Optical validation of<i>in vitro</i>extra-cellular neuronal recordings

Herzog, Nicole; Shein-Idelson, Mark; Hanein, Yael

doi:10.1088/1741-2560/8/5/056008

Cited by 25 publications

(22 citation statements)

References 33 publications

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“…Imaging and electrophysiological studies have shown that (because of low impedance of the medium surrounding the neurons) the electrodes of the MEA can only capture activity of neurons that lie in very close vicinity to the electrodes (e.g., Herzog et al 2011). We have observed the same phenomena by patch-clamping single cells at Conditions 1-8 denote the drugs that were applied and (consequently) the receptors that remained functional.…”

Section: Cross-correlationmentioning

confidence: 53%

Network burst activity in hippocampal neuronal cultures: the role of synaptic and intrinsic currents

Suresh

Radojicic

Pesce

et al. 2016

Journal of Neurophysiology

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AK, Marks JD, van Drongelen W. Network burst activity in hippocampal neuronal cultures: the role of synaptic and intrinsic currents. J Neurophysiol 115: 3073-3089, 2016. First published March 16, 2016 doi:10.1152/jn.00995.2015.-The goal of this work was to define the contributions of intrinsic and synaptic mechanisms toward spontaneous network-wide bursting activity, observed in dissociated rat hippocampal cell cultures. This network behavior is typically characterized by short-duration bursts, separated by order of magnitude longer interburst intervals. We hypothesize that while shorttimescale synaptic processes modulate spectro-temporal intraburst properties and network-wide burst propagation, much longer timescales of intrinsic membrane properties such as persistent sodium (Na p ) currents govern burst onset during interburst intervals. To test this, we used synaptic receptor antagonists picrotoxin, 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), and 3-(2-carboxypiperazine-4-yl)propyl-1-phosphonate (CPP) to selectively block GABA A , AMPA, and NMDA receptors and riluzole to selectively block Na p channels. We systematically compared intracellular activity (recorded with patch clamp) and network activity (recorded with multielectrode arrays) in eight different synaptic connectivity conditions: GABA A ϩ NMDA ϩ AMPA, NMDA ϩ AMPA, GABA A ϩ AMPA, GABA A ϩ NMDA, AMPA, NMDA, GABA A , and all receptors blocked. Furthermore, we used mixed-effects modeling to quantify the aforementioned independent and interactive synaptic receptor contributions toward spectro-temporal burst properties including intraburst spike rate, burst activity index, burst duration, power in the local field potential, network connectivity, and transmission delays. We found that blocking intrinsic Na p currents completely abolished bursting activity, demonstrating their critical role in burst onset within the network. On the other hand, blocking different combinations of synaptic receptors revealed that spectro-temporal burst properties are uniquely associated with synaptic functionality and that excitatory connectivity is necessary for the presence of network-wide bursting. In addition to confirming the critical contribution of direct excitatory effects, mixed-effects modeling also revealed distinct combined (nonlinear) contributions of excitatory and inhibitory synaptic activity to network bursting properties. epilepsy; multielectrode arrays; network bursting; pharmacology; synaptic mechanisms SPONTANEOUS NETWORK-WIDE SYNCHRONIZED bursting activity appears to play an important role in several aspects of the nervous system including development (Gu and Spitzer 1995;Meister et al. 1991;Shatz 1990;Yuste et al. 1992) and integration in the sensory system (Engel et al. 1992) but also in the initiation of pathological activity such as epileptic seizures (Gutnick et al. 1982;Miles and Wong 1983). Therefore, clarifying how the underlying synaptic and intrinsic neuronal properties modulate and cause this network behavior is likely to provide valuable under...

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Section: Cross-correlationmentioning

confidence: 53%

Network burst activity in hippocampal neuronal cultures: the role of synaptic and intrinsic currents

Suresh

Radojicic

Pesce

et al. 2016

Journal of Neurophysiology

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“…5(d), the suppression level increased monotonically as the pattern size increased. Considering that an electrode captures neural activity from cells within close distance (60 μm) [27], adjusting the diameter of circular illumination pattern might be controlling the number of neurons that influence the recording neuron. The inhibition was also dependent upon the distance from the illumination area, which made it possible to obtain area-specific neural inhibition.…”

Section: Discussionmentioning

confidence: 99%

Digital micromirror based near-infrared illumination system for plasmonic photothermal neuromodulation

Jung

Kang

Nam

2017

Biomed. Opt. Express

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Light-mediated neuromodulation techniques provide great advantages to investigate neuroscience due to its high spatial and temporal resolution. To generate a spatial pattern of neural activity, it is necessary to develop a system for patterned-light illumination to a specific area. Digital micromirror device (DMD) based patterned illumination system have been used for neuromodulation due to its simple configuration and design flexibility. In this paper, we developed a patterned near-infrared (NIR) illumination system for region specific photothermal manipulation of neural activity using NIR-sensitive plasmonic gold nanorods (GNRs). The proposed system had high power transmission efficiency for delivering power density up to 19 W/mm 2 . We used a GNR-coated microelectrode array (MEA) to perform biological experiments using E18 rat hippocampal neurons and showed that it was possible to inhibit neural spiking activity of specific area in neural circuits with the patterned NIR illumination. This patterned NIR illumination system can serve as a promising neuromodulation tool to investigate neuroscience in a wide range of physiological and clinical applications. 16 (7), 805-815 (2013). 18. Y. M. Tamar Arens-Arad, N. Faraha, S. Ben-Yaishc, and A. Zlotnikc, "Head mounted DMD based projection system for natural and prosthetic visual stimulation in freely moving rats," Sci.

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“…Cultures were prepared as described in Herzog et al (2011). After the fourth day in vitro , the growth medium was enriched with 0.5% Pen-Strep (Biological Industries Beit Haemek), 2% B-27 (Gibco), and 0.75% glutamax (Biological Industries Beit Haemek).…”

Section: Methodsmentioning

confidence: 99%

In vitro large-scale experimental and theoretical studies for the realization of bi-directional brain-prostheses

Bonifazi¹,

Difato²,

Massobrio³

et al. 2013

Front. Neural Circuits

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Brain-machine interfaces (BMI) were born to control “actions from thoughts” in order to recover motor capability of patients with impaired functional connectivity between the central and peripheral nervous system. The final goal of our studies is the development of a new proof-of-concept BMI—a neuromorphic chip for brain repair—to reproduce the functional organization of a damaged part of the central nervous system. To reach this ambitious goal, we implemented a multidisciplinary “bottom-up” approach in which in vitro networks are the paradigm for the development of an in silico model to be incorporated into a neuromorphic device. In this paper we present the overall strategy and focus on the different building blocks of our studies: (i) the experimental characterization and modeling of “finite size networks” which represent the smallest and most general self-organized circuits capable of generating spontaneous collective dynamics; (ii) the induction of lesions in neuronal networks and the whole brain preparation with special attention on the impact on the functional organization of the circuits; (iii) the first production of a neuromorphic chip able to implement a real-time model of neuronal networks. A dynamical characterization of the finite size circuits with single cell resolution is provided. A neural network model based on Izhikevich neurons was able to replicate the experimental observations. Changes in the dynamics of the neuronal circuits induced by optical and ischemic lesions are presented respectively for in vitro neuronal networks and for a whole brain preparation. Finally the implementation of a neuromorphic chip reproducing the network dynamics in quasi-real time (10 ns precision) is presented.

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Optical validation ofin vitroextra-cellular neuronal recordings

Cited by 25 publications

References 33 publications

Network burst activity in hippocampal neuronal cultures: the role of synaptic and intrinsic currents

Network burst activity in hippocampal neuronal cultures: the role of synaptic and intrinsic currents

Digital micromirror based near-infrared illumination system for plasmonic photothermal neuromodulation

In vitro large-scale experimental and theoretical studies for the realization of bi-directional brain-prostheses

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