Optimal incubating time of in vitro bromodeoxyuridine labeling of human umbilical cord blood- mononuclear cells and their functional assessment in ICH rats

Seghatoleslam, Masoumeh; Jalali, Mehdi; Alamdari, Daryoush Hamidi; Nikravesh, Mohammad Reza; Hoseini, Mahmoud; Fazel, Alireza; Koliakos, George

doi:10.5897/jcab12.009

Cited by 3 publications

(2 citation statements)

References 47 publications

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“…MSCs were marked by incorporation of 5-bromo-2 deoxyuridine (BrdU; Sigma-Aldrich, Taufkirchen, Germany). MSCs were incubated with BrdU (10 μ M/L) for 48 h at 37°C to label more than 90% of the incubated cells ( 18 ).…”

Section: Methodsmentioning

confidence: 99%

Study of the Effect of Route of Administration of Mesenchymal Stem Cells on Cisplatin-Induced Acute Kidney Injury in Sprague Dawley Rats

Moustafa

Sobh

Abouelkheir

et al. 2016

IJSC

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Background and ObjectivesMesenchymal stem cells (MSCs) have been shown to ameliorate cisplatin-induced acute kidney injury (AKI). The present study compares the efficacy of different routes of MSCs administration on kidney damage and regeneration after cisplatin-induced AKI.MethodsA single intraperitoneal injection of cisplatin (5 mg/kg) was used to induce AKI in 160 rats. MSCs (5×106) were given by either intravenous, intra-arterial or kidney sub capsular injection one day after cisplatin injection. Suitable control groups were included. Rats were sacrificed at 4, 7, 11 and 30 days after cisplatin injection. Kidney function parameters, kidney tissue oxidative stress markers, and scoring for renal tissue injury, regeneration and chronicity were all determined.ResultsMSCs by any routes were able to ameliorate kidney function deterioration and renal tissue damage induced by cisplatin. The overall results of the three routes were equal. Differences between the different routes in one parameter were transient and inconsistent with other parameters.ConclusionsChanging the route of MSCs injection does not have a major influence on the outcome. Future evaluation should focus on differences between the routes of administration considering the long term safety.

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Section: Methodsmentioning

confidence: 99%

Study of the Effect of Route of Administration of Mesenchymal Stem Cells on Cisplatin-Induced Acute Kidney Injury in Sprague Dawley Rats

Moustafa

Sobh

Abouelkheir

et al. 2016

IJSC

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“…Before transplantation of ADMSCs, and when the cells reached 80-90% confluence in the 3 rd passage, cells were incubated for 2 days at 37˚C and 5% CO2 in DMEM supplemented with 10 μm/l BrdU (5-bromo-2 deoxyuridine) (Thermo scientific, USA), and then the cells were collected within 30 min and were suspended in normal saline before transplanting into the rats [11].…”

Section: Identification and Homing Of Admscsmentioning

confidence: 99%

Administration of Adipose derived mesenchymal stem cells promotes amelioration of renal function in cisplatin-induced acute kidney injury in rats.

El‐Deen

Zahran

Barakat

et al. 2020

Alfarama Journal of Basic & Applied Sciences

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Cisplatin is a chemotherapeutic agent, causes nephrotoxicity when it was administered for long periods. Our study conducted an investigation into the effect of ADMSCs in attenuation of cisplatin induced AKI. A total of 90 Sprague-Dawley rats (180-220 g) were apportioned to three groups: control group, cisplatin group, and ADMSCs + CP group. Ten rats were sacrificed on the days 3, 7 and 11, the kidney tissues and blood samples were obtained. Renal functions, oxidative stress parameters, molecular studies and histopathological studies were analyzed, as well as ADMSCs isolation and characterization was done.Cisplatin injection caused disturbance in kidney function through increasing serum creatinine, blood urea nitrogen and MDA, and decreasing GSH activity. The amelioration in renal function appeared at day 3 with the use of ADMSCs. Injection of cisplatin induced tubular apoptosis and inflammation by increasing Caspase-3 and NF KB , in addition to tubular degenerations evaluated by pathological score, in contrast, ADMSCs group showed a significantly lowered inflammation and apoptosis at days 3, 7, 11. This study concluded that ADMSCs have reno-protective effects that can be explained by three possible mechanisms of action, targeting oxidative stress, targeting apoptosis and reducing inflammation through which it could improve cisplatin toxicity.

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The role of bone marrow derived mesenchymal stem cells in induced stroke

Waheed¹,

Karim²,

Gali³

2014

Afr. J. Biotechnol.

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The ro Int 3 D Stroke is th Recovery a mesenchym was directe as to study for Middle possess th positive res surface ma intravenous MCAO in m in the cereb tissues. In may provid Key word bromodeoxy TRODUCTION ne marrow d SCs), like ot imited self-re ls from variou Corresponding a uthor(s) agree t ternational Lice bbreviations:. 4399-4409, 19 AJB2014.13751 r: 0504FEE4862 5 014 n the copyrigh cademicjourn Research ole of b tissar Num 1 Depa 2 D Department of he third mos after a maj mal stem cell ed to isolatio y the role of Cerebral Ar he ability to sponse for arker (CD34¯) s administra mature rats, d bral cortex a conclusion, e a cell sour ds: Middle yyuridin. N derived mes ther stem ce newal and th us cell lineage author.

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Optimal incubating time of in vitro bromodeoxyuridine labeling of human umbilical cord blood- mononuclear cells and their functional assessment in ICH rats

Cited by 3 publications

References 47 publications

Study of the Effect of Route of Administration of Mesenchymal Stem Cells on Cisplatin-Induced Acute Kidney Injury in Sprague Dawley Rats

Study of the Effect of Route of Administration of Mesenchymal Stem Cells on Cisplatin-Induced Acute Kidney Injury in Sprague Dawley Rats

Administration of Adipose derived mesenchymal stem cells promotes amelioration of renal function in cisplatin-induced acute kidney injury in rats.

The role of bone marrow derived mesenchymal stem cells in induced stroke

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