) and mutation frequency (%) in M1 of both varieties with increasing concentrations of EMS and SA against control. The results showed that different concentrations of EMS and SA affected morphological attributes of both okra varieties as the increase of mutagen concentration showed variable results in M1 generation compared with control, but M2 generation showed useful improvement in various traits. Such as the M2 generation of Subs pari from 0.1% SA treatment showed a decrease in flowering time while an increase in the number of fruits per plant, fruit size, seeds yield (g) per plant and 100 seeds weight.

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“…Induced mutation offers an opportunity to develop high‐performing and well‐adapted genotypes possessing useful agronomic traits such as seed size, plant height and seed yield as well as resistance/tolerance to abiotic and biotic stresses (Gwata, Shimelis, & Matova, 2016; Kumar, Katna, & Sharma, 2019). Different types of mutagens such as gamma rays and ethyl methane sulphonate (EMS) have been utilized to create genetic variation in cultivated crops (Hadebe, Modi, & Shimelis, 2017; Olaolorun, Shimelis, Mathew, & Laing, 2019). However, ethyl methane sulphonate is preferred as it creates large number of point mutations and its mutation frequency is independent of the genome size (Greene et al., 2003; Henikoff & Comai, 2003; McCallum, Comai, Greene, & Henikoff, 2000).…”

Section: Opportunities and Challenges For Development Of Tepary Bean mentioning

confidence: 99%

“…This shows that mutation breeding has a potential of creating sufficient genotypic and phenotypic variation for genetic improvement and selection of mutant lines for drought tolerance breeding. However, mutagenesis using EMS is reported to affect germination capacity (Olaolorun et al, 2019) and thus requires a dose formulation specific for each genotype (Gwata et al, 2016; Hadebe et al, 2017; Olaolorun et al, 2019).…”

Section: Opportunities and Challenges For Development Of Tepary Bean mentioning

confidence: 99%

Breeding tepary bean (Phaseolus acutifolius) for drought adaptation: A review

et al. 2020

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Tepary bean (Phaseolus acutifolius A. Gray) is a relatively higher drought‐tolerant crop than common bean (P. vulgaris), serving as genetic resource for food and genetic enhancement of related legumes. Tepary bean production is hampered by cultivation of low yielding and abiotic stress‐susceptible cultivars. Targeted selection of agronomic, physiological and biochemical traits that maximizes yield gains using Phaseolus gene pool is useful to develop stress‐tolerant and high‐performing genotypes. The objective of this review is to provide breeding progress made regarding tepary bean improvement for drought adaptation. Agronomic, physiological and biochemical traits utilized for selection of drought‐tolerant genotypes are highlighted. Genetic and genomic resources developed for tepary bean or closely related species such as common bean useful for genetic analysis and breeding are discussed. Opportunities and challenges to facilitate breeding of tepary bean genotypes with improved abiotic stress adaptation are highlighted. This will enable development of drought‐tolerant tepary bean genotypes targeting selection of agronomic, physiological and biochemical traits. Use of genetically related and complementary Phaseolus species and marker‐assisted selection method is key to developing drought‐tolerant genotypes.

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Optimising the dosage of ethyl methanesulphonate mutagenesis in selected wheat genotypes

Cited by 19 publications

References 18 publications

The Effect of Single and Combined Use of Gamma Radiation and Ethylmethane Sulfonate on Early Growth Parameters in Sorghum

The Effect of Single and Combined Use of Gamma Radiation and Ethylmethane Sulfonate on Early Growth Parameters in Sorghum

Effect of chemical mutagens on growth of Okra (Abelmoschus esculentus L. Moench)

Breeding tepary bean (Phaseolus acutifolius) for drought adaptation: A review

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