2015
DOI: 10.1017/cbo9781107279711
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Optical Tweezers

Abstract: Combining state-of-the-art research with a strong pedagogic approach, this text provides a detailed and complete guide to the theory, practice and applications of optical tweezers. In-depth derivation of the theory of optical trapping and numerical modelling of optical forces are supported by a complete step-by-step design and construction guide for building optical tweezers, with detailed tutorials on collecting and analysing data. Also included are comprehensive reviews of optical tweezers research in fields… Show more

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Cited by 336 publications
(224 citation statements)
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“…The laser power at each trapping site is kept low enough (≃2 mW) to avoid significant heating ( (ref. 40 )). The DVM uses a monochromatic camera acquiring videos at 200 fps; these frames are analysed using standard DVM algorithms to determine the projected positions of the particles with nanometric accuracy 35 36 (see Methods for details).…”
Section: Resultsmentioning
confidence: 99%
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“…The laser power at each trapping site is kept low enough (≃2 mW) to avoid significant heating ( (ref. 40 )). The DVM uses a monochromatic camera acquiring videos at 200 fps; these frames are analysed using standard DVM algorithms to determine the projected positions of the particles with nanometric accuracy 35 36 (see Methods for details).…”
Section: Resultsmentioning
confidence: 99%
“…where r i is the position of the centre of particle i , Δ r i = r i − R i , and R i is the position of the centre of the i th trap, while d ij =| r i − r j |−2 R is the distance between the surfaces of particles i and j . The optical traps are harmonic 40 , that is,…”
Section: Resultsmentioning
confidence: 99%
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“…The image displayed on the screen corresponds to the amplitude or phase pattern displayed on the device. There are numerous guides for setting up one of these devices in an optical tweezers system [13,14], and many of these guides describe generating SLM patterns for beam shaping but they do not provide easy to use codes.…”
Section: Device Overview and Operationmentioning
confidence: 99%