Optical Fiber-Mediated Immunosensor with a Tunable Detection Range for Multiplexed Analysis of Veterinary Drug Residues

Nie, Rongbin; Xu, Xuexue; Chen, Yiping; Yang, Li

doi:10.1021/acssensors.9b00653

Cited by 30 publications

(10 citation statements)

References 26 publications

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“…The optical fiber sensor was constructed according to the method described in our previous study . The detailed procedure is presented in the Supporting Information.…”

Section: Methodsmentioning

confidence: 99%

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Immunoassays Using Optical-Fiber Sensor with All-Directional Chemiluminescent Collection

Nie

Huang

et al. 2020

Anal. Chem.

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While chemiluminescent optical-fiber sensors present essential advantages for immunoassays of biomarkers, an inherent limitation hampering their detection sensitivity is the low collection efficiency of chemiluminescent emission induced by samples, owing to the microspatial scale of an optical fiber for transmission of light via total internal reflection. Here we present a robust approach to overcome this limitation, based on a uniquely designed all-optical chemiluminescent collection vial (CC vial) by using a concave mirror and a coaxial tubular mirror as its bottom and wall, respectively. Using cardiac troponin I (cTnI), a highly specific but low abundance cardiac biomarker, as the test sample, we show that accurate assays can be achieved in a wide linear range of 1–80000 pg/mL. The limit of detection is as low as 0.31 pg/mL, which is about 2 orders of magnitude lower than that obtained by a normal chemiluminescent optical fiber sensor. The method is successfully applied for determination of cTnI in real human serum samples with good accuracy and repeatability. Our study shows that the method can perform an all-directional collection of the chemiluminescent emission, thus, greatly enhancing the collection efficiency and improving the sensitivity of the immunoassays. It is also worth mentioning that the proposed strategy requires neither complex equipment nor additional chemicals, making it a cost-effective and universal approach for ultrasensitive detection of trace biomarkers.

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“…The optical fiber sensor was constructed according to the method described in our previous study . The detailed procedure is presented in the Supporting Information.…”

Section: Methodsmentioning

confidence: 99%

“…The optical fiber sensor was constructed according to the method described in our previous study. 21 The detailed procedure is presented in the Supporting Information. Immunoassay using the sensor was based on a sandwich approach with chemiluminescent detection.…”

mentioning

confidence: 99%

Immunoassays Using Optical-Fiber Sensor with All-Directional Chemiluminescent Collection

Nie

Huang

et al. 2020

Anal. Chem.

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Section: Colorimetric Indicatorsmentioning

confidence: 99%

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confidence: 99%

Smart Materials Enabled with Artificial Intelligence for Healthcare Wearables

Zheng

Tang

Omar

et al. 2021

Adv Funct Materials

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Contemporary medicine suffers from many shortcomings in terms of successful disease diagnosis and treatment, both of which rely on detection capacity and timing. The lack of effective, reliable, and affordable detection and real-time monitoring limits the affordability of timely diagnosis and treatment. A new frontier that overcomes these challenges relies on smart health monitoring systems that combine wearable sensors and an analytical modulus. This review presents the latest advances in smart materials for the development of multifunctional wearable sensors while providing a bird's eye-view of their characteristics, functions, and applications. The review also presents the state-of-the-art on wearables fitted with artificial intelligence (AI) and support systems for clinical decision in early detection and accurate diagnosis of disorders. The ongoing challenges and future prospects for providing personal healthcare with AI-assisted support systems relating to clinical decisions are presented and discussed.

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“…The preparation of biotinylated-Ab 2 conjugates was based on our previous work . First, both sulfo-NHS-LC-biotin and Ab 2 were diluted to 1 mg/mL using DMF and PBS (pH = 7.4, 10 mM), respectively.…”

Section: Experimental Sectionmentioning

confidence: 99%

Direct Transverse Relaxation Time Biosensing Strategy for Detecting Foodborne Pathogens through Enzyme-Mediated Sol–Gel Transition of Hydrogels

et al. 2021

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In this work, we develop a direct transverse relaxation time (T 2) biosensing strategy and employ it for assaying foodborne pathogens relying on the alkaline phosphatase (ALP)-mediated sol–gel transition of hydrogels. ALP can catalyze the reaction to generate an acidic environment to transform the sol-state alginate solution to hydrogel, and this hydrogelation process can directly regulate the diffusion rate of water protons that results in a T 2 change of water molecules. By means of enzyme-modulated sol–gel transition and antigen–antibody interactions, this T 2 biosensor displays high sensitivity for detecting 50 CFU/mL S. enteritidis within 2 h. This biosensing strategy directly modulates the water molecules rather than magnetic probes in traditional methods, offering a straightforward, novel, and sensitive platform for pathogen detection.

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Optical Fiber-Mediated Immunosensor with a Tunable Detection Range for Multiplexed Analysis of Veterinary Drug Residues

Cited by 30 publications

References 26 publications

Immunoassays Using Optical-Fiber Sensor with All-Directional Chemiluminescent Collection

Immunoassays Using Optical-Fiber Sensor with All-Directional Chemiluminescent Collection

Smart Materials Enabled with Artificial Intelligence for Healthcare Wearables

Direct Transverse Relaxation Time Biosensing Strategy for Detecting Foodborne Pathogens through Enzyme-Mediated Sol–Gel Transition of Hydrogels

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