Optimisation of ultrasonic-assisted protein extraction from brewer's spent grain

Tang, De-Song; Tian, Ying-Juan; He, Yuanzhe; Lei, Lin; Hu, Songqing; Li, Bing

doi:10.17221/178/2009-cjfs

Cited by 54 publications

(30 citation statements)

References 29 publications

(26 reference statements)

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“…The concentration of soluble protein in UAE water extracts was 82 ± 1 mg protein/g BSG,dry . This value is of the same order as the one reported by Tang et al [23] in the study of UAE of protein fraction from BSG, with an optimum yield value of 104.2 mg protein/g BSG.…”

Section: Determination Of Extract Componentssupporting

confidence: 84%

Water Ultrasound-Assisted Extraction of Polyphenol Compounds from Brewer’s Spent Grain: Kinetic Study, Extract Characterization, and Concentration

et al. 2020

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Brewer’s spent grain (BSG) was chemically characterized obtaining 52.1% of carbohydrates, 17.8% protein, 5.9% lipids, 13.5% insoluble lignin and 24.3% of water-soluble extractives. This work has been focused on the study of polyphenol extraction of the extractive fraction by water ultrasound-assisted extraction. Selected extraction conditions were 47 °C and 21.7 mL water/gdry-BSG. The effect of solvent polarity on polyphenol extraction was studied by using ethanol aqueous mixtures, from 20% to 100% ethanol. The kinetics of polyphenol extraction have been fitted to the power law and the Weibull models yielding mean values of the root mean square deviation lower than 7.5%. Extracts have been characterized in terms of quantification of individual phenolic compounds by HPLC-DAD and protein and sugar soluble fractions (glucose, xylose, and arabinose). Polyphenol profile has been compared with other hydrolytic techniques, such as acid, basic and enzymatic hydrolysis, showing that ultrasound was not as effective as basic hydrolysis to release the phenolic acids esterified to the cell wall. A further centrifuge ultrafiltration concentration step was able to yield a retentate enriched in the protein fraction while individual phenolic compounds where mainly transferred to the permeate.

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Section: Determination Of Extract Componentssupporting

confidence: 84%

Water Ultrasound-Assisted Extraction of Polyphenol Compounds from Brewer’s Spent Grain: Kinetic Study, Extract Characterization, and Concentration

et al. 2020

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“…1b). In addition, Tang et al (2010) found that a higher yield of Brewer's spent grain protein was obtained from a lower solid-liquid ratio than in a higher ratio, which may be caused by the relatively higher protein content in the solution with a higher amount of the liquid than that in a higher ratio when the extraction time reached equilibrium. This phenomenon may be explained by the mass transfer principle and the distribution of ultrasonic energy in the solution, as well as by the lower ratio that has higher concentration gradient, which leads to a higher diffusion rate and extraction yield (Liu et al 2013).…”

Section: Model Fitting and Effects Of Independent Variable On Responsementioning

confidence: 98%

“…This phenomenon may be explained by the mass transfer principle and the distribution of ultrasonic energy in the solution, as well as by the lower ratio that has higher concentration gradient, which leads to a higher diffusion rate and extraction yield (Liu et al 2013). In addition, Tang et al (2010) found that a higher yield of Brewer's spent grain protein was obtained from a lower solid-liquid ratio than in a higher ratio, which may be caused by the relatively higher protein content in the solution with a higher amount of the liquid than that in a higher ratio when the extraction time reached equilibrium.…”

Section: Model Fitting and Effects Of Independent Variable On Responsementioning

confidence: 98%

Ultrasonic-Assisted Extraction of Rice Bran Protein Using Response Surface Methodology

Phongthai

Lim

Rawdkuen

2016

Journal of Food Biochemistry

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Rice bran was used for isolating protein through ultrasonic‐assisted extraction. The protein was converted into protein hydrolysates (PH) with three commercial enzymes. The optimal condition for rice bran protein production was 76% sonication amplitude, 18 min extraction and 0.99 g/10 mL solid–liquid ratio, which gave protein yield of 4.73 ± 0.03%. The degree of hydrolysis for the rice bran protein by Subtilisin A (SPH), Actinase E (APH) and Neutrase 0.8L (NPH) was 20.03 ± 0.24%, 13.84 ± 0.04% and 5.54 ± 0.07%, respectively. The molecular weight of the isolated proteins ranged between <11 to 75 kDa. The NPH exhibited greater scavenging activity on 1,1‐diphenyl‐2‐picryhydrazyl radical and ACE‐inhibitory activities. The SPH was efficient in reducing power and metal chelating activities. In addition, NHP displayed the highest positive effect for foaming activity (66.25%), then followed by APH (57.50%) and SPH (52.50%). The results suggest that the partial hydrolyzed rice bran protein is more suitable for application in food systems rather than the non‐hydrolyzed form. Practical Application Optimizing the condition for rice bran protein extraction is desirable and practical for related plant materials. Understanding the optimal conditions is important because it could enable an extraction method that could be used instead of conventional methods that take a long time for extraction. This study also suggests some enzymes for producing rice bran protein hydrolysates (PH) that have desired properties for specific usage such as for antioxidant activities and/or functional properties. The method for producing PH and controlling the degree of hydrolysis could be promptly applied.

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“…Sugars, for example are efficiently extracted from BSG at temperatures higher than 100°C, especially 120°C when using dilute acid [17] or between 150°C and 190°C when pure water is used as extraction solvent [30]. Proteins can be extracted from BSG by ultrasonic-assisted extraction [31] or ultrafiltratioin [32].…”

Section: Chemical Composition Of Bsg Extractsmentioning

confidence: 99%

Influence of extraction solvents on the recovery of antioxidant phenolic compounds from brewer’s spent grains

Meneses

Martins

Teixeira

et al. 2013

Separation and Purification Technology

325

224

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This study evaluated the efficacy of different solvents (methanol, ethanol, acetone, hexane, ethyl acetate, water, methanol:water mixtures, ethanol:water mixtures, and acetone:water mixtures) for extracting antioxidant phenolic compounds from brewer's spent grains (BSGs). The extracts were characterized regarding the contents of total phenols, flavonoids, proteins and reducing sugars. Antioxidant activity was determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical, and the ferric reducing antioxidant power (FRAP) assay. The solvents had different efficiencies for extraction of antioxidant phenolic compounds. All the produced extracts showed antioxidant activity, but the extract produced with 60% v/v acetone had the most elevated content of total phenols and antioxidant potential by the two methods. BSG was demonstrated to be a valuable source of antioxidant phenolic compounds, and solid-to-liquid extraction using 60% v/v acetone was a low cost and quite efficient method to recover these value-added compounds.

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Optimisation of ultrasonic-assisted protein extraction from brewer's spent grain

Cited by 54 publications

References 29 publications

Water Ultrasound-Assisted Extraction of Polyphenol Compounds from Brewer’s Spent Grain: Kinetic Study, Extract Characterization, and Concentration

Water Ultrasound-Assisted Extraction of Polyphenol Compounds from Brewer’s Spent Grain: Kinetic Study, Extract Characterization, and Concentration

Ultrasonic-Assisted Extraction of Rice Bran Protein Using Response Surface Methodology

Influence of extraction solvents on the recovery of antioxidant phenolic compounds from brewer’s spent grains

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